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QuantiFluor® ssDNA System

Sensitive Quantitation of Single-Stranded DNA

  • More sensitive than NanoDrop for low-concentration samples
  • Requires less DNA than spectrophotometry

Size

Catalog number selected: E3190

$ 301.00
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QuantiFluor® ssDNA System
1ml
$ 301.00
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Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.

The QuantiFluor® ssDNA System contains a fluorescent dye that enables sensitive quantitation of small amounts of single-stranded (ssDNA) in solution. Detecting and quantitating ssDNA is useful many molecular biology research applications, including studying ssDNA viruses, quantitating short synthetic ssDNA probes for site-directed mutagenesis, analysis of first-strand cDNAs, and quantitating bisulfate-treated DNA to study DNA methylation.

The QuantiFluor® ssDNA System requires less DNA (200pg) than other methods (~1 µg), so you save more of your sample for downstream assays.

QuantiFluor ssDNA Kit

Improved Sensitivity vs. Absorbance and Other Fluorescent ssDNA Quantitation Systems

For low-concentration samples, sensitivity of the QuantiFluor® ssDNA System is significantly increased compared to absorbance at 260nm (NanoDrop® method) and Qubit® ssDNA.

The dynamic range of the assay is wider than that of Qubit® ssDNA and equivalent to OliGreen® reagent.

12116MB-W
Sample*Assay
QuantiFluor® ssDNA System
0.2–400ng/µl
0.2–400ng
Quant-iT™ OliGreen
0.2–400ng/µl
0.2–400ng
Qubit® ssDNA
1–200ng/µl
1–200ng
NanoDrop® 2000 Spectrophotometer
2–12,000ng/µl
2–12,000ng

*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.


ssDNA Quantitation Sensitivity and Assay Linearity

The QuantiFluor® ssDNA System detects as little as 1ng/ml ssDNA in a 96-well microplate assay (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU. Sensitivity is equivalent to Quanti-IT™ OliGreen® reagent.

10995MC-W

Easy Setup on Microplate or Single-Tube Fluorometers

The QuantiFluor® ssDNA System includes all the necessary reagents to quickly set up and quantitate single-stranded DNA, and is easy to set up on microplate or single-tube fluorometers. The System is compatible with any fluorometer capable of measuring the appropriate fluorescence excitation and emission spectra. Compatible Promega fluorometers include:

Quantus Nucleic Acid Quantification Fluorometer

(Use Blue Channel Ex 460nm, Em 515–575nm)

GloMax-Multi-Jr-332x302

GloMax®-Multi Jr

(With Cat.# E6071 Fluorescence Optical Kit – BLUE; Ex 460nm, Em 515–570nm)


How Do Fluorescent Dyes Compare to Other Quantitation Methods?

In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

UV-Absorbance vs Fluorescence

DNA Extraction UV Absorbance vs Fluorescent Dyes

Fluorescence vs qPCR

DNA Purification Fluorescent Dyes vs qPCR

Specifications

What's in the box?

Item Part # SizeAvailable Separately

20X TE Buffer (pH 7.5)

E260A 1 × 25ml View Product

QuantiFluor® ssDNA Dye, 200X

E284A 1 × 1ml

ssDNA Standard

E288A 1 × 100μg

SDS

Choose language:

Download SDSPDF (96 KB) – English (United States)

Certificate of Analysis

See all certificates for E3190

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

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