QuantiFluor® Dx dsDNA System

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IVD for Quantitation of Double-Stranded DNA in Solution

  • Sensitive and specific quantitation of dsDNA
  • Suitable for use with in vitro diagnostic assays, including the OncoMate® MSI Dx Analysis System
  • IVD Medical Device


Catalog number selected: E5900

$ 422.00
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QuantiFluor® Dx dsDNA System
2,000 assays
$ 422.00
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Simple, Accurate DNA Quantitation for Diagnostic Assays

The QuantiFluor® Dx dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantification of small amounts of double-stranded DNA (dsDNA) in a purified sample. The system can be used with both single-tube and plate fluorometers that are capable of excitation in the range of 475–505nm and measuring emission in the range of 520–550nm.

Diagram showing how the quantifluor DX DNA binding dye is used to quantify DNA in diagnostic assays.

Reliable Quantitation for the OncoMate® MSI Dx Analysis System Workflow

The QuantiFluor® Dx dsDNA System is an ideal option for the OncoMate® MSI Dx Analysis System, a fluorescent, multiplex PCR-based test to detect microsatellite instability (MSI) in colorectal cancer tumor samples.

Illustration of a Maxwell CSC Instrument for DNA Isolation
Isolate DNA

from FFPE samples using the Maxwell® CSC DNA FFPE Kit and the Maxwell® CSC Instrument

Illustration of a Quantus Fluorometer
Quantitate DNA

using fluorescent DNA quantitation reagents (e.g.,  QuantiFluor® Dx dsDNA System) and instruments

Illustration of a PCR amplification tube and pipette tip
Amplify DNA

using the OncoMate® MSI Dx Analysis System

Illustration of an electropharogram for CE calibration
Calibrate the Dye Spectrum

using the OncoMate® 5C Matrix Standards

Illustration of a capillary electrophoresis instrument
Separate and Detect Fragments

using the Applied Biosystems® 3500 Dx Genetic Analyzer

Illustration of an electropherogram the OncoMate MSI Dx Analysis System
Data Analysis and Reporting

using the OncoMate® MSI Dx Interpretive Software

Robust Performance to Support Your Diagnostic Workflows

The QuantiFluor® Dx dsDNA System demonstrated robust performance in a clinical setting, generating DNA quantification values to enable valid results with an amplification-based test, the OncoMate® MSI Dx Analysis System, and further comparison to an orthogonal method. Suitable DNA concentration values for 308 FFPE tumor and normal tissue samples from various sources and exceptional storage duration (>10 years) were generated and contributed to the low sample reprocessing observed for the OncoMate® MSI Dx Analysis System method comparison clinical study.


The QuantiFluor® Dx dsDNA System provided predictive quantification for 308 DNA eluates from formalin-fixed, paraffin-embedded (FFPE) tumor and normal tissue samples.

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Medical Information

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Intended Use Statement

The QuantiFluor® Dx dsDNA System is a fluorescent dye-based test intended to quantify double-stranded DNA (dsDNA) in a sample containing purified nucleic acid. Fluorescent output is compared to results from a standard curve to calculate a dsDNA concentration. The dsDNA concentration results from the QuantiFluor® Dx dsDNA System are suitable for use in in vitro diagnostic assays.

The QuantiFluor® Dx dsDNA System is intended for use, in combination with a suitable fluorometer, to perform double-stranded DNA (dsDNA) quantification. The quantification data obtained using the QuantiFluor® Dx dsDNA System is suitable for use in preparation of dsDNA samples intended to be tested for in vitro diagnostic purposes.

The QuantiFluor® Dx dsDNA System is not intended for use as part of a specific in vitro diagnostic test. The QuantiFluor® Dx dsDNA System is intended for professional use only. Diagnostic results obtained using the quantification data identified with this system must be interpreted in conjunction with other clinical or laboratory data.


What's in the box?

Item Part # Size Available Separately

QuantiFluor® dsDNA Dye

E258A 1 × 1ml

Lambda DNA Standard

E259A 1 × 100μg

20X TE Buffer (pH 7.5)

E260A 1 × 25ml View Product

Certificate of Analysis

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Use Restrictions

For In Vitro Diagnostic Use. This product is only available in certain countries.

Storage Conditions


Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.


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