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T Cell Activation Bioassay (TCRαβ-KO)

Measure Potency of Transgenic T Cell Receptors

  • Amenable to high-throughput formats
  • Uses Bio-Glo-NL™ Luciferase Assay System

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T Cell Activation Bioassay (TCRαβ-KO)
TCRαβ-KO (CD4+)/CPM
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TCRαβ-KO: T Cell Activation Bioassay for Transgenic TCRs

Adoptive T cell immunotherapy is an important cancer treatment strategy in which genetically modified T cells are made tumor-specific and activated via introduction of a transgenic antigen receptor, which improves their ability to recognize and destroy tumors. T cell receptor-engineered T cell (TCR-T) and chimeric antigen receptor T cell (CAR-T) therapy are two types of adoptive T cell immunotherapy.

Measurement of TCR activation using traditional methods relies on laborious protocols and highly variable end points. The TCRαβ-KO Bioassay overcomes the limitations of these existing methods and enables functional testing of transgenic TCRs for antigen ranking, specificity and safety testing for applications such as quality control, TCR-T and CAR-T therapies.

The T Cell Activation Bioassay (TCRαβ-KO) is a bioluminescent cell-based assay for measuring the potency of transgenic TCR constructs to activate T cells without the constraints of endogenous TCR expression. The assay consists of a genetically engineered Jurkat T cell line with endogenous TCR α and β chains knocked out using CRISPR/Cas9. These cells express a luciferase reporter driven by a TCR pathway-dependent promoter.

Advantages

  • Prequalified according to ICH guidelines: The bioassays demonstrate the precision, accuracy and linearity required for routine use in potency and stability studies.
  • Simple and robust workflow: Easy to implement with no specialized skills or training required.
  • Amenable to antibody screening and drug discovery: Run the bioassay in 96-well and 384-well plate formats.

Applications

  • Determine the activity of CAR-T cell receptors.
  • Detect the activity and specificity of antigens.
  • Measure bispecific antibody potency and stability.

Assay Components

The CPM format of TCRαβ-KO CD8+ (Cat.# GA1162), TCRαβ-KO CD4+ (Cat.# GA1172) and TCRαβ-KO CD4+/CD8+ (Cat.# GA1182) contains cryopreserved cells that can be thawed, propagated and banked for long-term use. The bioluminescent signal is detected and quantified using the Bio-Glo-NL™ Luciferase Assay System (Cat.# J3081, J3082, J3083) and a standard luminometer such as the GloMax® Discover System (Cat.# GM3000). The propagation model is intended to be used with user-provided TCR constructs.

The assay is also available in a Cell Bank format (Cat.# GA1220, GA1210, GA1230).

Assay Principle

The T Cell Activation Bioassay (TCRαβ-KO), Propagation Model, is a bioluminescent reporter cell-based assay that overcomes the limitations of existing assays. Introduction of TCR α and β chains into the TCRαβ-KO Cells results in transgenic TCR expression on the cell surface. Activation of transgenic TCR-expressing TCRαβ-KO Cells by cognate peptide and major histocompatibility complex (MHC)-expressing antigen-presenting cells (APCs) results in potent TCR activation and promoter-mediated luminescence.

T cell activation assay principle

Representation of the T Cell Activation Bioassay (TCRαβ-KO). Without expressing a transgenic TCR, the TCRαβ-KO Cells are not activated by peptide or MHC, resulting in low light output. TCRαβ-KO Cells transfected or transduced to express a TCR are activated by APCs and cognate antigen, inducing TCR pathway-activated luminescence.

Assay Performance Data

T cell activation assay data (TCRαβ-KO, CD4+)
T cell activation assay data (TCRαβ-KO, CD4+)

Representative data for the T Cell Activation Bioassay (TCRαβ-KO, CD4+). Panel A. TCR-transfected parental Jurkat cells (endogenous CD4+) and TCRαβ-KO (CD4+) cells were cocultured with HLA-DR-positive cells and a titration of cognate peptide. After incubation, Bio-Glo-NL™ Reagent was added and luminescence quantified using the GloMax® Discover System. Panel B. TCRαβ-KO (CD4+) Cells were transfected with the Positive Control TCR Plasmid (or mock transfected) using the Lonza Nucleofector™ System. Forty-eight hours after transfection, the cells were cocultured with MHCII APC Cells and a titration of the Positive Control Peptide. After a 6-hour induction at 37°C, Bio-Glo-NL™ Reagent was added and luminescence measured using the GloMax® Discover System.

TCRαβ-KO Bioassay is available in CPM and Cell Bank formats:

  • TCRαβ-KO Cell Line (CD4+), Propagation Model (Cat.# GA1172)
  • TCRαβ-KO Cell Line (CD8+), Propagation Model (Cat.# GA1162)
  • TCRαβ-KO Cell Line (CD4+/CD8+), Propagation Model (Cat.# GA1182)
  • TCRαβ-KO Cells (CD4+), Cell Bank (Cat.# GA1210)
  • TCRαβ-KO Cells (CD8+), Cell Bank (Cat.# GA1220)
  • TCRαβ-KO Cells (CD4+/CD8+), Cell Bank (Cat.# GA1230)

TCR Transfection Positive Control Kit is available separately (e-mail EliteAccess@promega.com)

Specifications

You are viewing: GA1172 Change Configuration

What's in the box?

Item Part # Size

TCRαβ-KO (CD4+) Cells (CPM)

GA117A 2 × 1ml

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

By purchase of this material, Promega Corporation (“Promega”) conveys to purchaser (the “Limited Licensee”) the non-transferable right to use the material purchased from Promega and derivatives made by Limited Licensee solely for research conducted by Limited Licensee in accordance with the following requirements: (i) Limited Licensee shall not sell or otherwise transfer material or derivatives to any other person or entity, or use material or derivatives to perform services for the benefit of any other person or entity, except to transfer the material or derivatives to a Limited Licensee affiliate provided such affiliate agrees to be bound by the terms of this Limited License; (ii) Limited Licensee shall use material and derivatives only for its internal research within the Customer Field, which may include internal research within the Customer Field in connection with product research, and not for any Commercial Purposes; (iii) Limited Licensee shall use material and derivatives in compliance with all applicable laws and regulations, including without limitation applicable human health and animal welfare laws and regulations; (iv) Institutions shall provide no warranties of any kind to Limited Licensee (statutory or implied) concerning the Intellectual Property or material or derivatives, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed; (v) Institutions shall expressly disclaim any warranty regarding results obtained through the use of material or derivatives, including without limitation any claim of inaccurate, invalid or incomplete results; (vi) Institutions and their directors, trustees, officers, employees, agents, faculty, affiliated investigators and students shall have no liability to Limited Licensee, including without limitation, for any loss of use or profits, business interruption or any consequential, incidental, special or other indirect damages of any kind, regardless of how caused and regardless of whether an action in contract, tort, strict product liability or otherwise; (vii) Limited Licensee shall indemnify, defend and hold harmless the Indemnitees and HHMI Indemnitees against any liability, damage, loss or expense (including without limitation reasonable attorneys’ fees and expenses) incurred by or imposed upon any of the Indemnitees or HHMI Indemnitees, as applicable, in connection with any claims, suits, investigations, actions, demands or judgments arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of limited license by such Limited Licensee, provided that, to the extent the foregoing is not permitted by law, Limited Licensee agrees, to the extent permitted by law, that it, and not the Indemnitees or HHMI Indemnitees, as applicable, shall be responsible for any liability, damage, loss or expense arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of this limited license by Limited Licensee; and (viii) material and derivatives and their use may be the subject of one or more issued patents or pending patent applications owned by one or more Institutions, and the purchase of material does not convey a license under any claims in the foregoing patents or patent applications directed to the material or derivatives or use, production or commercialization thereof, except as expressly set forth in this limited license. Nothing in this limited license shall be construed to confer any rights upon Limited Licensee by implication, estoppel or otherwise as to any technology or patent rights of Broad or any other entity other than the Intellectual Property. In addition, nothing in this limited license shall be construed to confer upon Limited Licensee or any third party any rights under or to the Intellectual Property outside of the Customer Field.

Commercial Purposes means (a) the practice, performance or provision of any methods, process or service; or (b) the manufacture, production, sale, use, distribution, disposition or importing of any product, in each case (a) or (b) for consideration of any kind, for the purpose of sale or commercial exploitation, or on any other commercial basis.

Customer Field means use as a research tool for research purposes and use for quality control/quality assurance testing and product lot release assays, provided, however, that notwithstanding the foregoing, the Customer Field shall expressly exclude:

  1. Any human or clinical use, including, without limitation, any administration into humans or any diagnostic or prognostic use;
  2. Any human germline modification, including modifying the DNA of human embryos or human reproductive cells;
  3. Any in vivo veterinary or livestock use;
  4. The development, manufacture (including any bioproduction), distribution, importation, exportation, transportation, sale, offer for sale, marketing, promotion or other exploitation or use of the Intellectual Property or the material or derivatives as a therapeutic or diagnostic for humans or animals;
  5. Products that provide nutritional benefits and are regulated by a regulatory authority as a drug or biologic pursuant to Section 505 of the Federal Food, Drug, and Cosmetic Act of 1938, as amended, Section 351 of the Public Health Service Act of 1944, as amended, or any successor laws, or equivalent laws or regulations in jurisdiction outside the United States;
  6. Any agricultural use, including but not limited to, the use or application in the cultivation, growth, manufacture, exportation, or production of any tobacco product; and
  7. Any use or application relating to gene drive.

HHMI Indemnitees means Howard Hughes Medical Institute and its trustees, officers, employees and agents.

Indemnitees means each Institution, their affiliates, and their current and former trustees, directors, officers, faculty, affiliated investigators, students, employees, medical and professional staff and agents and their respective successors, heirs and assigns.

Institutions means each of The Broad Institute, Inc., Massachusetts Institute of Technology, Harvard College, University of Iowa Research Foundation, University of Tokyo, The Rockefeller University, New York University, New York Genome Center, and Whitehead Institute of Biomedical Research collectively.

Intellectual Property means (a) any patents and patent applications listed on Appendix A; (b) any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions of any patents or patent applications of (a), any continuations-in-part of any patents or patent applications of (a) to the extent the claims are directed to subject matter specifically described in a patent or patent application set forth on Appendix A, and any application claiming priority to a provisional application set forth on Appendix A to the extent the claims are directed to subject matter specifically described in a provisional patent application set forth on Appendix A; (c) any letters patents and/or the equivalent issuing therefrom in any jurisdiction based on, and only to the extent of, any of the foregoing; and (d) any foreign or international equivalents of any of the foregoing, including without limitation any applications filed under the Patent Cooperation Treaty.

Appendix A, Broad IP List

BROAD TECHID

APPLICANTS

EXEMPLARY FAMILY SERIAL NO

BI-2011/008

Broad, MIT, Harvard and Rockefeller

PCT/US2013/074611;

WO2014093595

BI-2011/008B

Broad, MIT and Harvard

14/259,420

BI-2011/008A

Broad and MIT

PCT/US2013/074743;

WO2014093661

BI-2011/008C

Broad, MIT, and Harvard

PCT/US2013/074790;

WO2014093694

BI-2011/020

Broad, MIT and Harvard

PCT/US2013/051418;

WO2014018423

BI-2012/084A

Broad and MIT

PCT/US2013/074825;

WO2014093718

BI-2012/084B

Broad, MIT and Harvard

PCT/US2013/074812;

WO2014093709

BI-2013/003

Broad, MIT and Harvard

PCT/US2013/074667;

WO2014093622

BI-2013/003D

Broad, MIT and Harvard

PCT/US2014/041803;

WO2014201425

BI-2013/004E

Broad, MIT and Harvard

PCT/US2013/074691;

WO2014093635

BI-2013/004F

Broad, MIT and Harvard

PCT/US2013/074736;

Wo2014093655

BI-2013/004G

Broad, MIT and Harvard

PCT/US2013/074819;

WO2014093712

BI-2013/007

Broad, MIT and Harvard

14/855,046;

US20160068822

BI-2013/066

Broad, MIT and Harvard

PCT/US2014/041800;

WO2014204724

BI-2013/073

Broad and MIT

PCT/US2014/041806;

WO2014204727

BI-2013/085

Broad, MIT and Whitehead

15/141,348;

US20160251648

BI-2013/087J

Broad, Editas*, Iowa and MIT

PCT/US2014/064663;

WO2015070083

BI-2013/087M

Broad, Iowa and MIT

PCT/US2014/069902;

WO2015089354

BI-2013/087V

Broad, Iowa and MIT

PCT/US2014/069897;

WO2015089351

BI-2013/093

Broad, MIT and Tokyo

15/171,141;

US20160340660

BI-2013/094

Broad, MIT and Rockefeller

PCT/US2014/070135;

Wo2015089465

BI-2013/098

Broad and MIT

PCT/US2014/070068; WO2015089427

BI-2013/099

Broad, MIT and Harvard

PCT/US2014/041804;

WO2014204726

BI-2013/101

Broad and MIT

PCT/US2014/070127;

WO2015089462

BI-2013/103

Broad and MIT

PCT/US2014/041808;

WO201404728

BI-2013/105

Broad, MIT and Harvard

PCT/US2014/041809;

WO2014204729

BI-2013/107

Broad and MIT

PCT/US2014/070057;

WO2015089419

BI-2013/112

Broad and MIT

PCT/US2013/074800;

WO2014093701

BI-2013/113

Broad, MIT and Harvard

PCT/US2014/070152;

WO2015089473

BI-2014/005

Broad, MIT, Harvard and Tokyo

PCT/US2014/070175;

WO2015089486

BI-2014/061

Broad, MIT and Harvard

PCT/US2015/045504;

WO2016028682

BI-2014/069

Broad and MIT

15/467,888;

US20180010134

BI-2014/071

Broad and MIT

15/349,603;

US20170107536

BI-2014/072

Broad and MIT

15/467,949;

US20180044662

BI-2014/084

Broad, MIT and Harvard

15/469,081;

US20180057810

BI-2014/097

Broad, MIT and Harvard

PCT/US2015/067177;

WO2016106244

BI-2014/100

Broad, MIT and Harvard

PCT/US2015/065385;

WO2016094867

BI-2014/101

Broad, MIT and Harvard

15/632,067;

US20170306335

BI-2014/103

Broad and MIT

PCT/US2015/067138;

WO2016100974

BI-2014/106

Broad and MIT

PCT/US2015/065393;

WO2016094872

BI-2014/107

Broad and MIT

15/619,735;

US20170349894

BI-2014/108

Broad and MIT

15/619,737;

US20170349914

BI-2014/113

Broad and MIT

15/640,103;

US20180112255

BI-2015/002

Broad, MIT, Harvard and Tokyo

PCT/US2016/038252;

WO2016205759

BI-2015/052

Broad and MIT

PCT/US2016/038034;

WO2016205613

BI-2015/053

MIT

PCT/US2016/038205;

WO2016205728

10086

Broad and MIT

PCT/US2017/047458;

WO2018035387

10114

Broad and MIT

PCT/US2017/053795;

WO2018064208

10125

Broad and MIT

62/502,064

62/564,102

10209

Broad, Harvard, MIT, New York University and NY Genome Center

62/529,573

*Broad does not purport to grant any rights in the asterisked applicant's interest in these applications in this Agreement.

BEFORE OPENING OR USING THIS PRODUCT, PLEASE READ THE TERMS AND CONDITIONS SET FORTH IN THIS LIMITED USE LABEL LICENSE. YOUR USE OF THIS PRODUCT SHALL CONSTITUTE ACKNOWLEDGMENT AND ACCEPTANCE OF THESE TERMS AND CONDITIONS. If you do not agree to use this Product pursuant to the terms and conditions set out in this Limited Use Label License, please contact Promega Corporation within ten (10) calendar days of receipt to return the unused and unopened Product for a full refund; provided, however, that custom-made Products may not be returned for a refund.

Promega is selling this Product under a research use only license from Caribou Biosciences, Inc., under certain patents and patent applications owned or controlled by Caribou. For information on obtaining rights beyond those set forth in this Limited Use Label License please contact licensing@promega.com or Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711 USA, Attn: Licensing.

The purchase of this Product conveys to you, the Buyer, the NON-TRANSFERABLE right to use this Product solely for Research Use, subject to the conditions set out in this Limited Use Label License. If you wish to use this Product for any purpose other than Research Use, you must first obtain an appropriate license. This Product may not be used for any purpose other than Research Use. Your right to use this Product for Research Use is subject to the following conditions and restrictions:

  1. Research Use means any use for internal research purposes, (e.g., for screening, characterization, quality control/quality assurance testing, product lot release assays, and/or toxicology purposes) for the sole benefit of you, the Buyer.
  2. Research Use specifically excludes the following:
    • Sale or otherwise transfer of this Product, including without limitation any material that contains this Product in whole or part or any progeny, modification or derivative of this Product (collectively, “Related Materials”) to any other third party; and
    • Commercial purposes and prohibited activities including use of this Product and Related Materials in (i) any sale, marketing, distribution, disposition, importation or exportation, whether for a fee or any other commercial basis; (ii) any commercial activity including, but not limited to, any use in manufacturing or bioproduction; (iii) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises this Product; (iv) use in a product for, or for any use in, human or animal therapeutics, diagnostics, or prophylactics; (v) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (vi) modification of the human germline, including editing human embryos or reproductive cells; (vii) stimulation of biased inheritance of a particular gene or trait or set of genes or traits; and/or (viii) use of self-administration.
  3. You, the Buyer, shall use this Product and Related Materials in compliance with all applicable laws and regulations.
  4. No warranties of any kind are provided to the you, the Buyer (statutory or implied), concerning this Product or Related Materials, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights, or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed.
  5. Your right to use this Product and Related Materials will terminate immediately if you fail to comply with these terms and conditions.

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient may genetically engineer the cell line using exogenous nucleic acid sequences provided by recipient. Recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARD TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: GA1162 Change Configuration

What's in the box?

Item Part # Size

TCRαβ-KO (CD8+) Cells (CPM)

GA116A 2 × 1ml

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

By purchase of this material, Promega Corporation (“Promega”) conveys to purchaser (the “Limited Licensee”) the non-transferable right to use the material purchased from Promega and derivatives made by Limited Licensee solely for research conducted by Limited Licensee in accordance with the following requirements: (i) Limited Licensee shall not sell or otherwise transfer material or derivatives to any other person or entity, or use material or derivatives to perform services for the benefit of any other person or entity, except to transfer the material or derivatives to a Limited Licensee affiliate provided such affiliate agrees to be bound by the terms of this Limited License; (ii) Limited Licensee shall use material and derivatives only for its internal research within the Customer Field, which may include internal research within the Customer Field in connection with product research, and not for any Commercial Purposes; (iii) Limited Licensee shall use material and derivatives in compliance with all applicable laws and regulations, including without limitation applicable human health and animal welfare laws and regulations; (iv) Institutions shall provide no warranties of any kind to Limited Licensee (statutory or implied) concerning the Intellectual Property or material or derivatives, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed; (v) Institutions shall expressly disclaim any warranty regarding results obtained through the use of material or derivatives, including without limitation any claim of inaccurate, invalid or incomplete results; (vi) Institutions and their directors, trustees, officers, employees, agents, faculty, affiliated investigators and students shall have no liability to Limited Licensee, including without limitation, for any loss of use or profits, business interruption or any consequential, incidental, special or other indirect damages of any kind, regardless of how caused and regardless of whether an action in contract, tort, strict product liability or otherwise; (vii) Limited Licensee shall indemnify, defend and hold harmless the Indemnitees and HHMI Indemnitees against any liability, damage, loss or expense (including without limitation reasonable attorneys’ fees and expenses) incurred by or imposed upon any of the Indemnitees or HHMI Indemnitees, as applicable, in connection with any claims, suits, investigations, actions, demands or judgments arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of limited license by such Limited Licensee, provided that, to the extent the foregoing is not permitted by law, Limited Licensee agrees, to the extent permitted by law, that it, and not the Indemnitees or HHMI Indemnitees, as applicable, shall be responsible for any liability, damage, loss or expense arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of this limited license by Limited Licensee; and (viii) material and derivatives and their use may be the subject of one or more issued patents or pending patent applications owned by one or more Institutions, and the purchase of material does not convey a license under any claims in the foregoing patents or patent applications directed to the material or derivatives or use, production or commercialization thereof, except as expressly set forth in this limited license. Nothing in this limited license shall be construed to confer any rights upon Limited Licensee by implication, estoppel or otherwise as to any technology or patent rights of Broad or any other entity other than the Intellectual Property. In addition, nothing in this limited license shall be construed to confer upon Limited Licensee or any third party any rights under or to the Intellectual Property outside of the Customer Field.

Commercial Purposes means (a) the practice, performance or provision of any methods, process or service; or (b) the manufacture, production, sale, use, distribution, disposition or importing of any product, in each case (a) or (b) for consideration of any kind, for the purpose of sale or commercial exploitation, or on any other commercial basis.

Customer Field means use as a research tool for research purposes and use for quality control/quality assurance testing and product lot release assays, provided, however, that notwithstanding the foregoing, the Customer Field shall expressly exclude:

  1. Any human or clinical use, including, without limitation, any administration into humans or any diagnostic or prognostic use;
  2. Any human germline modification, including modifying the DNA of human embryos or human reproductive cells;
  3. Any in vivo veterinary or livestock use;
  4. The development, manufacture (including any bioproduction), distribution, importation, exportation, transportation, sale, offer for sale, marketing, promotion or other exploitation or use of the Intellectual Property or the material or derivatives as a therapeutic or diagnostic for humans or animals;
  5. Products that provide nutritional benefits and are regulated by a regulatory authority as a drug or biologic pursuant to Section 505 of the Federal Food, Drug, and Cosmetic Act of 1938, as amended, Section 351 of the Public Health Service Act of 1944, as amended, or any successor laws, or equivalent laws or regulations in jurisdiction outside the United States;
  6. Any agricultural use, including but not limited to, the use or application in the cultivation, growth, manufacture, exportation, or production of any tobacco product; and
  7. Any use or application relating to gene drive.

HHMI Indemnitees means Howard Hughes Medical Institute and its trustees, officers, employees and agents.

Indemnitees means each Institution, their affiliates, and their current and former trustees, directors, officers, faculty, affiliated investigators, students, employees, medical and professional staff and agents and their respective successors, heirs and assigns.

Institutions means each of The Broad Institute, Inc., Massachusetts Institute of Technology, Harvard College, University of Iowa Research Foundation, University of Tokyo, The Rockefeller University, New York University, New York Genome Center, and Whitehead Institute of Biomedical Research collectively.

Intellectual Property means (a) any patents and patent applications listed on Appendix A; (b) any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions of any patents or patent applications of (a), any continuations-in-part of any patents or patent applications of (a) to the extent the claims are directed to subject matter specifically described in a patent or patent application set forth on Appendix A, and any application claiming priority to a provisional application set forth on Appendix A to the extent the claims are directed to subject matter specifically described in a provisional patent application set forth on Appendix A; (c) any letters patents and/or the equivalent issuing therefrom in any jurisdiction based on, and only to the extent of, any of the foregoing; and (d) any foreign or international equivalents of any of the foregoing, including without limitation any applications filed under the Patent Cooperation Treaty.

Appendix A, Broad IP List

BROAD TECHID

APPLICANTS

EXEMPLARY FAMILY SERIAL NO

BI-2011/008

Broad, MIT, Harvard and Rockefeller

PCT/US2013/074611;

WO2014093595

BI-2011/008B

Broad, MIT and Harvard

14/259,420

BI-2011/008A

Broad and MIT

PCT/US2013/074743;

WO2014093661

BI-2011/008C

Broad, MIT, and Harvard

PCT/US2013/074790;

WO2014093694

BI-2011/020

Broad, MIT and Harvard

PCT/US2013/051418;

WO2014018423

BI-2012/084A

Broad and MIT

PCT/US2013/074825;

WO2014093718

BI-2012/084B

Broad, MIT and Harvard

PCT/US2013/074812;

WO2014093709

BI-2013/003

Broad, MIT and Harvard

PCT/US2013/074667;

WO2014093622

BI-2013/003D

Broad, MIT and Harvard

PCT/US2014/041803;

WO2014201425

BI-2013/004E

Broad, MIT and Harvard

PCT/US2013/074691;

WO2014093635

BI-2013/004F

Broad, MIT and Harvard

PCT/US2013/074736;

Wo2014093655

BI-2013/004G

Broad, MIT and Harvard

PCT/US2013/074819;

WO2014093712

BI-2013/007

Broad, MIT and Harvard

14/855,046;

US20160068822

BI-2013/066

Broad, MIT and Harvard

PCT/US2014/041800;

WO2014204724

BI-2013/073

Broad and MIT

PCT/US2014/041806;

WO2014204727

BI-2013/085

Broad, MIT and Whitehead

15/141,348;

US20160251648

BI-2013/087J

Broad, Editas*, Iowa and MIT

PCT/US2014/064663;

WO2015070083

BI-2013/087M

Broad, Iowa and MIT

PCT/US2014/069902;

WO2015089354

BI-2013/087V

Broad, Iowa and MIT

PCT/US2014/069897;

WO2015089351

BI-2013/093

Broad, MIT and Tokyo

15/171,141;

US20160340660

BI-2013/094

Broad, MIT and Rockefeller

PCT/US2014/070135;

Wo2015089465

BI-2013/098

Broad and MIT

PCT/US2014/070068; WO2015089427

BI-2013/099

Broad, MIT and Harvard

PCT/US2014/041804;

WO2014204726

BI-2013/101

Broad and MIT

PCT/US2014/070127;

WO2015089462

BI-2013/103

Broad and MIT

PCT/US2014/041808;

WO201404728

BI-2013/105

Broad, MIT and Harvard

PCT/US2014/041809;

WO2014204729

BI-2013/107

Broad and MIT

PCT/US2014/070057;

WO2015089419

BI-2013/112

Broad and MIT

PCT/US2013/074800;

WO2014093701

BI-2013/113

Broad, MIT and Harvard

PCT/US2014/070152;

WO2015089473

BI-2014/005

Broad, MIT, Harvard and Tokyo

PCT/US2014/070175;

WO2015089486

BI-2014/061

Broad, MIT and Harvard

PCT/US2015/045504;

WO2016028682

BI-2014/069

Broad and MIT

15/467,888;

US20180010134

BI-2014/071

Broad and MIT

15/349,603;

US20170107536

BI-2014/072

Broad and MIT

15/467,949;

US20180044662

BI-2014/084

Broad, MIT and Harvard

15/469,081;

US20180057810

BI-2014/097

Broad, MIT and Harvard

PCT/US2015/067177;

WO2016106244

BI-2014/100

Broad, MIT and Harvard

PCT/US2015/065385;

WO2016094867

BI-2014/101

Broad, MIT and Harvard

15/632,067;

US20170306335

BI-2014/103

Broad and MIT

PCT/US2015/067138;

WO2016100974

BI-2014/106

Broad and MIT

PCT/US2015/065393;

WO2016094872

BI-2014/107

Broad and MIT

15/619,735;

US20170349894

BI-2014/108

Broad and MIT

15/619,737;

US20170349914

BI-2014/113

Broad and MIT

15/640,103;

US20180112255

BI-2015/002

Broad, MIT, Harvard and Tokyo

PCT/US2016/038252;

WO2016205759

BI-2015/052

Broad and MIT

PCT/US2016/038034;

WO2016205613

BI-2015/053

MIT

PCT/US2016/038205;

WO2016205728

10086

Broad and MIT

PCT/US2017/047458;

WO2018035387

10114

Broad and MIT

PCT/US2017/053795;

WO2018064208

10125

Broad and MIT

62/502,064

62/564,102

10209

Broad, Harvard, MIT, New York University and NY Genome Center

62/529,573

*Broad does not purport to grant any rights in the asterisked applicant's interest in these applications in this Agreement.

BEFORE OPENING OR USING THIS PRODUCT, PLEASE READ THE TERMS AND CONDITIONS SET FORTH IN THIS LIMITED USE LABEL LICENSE. YOUR USE OF THIS PRODUCT SHALL CONSTITUTE ACKNOWLEDGMENT AND ACCEPTANCE OF THESE TERMS AND CONDITIONS. If you do not agree to use this Product pursuant to the terms and conditions set out in this Limited Use Label License, please contact Promega Corporation within ten (10) calendar days of receipt to return the unused and unopened Product for a full refund; provided, however, that custom-made Products may not be returned for a refund.

Promega is selling this Product under a research use only license from Caribou Biosciences, Inc., under certain patents and patent applications owned or controlled by Caribou. For information on obtaining rights beyond those set forth in this Limited Use Label License please contact licensing@promega.com or Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711 USA, Attn: Licensing.

The purchase of this Product conveys to you, the Buyer, the NON-TRANSFERABLE right to use this Product solely for Research Use, subject to the conditions set out in this Limited Use Label License. If you wish to use this Product for any purpose other than Research Use, you must first obtain an appropriate license. This Product may not be used for any purpose other than Research Use. Your right to use this Product for Research Use is subject to the following conditions and restrictions:

  1. Research Use means any use for internal research purposes, (e.g., for screening, characterization, quality control/quality assurance testing, product lot release assays, and/or toxicology purposes) for the sole benefit of you, the Buyer.
  2. Research Use specifically excludes the following:
    • Sale or otherwise transfer of this Product, including without limitation any material that contains this Product in whole or part or any progeny, modification or derivative of this Product (collectively, “Related Materials”) to any other third party; and
    • Commercial purposes and prohibited activities including use of this Product and Related Materials in (i) any sale, marketing, distribution, disposition, importation or exportation, whether for a fee or any other commercial basis; (ii) any commercial activity including, but not limited to, any use in manufacturing or bioproduction; (iii) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises this Product; (iv) use in a product for, or for any use in, human or animal therapeutics, diagnostics, or prophylactics; (v) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (vi) modification of the human germline, including editing human embryos or reproductive cells; (vii) stimulation of biased inheritance of a particular gene or trait or set of genes or traits; and/or (viii) use of self-administration.
  3. You, the Buyer, shall use this Product and Related Materials in compliance with all applicable laws and regulations.
  4. No warranties of any kind are provided to the you, the Buyer (statutory or implied), concerning this Product or Related Materials, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights, or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed.
  5. Your right to use this Product and Related Materials will terminate immediately if you fail to comply with these terms and conditions.

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient may genetically engineer the cell line using exogenous nucleic acid sequences provided by recipient. Recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARD TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: GA1182 Change Configuration

What's in the box?

Item Part # Size

TCRαβ-KO (CD4+, CD8+) (CPM)

GA118A 2 × 1ml

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

By purchase of this material, Promega Corporation (“Promega”) conveys to purchaser (the “Limited Licensee”) the non-transferable right to use the material purchased from Promega and derivatives made by Limited Licensee solely for research conducted by Limited Licensee in accordance with the following requirements: (i) Limited Licensee shall not sell or otherwise transfer material or derivatives to any other person or entity, or use material or derivatives to perform services for the benefit of any other person or entity, except to transfer the material or derivatives to a Limited Licensee affiliate provided such affiliate agrees to be bound by the terms of this Limited License; (ii) Limited Licensee shall use material and derivatives only for its internal research within the Customer Field, which may include internal research within the Customer Field in connection with product research, and not for any Commercial Purposes; (iii) Limited Licensee shall use material and derivatives in compliance with all applicable laws and regulations, including without limitation applicable human health and animal welfare laws and regulations; (iv) Institutions shall provide no warranties of any kind to Limited Licensee (statutory or implied) concerning the Intellectual Property or material or derivatives, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed; (v) Institutions shall expressly disclaim any warranty regarding results obtained through the use of material or derivatives, including without limitation any claim of inaccurate, invalid or incomplete results; (vi) Institutions and their directors, trustees, officers, employees, agents, faculty, affiliated investigators and students shall have no liability to Limited Licensee, including without limitation, for any loss of use or profits, business interruption or any consequential, incidental, special or other indirect damages of any kind, regardless of how caused and regardless of whether an action in contract, tort, strict product liability or otherwise; (vii) Limited Licensee shall indemnify, defend and hold harmless the Indemnitees and HHMI Indemnitees against any liability, damage, loss or expense (including without limitation reasonable attorneys’ fees and expenses) incurred by or imposed upon any of the Indemnitees or HHMI Indemnitees, as applicable, in connection with any claims, suits, investigations, actions, demands or judgments arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of limited license by such Limited Licensee, provided that, to the extent the foregoing is not permitted by law, Limited Licensee agrees, to the extent permitted by law, that it, and not the Indemnitees or HHMI Indemnitees, as applicable, shall be responsible for any liability, damage, loss or expense arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of this limited license by Limited Licensee; and (viii) material and derivatives and their use may be the subject of one or more issued patents or pending patent applications owned by one or more Institutions, and the purchase of material does not convey a license under any claims in the foregoing patents or patent applications directed to the material or derivatives or use, production or commercialization thereof, except as expressly set forth in this limited license. Nothing in this limited license shall be construed to confer any rights upon Limited Licensee by implication, estoppel or otherwise as to any technology or patent rights of Broad or any other entity other than the Intellectual Property. In addition, nothing in this limited license shall be construed to confer upon Limited Licensee or any third party any rights under or to the Intellectual Property outside of the Customer Field.

Commercial Purposes means (a) the practice, performance or provision of any methods, process or service; or (b) the manufacture, production, sale, use, distribution, disposition or importing of any product, in each case (a) or (b) for consideration of any kind, for the purpose of sale or commercial exploitation, or on any other commercial basis.

Customer Field means use as a research tool for research purposes and use for quality control/quality assurance testing and product lot release assays, provided, however, that notwithstanding the foregoing, the Customer Field shall expressly exclude:

  1. Any human or clinical use, including, without limitation, any administration into humans or any diagnostic or prognostic use;
  2. Any human germline modification, including modifying the DNA of human embryos or human reproductive cells;
  3. Any in vivo veterinary or livestock use;
  4. The development, manufacture (including any bioproduction), distribution, importation, exportation, transportation, sale, offer for sale, marketing, promotion or other exploitation or use of the Intellectual Property or the material or derivatives as a therapeutic or diagnostic for humans or animals;
  5. Products that provide nutritional benefits and are regulated by a regulatory authority as a drug or biologic pursuant to Section 505 of the Federal Food, Drug, and Cosmetic Act of 1938, as amended, Section 351 of the Public Health Service Act of 1944, as amended, or any successor laws, or equivalent laws or regulations in jurisdiction outside the United States;
  6. Any agricultural use, including but not limited to, the use or application in the cultivation, growth, manufacture, exportation, or production of any tobacco product; and
  7. Any use or application relating to gene drive.

HHMI Indemnitees means Howard Hughes Medical Institute and its trustees, officers, employees and agents.

Indemnitees means each Institution, their affiliates, and their current and former trustees, directors, officers, faculty, affiliated investigators, students, employees, medical and professional staff and agents and their respective successors, heirs and assigns.

Institutions means each of The Broad Institute, Inc., Massachusetts Institute of Technology, Harvard College, University of Iowa Research Foundation, University of Tokyo, The Rockefeller University, New York University, New York Genome Center, and Whitehead Institute of Biomedical Research collectively.

Intellectual Property means (a) any patents and patent applications listed on Appendix A; (b) any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions of any patents or patent applications of (a), any continuations-in-part of any patents or patent applications of (a) to the extent the claims are directed to subject matter specifically described in a patent or patent application set forth on Appendix A, and any application claiming priority to a provisional application set forth on Appendix A to the extent the claims are directed to subject matter specifically described in a provisional patent application set forth on Appendix A; (c) any letters patents and/or the equivalent issuing therefrom in any jurisdiction based on, and only to the extent of, any of the foregoing; and (d) any foreign or international equivalents of any of the foregoing, including without limitation any applications filed under the Patent Cooperation Treaty.

Appendix A, Broad IP List

BROAD TECHID

APPLICANTS

EXEMPLARY FAMILY SERIAL NO

BI-2011/008

Broad, MIT, Harvard and Rockefeller

PCT/US2013/074611;

WO2014093595

BI-2011/008B

Broad, MIT and Harvard

14/259,420

BI-2011/008A

Broad and MIT

PCT/US2013/074743;

WO2014093661

BI-2011/008C

Broad, MIT, and Harvard

PCT/US2013/074790;

WO2014093694

BI-2011/020

Broad, MIT and Harvard

PCT/US2013/051418;

WO2014018423

BI-2012/084A

Broad and MIT

PCT/US2013/074825;

WO2014093718

BI-2012/084B

Broad, MIT and Harvard

PCT/US2013/074812;

WO2014093709

BI-2013/003

Broad, MIT and Harvard

PCT/US2013/074667;

WO2014093622

BI-2013/003D

Broad, MIT and Harvard

PCT/US2014/041803;

WO2014201425

BI-2013/004E

Broad, MIT and Harvard

PCT/US2013/074691;

WO2014093635

BI-2013/004F

Broad, MIT and Harvard

PCT/US2013/074736;

Wo2014093655

BI-2013/004G

Broad, MIT and Harvard

PCT/US2013/074819;

WO2014093712

BI-2013/007

Broad, MIT and Harvard

14/855,046;

US20160068822

BI-2013/066

Broad, MIT and Harvard

PCT/US2014/041800;

WO2014204724

BI-2013/073

Broad and MIT

PCT/US2014/041806;

WO2014204727

BI-2013/085

Broad, MIT and Whitehead

15/141,348;

US20160251648

BI-2013/087J

Broad, Editas*, Iowa and MIT

PCT/US2014/064663;

WO2015070083

BI-2013/087M

Broad, Iowa and MIT

PCT/US2014/069902;

WO2015089354

BI-2013/087V

Broad, Iowa and MIT

PCT/US2014/069897;

WO2015089351

BI-2013/093

Broad, MIT and Tokyo

15/171,141;

US20160340660

BI-2013/094

Broad, MIT and Rockefeller

PCT/US2014/070135;

Wo2015089465

BI-2013/098

Broad and MIT

PCT/US2014/070068; WO2015089427

BI-2013/099

Broad, MIT and Harvard

PCT/US2014/041804;

WO2014204726

BI-2013/101

Broad and MIT

PCT/US2014/070127;

WO2015089462

BI-2013/103

Broad and MIT

PCT/US2014/041808;

WO201404728

BI-2013/105

Broad, MIT and Harvard

PCT/US2014/041809;

WO2014204729

BI-2013/107

Broad and MIT

PCT/US2014/070057;

WO2015089419

BI-2013/112

Broad and MIT

PCT/US2013/074800;

WO2014093701

BI-2013/113

Broad, MIT and Harvard

PCT/US2014/070152;

WO2015089473

BI-2014/005

Broad, MIT, Harvard and Tokyo

PCT/US2014/070175;

WO2015089486

BI-2014/061

Broad, MIT and Harvard

PCT/US2015/045504;

WO2016028682

BI-2014/069

Broad and MIT

15/467,888;

US20180010134

BI-2014/071

Broad and MIT

15/349,603;

US20170107536

BI-2014/072

Broad and MIT

15/467,949;

US20180044662

BI-2014/084

Broad, MIT and Harvard

15/469,081;

US20180057810

BI-2014/097

Broad, MIT and Harvard

PCT/US2015/067177;

WO2016106244

BI-2014/100

Broad, MIT and Harvard

PCT/US2015/065385;

WO2016094867

BI-2014/101

Broad, MIT and Harvard

15/632,067;

US20170306335

BI-2014/103

Broad and MIT

PCT/US2015/067138;

WO2016100974

BI-2014/106

Broad and MIT

PCT/US2015/065393;

WO2016094872

BI-2014/107

Broad and MIT

15/619,735;

US20170349894

BI-2014/108

Broad and MIT

15/619,737;

US20170349914

BI-2014/113

Broad and MIT

15/640,103;

US20180112255

BI-2015/002

Broad, MIT, Harvard and Tokyo

PCT/US2016/038252;

WO2016205759

BI-2015/052

Broad and MIT

PCT/US2016/038034;

WO2016205613

BI-2015/053

MIT

PCT/US2016/038205;

WO2016205728

10086

Broad and MIT

PCT/US2017/047458;

WO2018035387

10114

Broad and MIT

PCT/US2017/053795;

WO2018064208

10125

Broad and MIT

62/502,064

62/564,102

10209

Broad, Harvard, MIT, New York University and NY Genome Center

62/529,573

*Broad does not purport to grant any rights in the asterisked applicant's interest in these applications in this Agreement.

BEFORE OPENING OR USING THIS PRODUCT, PLEASE READ THE TERMS AND CONDITIONS SET FORTH IN THIS LIMITED USE LABEL LICENSE. YOUR USE OF THIS PRODUCT SHALL CONSTITUTE ACKNOWLEDGMENT AND ACCEPTANCE OF THESE TERMS AND CONDITIONS. If you do not agree to use this Product pursuant to the terms and conditions set out in this Limited Use Label License, please contact Promega Corporation within ten (10) calendar days of receipt to return the unused and unopened Product for a full refund; provided, however, that custom-made Products may not be returned for a refund.

Promega is selling this Product under a research use only license from Caribou Biosciences, Inc., under certain patents and patent applications owned or controlled by Caribou. For information on obtaining rights beyond those set forth in this Limited Use Label License please contact licensing@promega.com or Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711 USA, Attn: Licensing.

The purchase of this Product conveys to you, the Buyer, the NON-TRANSFERABLE right to use this Product solely for Research Use, subject to the conditions set out in this Limited Use Label License. If you wish to use this Product for any purpose other than Research Use, you must first obtain an appropriate license. This Product may not be used for any purpose other than Research Use. Your right to use this Product for Research Use is subject to the following conditions and restrictions:

  1. Research Use means any use for internal research purposes, (e.g., for screening, characterization, quality control/quality assurance testing, product lot release assays, and/or toxicology purposes) for the sole benefit of you, the Buyer.
  2. Research Use specifically excludes the following:
    • Sale or otherwise transfer of this Product, including without limitation any material that contains this Product in whole or part or any progeny, modification or derivative of this Product (collectively, “Related Materials”) to any other third party; and
    • Commercial purposes and prohibited activities including use of this Product and Related Materials in (i) any sale, marketing, distribution, disposition, importation or exportation, whether for a fee or any other commercial basis; (ii) any commercial activity including, but not limited to, any use in manufacturing or bioproduction; (iii) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises this Product; (iv) use in a product for, or for any use in, human or animal therapeutics, diagnostics, or prophylactics; (v) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (vi) modification of the human germline, including editing human embryos or reproductive cells; (vii) stimulation of biased inheritance of a particular gene or trait or set of genes or traits; and/or (viii) use of self-administration.
  3. You, the Buyer, shall use this Product and Related Materials in compliance with all applicable laws and regulations.
  4. No warranties of any kind are provided to the you, the Buyer (statutory or implied), concerning this Product or Related Materials, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights, or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed.
  5. Your right to use this Product and Related Materials will terminate immediately if you fail to comply with these terms and conditions.

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient may genetically engineer the cell line using exogenous nucleic acid sequences provided by recipient. Recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARD TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: GA1210 Change Configuration

What's in the box?

Item Part # Size

TCRαβ-KO (CD4+) Cells (CPM)

GA117A 50 × 1ml

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

By purchase of this material, Promega Corporation (“Promega”) conveys to purchaser (the “Limited Licensee”) the non-transferable right to use the material purchased from Promega and derivatives made by Limited Licensee solely for research conducted by Limited Licensee in accordance with the following requirements: (i) Limited Licensee shall not sell or otherwise transfer material or derivatives to any other person or entity, or use material or derivatives to perform services for the benefit of any other person or entity, except to transfer the material or derivatives to a Limited Licensee affiliate provided such affiliate agrees to be bound by the terms of this Limited License; (ii) Limited Licensee shall use material and derivatives only for its internal research within the Customer Field, which may include internal research within the Customer Field in connection with product research, and not for any Commercial Purposes; (iii) Limited Licensee shall use material and derivatives in compliance with all applicable laws and regulations, including without limitation applicable human health and animal welfare laws and regulations; (iv) Institutions shall provide no warranties of any kind to Limited Licensee (statutory or implied) concerning the Intellectual Property or material or derivatives, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed; (v) Institutions shall expressly disclaim any warranty regarding results obtained through the use of material or derivatives, including without limitation any claim of inaccurate, invalid or incomplete results; (vi) Institutions and their directors, trustees, officers, employees, agents, faculty, affiliated investigators and students shall have no liability to Limited Licensee, including without limitation, for any loss of use or profits, business interruption or any consequential, incidental, special or other indirect damages of any kind, regardless of how caused and regardless of whether an action in contract, tort, strict product liability or otherwise; (vii) Limited Licensee shall indemnify, defend and hold harmless the Indemnitees and HHMI Indemnitees against any liability, damage, loss or expense (including without limitation reasonable attorneys’ fees and expenses) incurred by or imposed upon any of the Indemnitees or HHMI Indemnitees, as applicable, in connection with any claims, suits, investigations, actions, demands or judgments arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of limited license by such Limited Licensee, provided that, to the extent the foregoing is not permitted by law, Limited Licensee agrees, to the extent permitted by law, that it, and not the Indemnitees or HHMI Indemnitees, as applicable, shall be responsible for any liability, damage, loss or expense arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of this limited license by Limited Licensee; and (viii) material and derivatives and their use may be the subject of one or more issued patents or pending patent applications owned by one or more Institutions, and the purchase of material does not convey a license under any claims in the foregoing patents or patent applications directed to the material or derivatives or use, production or commercialization thereof, except as expressly set forth in this limited license. Nothing in this limited license shall be construed to confer any rights upon Limited Licensee by implication, estoppel or otherwise as to any technology or patent rights of Broad or any other entity other than the Intellectual Property. In addition, nothing in this limited license shall be construed to confer upon Limited Licensee or any third party any rights under or to the Intellectual Property outside of the Customer Field.

Commercial Purposes means (a) the practice, performance or provision of any methods, process or service; or (b) the manufacture, production, sale, use, distribution, disposition or importing of any product, in each case (a) or (b) for consideration of any kind, for the purpose of sale or commercial exploitation, or on any other commercial basis.

Customer Field means use as a research tool for research purposes and use for quality control/quality assurance testing and product lot release assays, provided, however, that notwithstanding the foregoing, the Customer Field shall expressly exclude:

  1. Any human or clinical use, including, without limitation, any administration into humans or any diagnostic or prognostic use;
  2. Any human germline modification, including modifying the DNA of human embryos or human reproductive cells;
  3. Any in vivo veterinary or livestock use;
  4. The development, manufacture (including any bioproduction), distribution, importation, exportation, transportation, sale, offer for sale, marketing, promotion or other exploitation or use of the Intellectual Property or the material or derivatives as a therapeutic or diagnostic for humans or animals;
  5. Products that provide nutritional benefits and are regulated by a regulatory authority as a drug or biologic pursuant to Section 505 of the Federal Food, Drug, and Cosmetic Act of 1938, as amended, Section 351 of the Public Health Service Act of 1944, as amended, or any successor laws, or equivalent laws or regulations in jurisdiction outside the United States;
  6. Any agricultural use, including but not limited to, the use or application in the cultivation, growth, manufacture, exportation, or production of any tobacco product; and
  7. Any use or application relating to gene drive.

HHMI Indemnitees means Howard Hughes Medical Institute and its trustees, officers, employees and agents.

Indemnitees means each Institution, their affiliates, and their current and former trustees, directors, officers, faculty, affiliated investigators, students, employees, medical and professional staff and agents and their respective successors, heirs and assigns.

Institutions means each of The Broad Institute, Inc., Massachusetts Institute of Technology, Harvard College, University of Iowa Research Foundation, University of Tokyo, The Rockefeller University, New York University, New York Genome Center, and Whitehead Institute of Biomedical Research collectively.

Intellectual Property means (a) any patents and patent applications listed on Appendix A; (b) any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions of any patents or patent applications of (a), any continuations-in-part of any patents or patent applications of (a) to the extent the claims are directed to subject matter specifically described in a patent or patent application set forth on Appendix A, and any application claiming priority to a provisional application set forth on Appendix A to the extent the claims are directed to subject matter specifically described in a provisional patent application set forth on Appendix A; (c) any letters patents and/or the equivalent issuing therefrom in any jurisdiction based on, and only to the extent of, any of the foregoing; and (d) any foreign or international equivalents of any of the foregoing, including without limitation any applications filed under the Patent Cooperation Treaty.

Appendix A, Broad IP List

BROAD TECHID

APPLICANTS

EXEMPLARY FAMILY SERIAL NO

BI-2011/008

Broad, MIT, Harvard and Rockefeller

PCT/US2013/074611;

WO2014093595

BI-2011/008B

Broad, MIT and Harvard

14/259,420

BI-2011/008A

Broad and MIT

PCT/US2013/074743;

WO2014093661

BI-2011/008C

Broad, MIT, and Harvard

PCT/US2013/074790;

WO2014093694

BI-2011/020

Broad, MIT and Harvard

PCT/US2013/051418;

WO2014018423

BI-2012/084A

Broad and MIT

PCT/US2013/074825;

WO2014093718

BI-2012/084B

Broad, MIT and Harvard

PCT/US2013/074812;

WO2014093709

BI-2013/003

Broad, MIT and Harvard

PCT/US2013/074667;

WO2014093622

BI-2013/003D

Broad, MIT and Harvard

PCT/US2014/041803;

WO2014201425

BI-2013/004E

Broad, MIT and Harvard

PCT/US2013/074691;

WO2014093635

BI-2013/004F

Broad, MIT and Harvard

PCT/US2013/074736;

Wo2014093655

BI-2013/004G

Broad, MIT and Harvard

PCT/US2013/074819;

WO2014093712

BI-2013/007

Broad, MIT and Harvard

14/855,046;

US20160068822

BI-2013/066

Broad, MIT and Harvard

PCT/US2014/041800;

WO2014204724

BI-2013/073

Broad and MIT

PCT/US2014/041806;

WO2014204727

BI-2013/085

Broad, MIT and Whitehead

15/141,348;

US20160251648

BI-2013/087J

Broad, Editas*, Iowa and MIT

PCT/US2014/064663;

WO2015070083

BI-2013/087M

Broad, Iowa and MIT

PCT/US2014/069902;

WO2015089354

BI-2013/087V

Broad, Iowa and MIT

PCT/US2014/069897;

WO2015089351

BI-2013/093

Broad, MIT and Tokyo

15/171,141;

US20160340660

BI-2013/094

Broad, MIT and Rockefeller

PCT/US2014/070135;

Wo2015089465

BI-2013/098

Broad and MIT

PCT/US2014/070068; WO2015089427

BI-2013/099

Broad, MIT and Harvard

PCT/US2014/041804;

WO2014204726

BI-2013/101

Broad and MIT

PCT/US2014/070127;

WO2015089462

BI-2013/103

Broad and MIT

PCT/US2014/041808;

WO201404728

BI-2013/105

Broad, MIT and Harvard

PCT/US2014/041809;

WO2014204729

BI-2013/107

Broad and MIT

PCT/US2014/070057;

WO2015089419

BI-2013/112

Broad and MIT

PCT/US2013/074800;

WO2014093701

BI-2013/113

Broad, MIT and Harvard

PCT/US2014/070152;

WO2015089473

BI-2014/005

Broad, MIT, Harvard and Tokyo

PCT/US2014/070175;

WO2015089486

BI-2014/061

Broad, MIT and Harvard

PCT/US2015/045504;

WO2016028682

BI-2014/069

Broad and MIT

15/467,888;

US20180010134

BI-2014/071

Broad and MIT

15/349,603;

US20170107536

BI-2014/072

Broad and MIT

15/467,949;

US20180044662

BI-2014/084

Broad, MIT and Harvard

15/469,081;

US20180057810

BI-2014/097

Broad, MIT and Harvard

PCT/US2015/067177;

WO2016106244

BI-2014/100

Broad, MIT and Harvard

PCT/US2015/065385;

WO2016094867

BI-2014/101

Broad, MIT and Harvard

15/632,067;

US20170306335

BI-2014/103

Broad and MIT

PCT/US2015/067138;

WO2016100974

BI-2014/106

Broad and MIT

PCT/US2015/065393;

WO2016094872

BI-2014/107

Broad and MIT

15/619,735;

US20170349894

BI-2014/108

Broad and MIT

15/619,737;

US20170349914

BI-2014/113

Broad and MIT

15/640,103;

US20180112255

BI-2015/002

Broad, MIT, Harvard and Tokyo

PCT/US2016/038252;

WO2016205759

BI-2015/052

Broad and MIT

PCT/US2016/038034;

WO2016205613

BI-2015/053

MIT

PCT/US2016/038205;

WO2016205728

10086

Broad and MIT

PCT/US2017/047458;

WO2018035387

10114

Broad and MIT

PCT/US2017/053795;

WO2018064208

10125

Broad and MIT

62/502,064

62/564,102

10209

Broad, Harvard, MIT, New York University and NY Genome Center

62/529,573

*Broad does not purport to grant any rights in the asterisked applicant's interest in these applications in this Agreement.

BEFORE OPENING OR USING THIS PRODUCT, PLEASE READ THE TERMS AND CONDITIONS SET FORTH IN THIS LIMITED USE LABEL LICENSE. YOUR USE OF THIS PRODUCT SHALL CONSTITUTE ACKNOWLEDGMENT AND ACCEPTANCE OF THESE TERMS AND CONDITIONS. If you do not agree to use this Product pursuant to the terms and conditions set out in this Limited Use Label License, please contact Promega Corporation within ten (10) calendar days of receipt to return the unused and unopened Product for a full refund; provided, however, that custom-made Products may not be returned for a refund.

Promega is selling this Product under a research use only license from Caribou Biosciences, Inc., under certain patents and patent applications owned or controlled by Caribou. For information on obtaining rights beyond those set forth in this Limited Use Label License please contact licensing@promega.com or Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711 USA, Attn: Licensing.

The purchase of this Product conveys to you, the Buyer, the NON-TRANSFERABLE right to use this Product solely for Research Use, subject to the conditions set out in this Limited Use Label License. If you wish to use this Product for any purpose other than Research Use, you must first obtain an appropriate license. This Product may not be used for any purpose other than Research Use. Your right to use this Product for Research Use is subject to the following conditions and restrictions:

  1. Research Use means any use for internal research purposes, (e.g., for screening, characterization, quality control/quality assurance testing, product lot release assays, and/or toxicology purposes) for the sole benefit of you, the Buyer.
  2. Research Use specifically excludes the following:
    • Sale or otherwise transfer of this Product, including without limitation any material that contains this Product in whole or part or any progeny, modification or derivative of this Product (collectively, “Related Materials”) to any other third party; and
    • Commercial purposes and prohibited activities including use of this Product and Related Materials in (i) any sale, marketing, distribution, disposition, importation or exportation, whether for a fee or any other commercial basis; (ii) any commercial activity including, but not limited to, any use in manufacturing or bioproduction; (iii) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises this Product; (iv) use in a product for, or for any use in, human or animal therapeutics, diagnostics, or prophylactics; (v) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (vi) modification of the human germline, including editing human embryos or reproductive cells; (vii) stimulation of biased inheritance of a particular gene or trait or set of genes or traits; and/or (viii) use of self-administration.
  3. You, the Buyer, shall use this Product and Related Materials in compliance with all applicable laws and regulations.
  4. No warranties of any kind are provided to the you, the Buyer (statutory or implied), concerning this Product or Related Materials, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights, or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed.
  5. Your right to use this Product and Related Materials will terminate immediately if you fail to comply with these terms and conditions.

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient may genetically engineer the cell line using exogenous nucleic acid sequences provided by recipient. Recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARD TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: GA1220 Change Configuration

What's in the box?

Item Part # Size

TCRαβ-KO (CD8+) Cells (CPM)

GA116A 50 × 1ml

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

By purchase of this material, Promega Corporation (“Promega”) conveys to purchaser (the “Limited Licensee”) the non-transferable right to use the material purchased from Promega and derivatives made by Limited Licensee solely for research conducted by Limited Licensee in accordance with the following requirements: (i) Limited Licensee shall not sell or otherwise transfer material or derivatives to any other person or entity, or use material or derivatives to perform services for the benefit of any other person or entity, except to transfer the material or derivatives to a Limited Licensee affiliate provided such affiliate agrees to be bound by the terms of this Limited License; (ii) Limited Licensee shall use material and derivatives only for its internal research within the Customer Field, which may include internal research within the Customer Field in connection with product research, and not for any Commercial Purposes; (iii) Limited Licensee shall use material and derivatives in compliance with all applicable laws and regulations, including without limitation applicable human health and animal welfare laws and regulations; (iv) Institutions shall provide no warranties of any kind to Limited Licensee (statutory or implied) concerning the Intellectual Property or material or derivatives, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed; (v) Institutions shall expressly disclaim any warranty regarding results obtained through the use of material or derivatives, including without limitation any claim of inaccurate, invalid or incomplete results; (vi) Institutions and their directors, trustees, officers, employees, agents, faculty, affiliated investigators and students shall have no liability to Limited Licensee, including without limitation, for any loss of use or profits, business interruption or any consequential, incidental, special or other indirect damages of any kind, regardless of how caused and regardless of whether an action in contract, tort, strict product liability or otherwise; (vii) Limited Licensee shall indemnify, defend and hold harmless the Indemnitees and HHMI Indemnitees against any liability, damage, loss or expense (including without limitation reasonable attorneys’ fees and expenses) incurred by or imposed upon any of the Indemnitees or HHMI Indemnitees, as applicable, in connection with any claims, suits, investigations, actions, demands or judgments arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of limited license by such Limited Licensee, provided that, to the extent the foregoing is not permitted by law, Limited Licensee agrees, to the extent permitted by law, that it, and not the Indemnitees or HHMI Indemnitees, as applicable, shall be responsible for any liability, damage, loss or expense arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of this limited license by Limited Licensee; and (viii) material and derivatives and their use may be the subject of one or more issued patents or pending patent applications owned by one or more Institutions, and the purchase of material does not convey a license under any claims in the foregoing patents or patent applications directed to the material or derivatives or use, production or commercialization thereof, except as expressly set forth in this limited license. Nothing in this limited license shall be construed to confer any rights upon Limited Licensee by implication, estoppel or otherwise as to any technology or patent rights of Broad or any other entity other than the Intellectual Property. In addition, nothing in this limited license shall be construed to confer upon Limited Licensee or any third party any rights under or to the Intellectual Property outside of the Customer Field.

Commercial Purposes means (a) the practice, performance or provision of any methods, process or service; or (b) the manufacture, production, sale, use, distribution, disposition or importing of any product, in each case (a) or (b) for consideration of any kind, for the purpose of sale or commercial exploitation, or on any other commercial basis.

Customer Field means use as a research tool for research purposes and use for quality control/quality assurance testing and product lot release assays, provided, however, that notwithstanding the foregoing, the Customer Field shall expressly exclude:

  1. Any human or clinical use, including, without limitation, any administration into humans or any diagnostic or prognostic use;
  2. Any human germline modification, including modifying the DNA of human embryos or human reproductive cells;
  3. Any in vivo veterinary or livestock use;
  4. The development, manufacture (including any bioproduction), distribution, importation, exportation, transportation, sale, offer for sale, marketing, promotion or other exploitation or use of the Intellectual Property or the material or derivatives as a therapeutic or diagnostic for humans or animals;
  5. Products that provide nutritional benefits and are regulated by a regulatory authority as a drug or biologic pursuant to Section 505 of the Federal Food, Drug, and Cosmetic Act of 1938, as amended, Section 351 of the Public Health Service Act of 1944, as amended, or any successor laws, or equivalent laws or regulations in jurisdiction outside the United States;
  6. Any agricultural use, including but not limited to, the use or application in the cultivation, growth, manufacture, exportation, or production of any tobacco product; and
  7. Any use or application relating to gene drive.

HHMI Indemnitees means Howard Hughes Medical Institute and its trustees, officers, employees and agents.

Indemnitees means each Institution, their affiliates, and their current and former trustees, directors, officers, faculty, affiliated investigators, students, employees, medical and professional staff and agents and their respective successors, heirs and assigns.

Institutions means each of The Broad Institute, Inc., Massachusetts Institute of Technology, Harvard College, University of Iowa Research Foundation, University of Tokyo, The Rockefeller University, New York University, New York Genome Center, and Whitehead Institute of Biomedical Research collectively.

Intellectual Property means (a) any patents and patent applications listed on Appendix A; (b) any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions of any patents or patent applications of (a), any continuations-in-part of any patents or patent applications of (a) to the extent the claims are directed to subject matter specifically described in a patent or patent application set forth on Appendix A, and any application claiming priority to a provisional application set forth on Appendix A to the extent the claims are directed to subject matter specifically described in a provisional patent application set forth on Appendix A; (c) any letters patents and/or the equivalent issuing therefrom in any jurisdiction based on, and only to the extent of, any of the foregoing; and (d) any foreign or international equivalents of any of the foregoing, including without limitation any applications filed under the Patent Cooperation Treaty.

Appendix A, Broad IP List

BROAD TECHID

APPLICANTS

EXEMPLARY FAMILY SERIAL NO

BI-2011/008

Broad, MIT, Harvard and Rockefeller

PCT/US2013/074611;

WO2014093595

BI-2011/008B

Broad, MIT and Harvard

14/259,420

BI-2011/008A

Broad and MIT

PCT/US2013/074743;

WO2014093661

BI-2011/008C

Broad, MIT, and Harvard

PCT/US2013/074790;

WO2014093694

BI-2011/020

Broad, MIT and Harvard

PCT/US2013/051418;

WO2014018423

BI-2012/084A

Broad and MIT

PCT/US2013/074825;

WO2014093718

BI-2012/084B

Broad, MIT and Harvard

PCT/US2013/074812;

WO2014093709

BI-2013/003

Broad, MIT and Harvard

PCT/US2013/074667;

WO2014093622

BI-2013/003D

Broad, MIT and Harvard

PCT/US2014/041803;

WO2014201425

BI-2013/004E

Broad, MIT and Harvard

PCT/US2013/074691;

WO2014093635

BI-2013/004F

Broad, MIT and Harvard

PCT/US2013/074736;

Wo2014093655

BI-2013/004G

Broad, MIT and Harvard

PCT/US2013/074819;

WO2014093712

BI-2013/007

Broad, MIT and Harvard

14/855,046;

US20160068822

BI-2013/066

Broad, MIT and Harvard

PCT/US2014/041800;

WO2014204724

BI-2013/073

Broad and MIT

PCT/US2014/041806;

WO2014204727

BI-2013/085

Broad, MIT and Whitehead

15/141,348;

US20160251648

BI-2013/087J

Broad, Editas*, Iowa and MIT

PCT/US2014/064663;

WO2015070083

BI-2013/087M

Broad, Iowa and MIT

PCT/US2014/069902;

WO2015089354

BI-2013/087V

Broad, Iowa and MIT

PCT/US2014/069897;

WO2015089351

BI-2013/093

Broad, MIT and Tokyo

15/171,141;

US20160340660

BI-2013/094

Broad, MIT and Rockefeller

PCT/US2014/070135;

Wo2015089465

BI-2013/098

Broad and MIT

PCT/US2014/070068; WO2015089427

BI-2013/099

Broad, MIT and Harvard

PCT/US2014/041804;

WO2014204726

BI-2013/101

Broad and MIT

PCT/US2014/070127;

WO2015089462

BI-2013/103

Broad and MIT

PCT/US2014/041808;

WO201404728

BI-2013/105

Broad, MIT and Harvard

PCT/US2014/041809;

WO2014204729

BI-2013/107

Broad and MIT

PCT/US2014/070057;

WO2015089419

BI-2013/112

Broad and MIT

PCT/US2013/074800;

WO2014093701

BI-2013/113

Broad, MIT and Harvard

PCT/US2014/070152;

WO2015089473

BI-2014/005

Broad, MIT, Harvard and Tokyo

PCT/US2014/070175;

WO2015089486

BI-2014/061

Broad, MIT and Harvard

PCT/US2015/045504;

WO2016028682

BI-2014/069

Broad and MIT

15/467,888;

US20180010134

BI-2014/071

Broad and MIT

15/349,603;

US20170107536

BI-2014/072

Broad and MIT

15/467,949;

US20180044662

BI-2014/084

Broad, MIT and Harvard

15/469,081;

US20180057810

BI-2014/097

Broad, MIT and Harvard

PCT/US2015/067177;

WO2016106244

BI-2014/100

Broad, MIT and Harvard

PCT/US2015/065385;

WO2016094867

BI-2014/101

Broad, MIT and Harvard

15/632,067;

US20170306335

BI-2014/103

Broad and MIT

PCT/US2015/067138;

WO2016100974

BI-2014/106

Broad and MIT

PCT/US2015/065393;

WO2016094872

BI-2014/107

Broad and MIT

15/619,735;

US20170349894

BI-2014/108

Broad and MIT

15/619,737;

US20170349914

BI-2014/113

Broad and MIT

15/640,103;

US20180112255

BI-2015/002

Broad, MIT, Harvard and Tokyo

PCT/US2016/038252;

WO2016205759

BI-2015/052

Broad and MIT

PCT/US2016/038034;

WO2016205613

BI-2015/053

MIT

PCT/US2016/038205;

WO2016205728

10086

Broad and MIT

PCT/US2017/047458;

WO2018035387

10114

Broad and MIT

PCT/US2017/053795;

WO2018064208

10125

Broad and MIT

62/502,064

62/564,102

10209

Broad, Harvard, MIT, New York University and NY Genome Center

62/529,573

*Broad does not purport to grant any rights in the asterisked applicant's interest in these applications in this Agreement.

BEFORE OPENING OR USING THIS PRODUCT, PLEASE READ THE TERMS AND CONDITIONS SET FORTH IN THIS LIMITED USE LABEL LICENSE. YOUR USE OF THIS PRODUCT SHALL CONSTITUTE ACKNOWLEDGMENT AND ACCEPTANCE OF THESE TERMS AND CONDITIONS. If you do not agree to use this Product pursuant to the terms and conditions set out in this Limited Use Label License, please contact Promega Corporation within ten (10) calendar days of receipt to return the unused and unopened Product for a full refund; provided, however, that custom-made Products may not be returned for a refund.

Promega is selling this Product under a research use only license from Caribou Biosciences, Inc., under certain patents and patent applications owned or controlled by Caribou. For information on obtaining rights beyond those set forth in this Limited Use Label License please contact licensing@promega.com or Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711 USA, Attn: Licensing.

The purchase of this Product conveys to you, the Buyer, the NON-TRANSFERABLE right to use this Product solely for Research Use, subject to the conditions set out in this Limited Use Label License. If you wish to use this Product for any purpose other than Research Use, you must first obtain an appropriate license. This Product may not be used for any purpose other than Research Use. Your right to use this Product for Research Use is subject to the following conditions and restrictions:

  1. Research Use means any use for internal research purposes, (e.g., for screening, characterization, quality control/quality assurance testing, product lot release assays, and/or toxicology purposes) for the sole benefit of you, the Buyer.
  2. Research Use specifically excludes the following:
    • Sale or otherwise transfer of this Product, including without limitation any material that contains this Product in whole or part or any progeny, modification or derivative of this Product (collectively, “Related Materials”) to any other third party; and
    • Commercial purposes and prohibited activities including use of this Product and Related Materials in (i) any sale, marketing, distribution, disposition, importation or exportation, whether for a fee or any other commercial basis; (ii) any commercial activity including, but not limited to, any use in manufacturing or bioproduction; (iii) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises this Product; (iv) use in a product for, or for any use in, human or animal therapeutics, diagnostics, or prophylactics; (v) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (vi) modification of the human germline, including editing human embryos or reproductive cells; (vii) stimulation of biased inheritance of a particular gene or trait or set of genes or traits; and/or (viii) use of self-administration.
  3. You, the Buyer, shall use this Product and Related Materials in compliance with all applicable laws and regulations.
  4. No warranties of any kind are provided to the you, the Buyer (statutory or implied), concerning this Product or Related Materials, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights, or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed.
  5. Your right to use this Product and Related Materials will terminate immediately if you fail to comply with these terms and conditions.

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient may genetically engineer the cell line using exogenous nucleic acid sequences provided by recipient. Recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARD TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Specifications

You are viewing: GA1230 Change Configuration

What's in the box?

Item Part # Size

TCRαβ-KO (CD4+, CD8+) (CPM)

GA118A 50 × 1ml

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

By purchase of this material, Promega Corporation (“Promega”) conveys to purchaser (the “Limited Licensee”) the non-transferable right to use the material purchased from Promega and derivatives made by Limited Licensee solely for research conducted by Limited Licensee in accordance with the following requirements: (i) Limited Licensee shall not sell or otherwise transfer material or derivatives to any other person or entity, or use material or derivatives to perform services for the benefit of any other person or entity, except to transfer the material or derivatives to a Limited Licensee affiliate provided such affiliate agrees to be bound by the terms of this Limited License; (ii) Limited Licensee shall use material and derivatives only for its internal research within the Customer Field, which may include internal research within the Customer Field in connection with product research, and not for any Commercial Purposes; (iii) Limited Licensee shall use material and derivatives in compliance with all applicable laws and regulations, including without limitation applicable human health and animal welfare laws and regulations; (iv) Institutions shall provide no warranties of any kind to Limited Licensee (statutory or implied) concerning the Intellectual Property or material or derivatives, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed; (v) Institutions shall expressly disclaim any warranty regarding results obtained through the use of material or derivatives, including without limitation any claim of inaccurate, invalid or incomplete results; (vi) Institutions and their directors, trustees, officers, employees, agents, faculty, affiliated investigators and students shall have no liability to Limited Licensee, including without limitation, for any loss of use or profits, business interruption or any consequential, incidental, special or other indirect damages of any kind, regardless of how caused and regardless of whether an action in contract, tort, strict product liability or otherwise; (vii) Limited Licensee shall indemnify, defend and hold harmless the Indemnitees and HHMI Indemnitees against any liability, damage, loss or expense (including without limitation reasonable attorneys’ fees and expenses) incurred by or imposed upon any of the Indemnitees or HHMI Indemnitees, as applicable, in connection with any claims, suits, investigations, actions, demands or judgments arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of limited license by such Limited Licensee, provided that, to the extent the foregoing is not permitted by law, Limited Licensee agrees, to the extent permitted by law, that it, and not the Indemnitees or HHMI Indemnitees, as applicable, shall be responsible for any liability, damage, loss or expense arising out of or related to the exercise of any rights granted to Limited Licensee under this limited license or any breach of this limited license by Limited Licensee; and (viii) material and derivatives and their use may be the subject of one or more issued patents or pending patent applications owned by one or more Institutions, and the purchase of material does not convey a license under any claims in the foregoing patents or patent applications directed to the material or derivatives or use, production or commercialization thereof, except as expressly set forth in this limited license. Nothing in this limited license shall be construed to confer any rights upon Limited Licensee by implication, estoppel or otherwise as to any technology or patent rights of Broad or any other entity other than the Intellectual Property. In addition, nothing in this limited license shall be construed to confer upon Limited Licensee or any third party any rights under or to the Intellectual Property outside of the Customer Field.

Commercial Purposes means (a) the practice, performance or provision of any methods, process or service; or (b) the manufacture, production, sale, use, distribution, disposition or importing of any product, in each case (a) or (b) for consideration of any kind, for the purpose of sale or commercial exploitation, or on any other commercial basis.

Customer Field means use as a research tool for research purposes and use for quality control/quality assurance testing and product lot release assays, provided, however, that notwithstanding the foregoing, the Customer Field shall expressly exclude:

  1. Any human or clinical use, including, without limitation, any administration into humans or any diagnostic or prognostic use;
  2. Any human germline modification, including modifying the DNA of human embryos or human reproductive cells;
  3. Any in vivo veterinary or livestock use;
  4. The development, manufacture (including any bioproduction), distribution, importation, exportation, transportation, sale, offer for sale, marketing, promotion or other exploitation or use of the Intellectual Property or the material or derivatives as a therapeutic or diagnostic for humans or animals;
  5. Products that provide nutritional benefits and are regulated by a regulatory authority as a drug or biologic pursuant to Section 505 of the Federal Food, Drug, and Cosmetic Act of 1938, as amended, Section 351 of the Public Health Service Act of 1944, as amended, or any successor laws, or equivalent laws or regulations in jurisdiction outside the United States;
  6. Any agricultural use, including but not limited to, the use or application in the cultivation, growth, manufacture, exportation, or production of any tobacco product; and
  7. Any use or application relating to gene drive.

HHMI Indemnitees means Howard Hughes Medical Institute and its trustees, officers, employees and agents.

Indemnitees means each Institution, their affiliates, and their current and former trustees, directors, officers, faculty, affiliated investigators, students, employees, medical and professional staff and agents and their respective successors, heirs and assigns.

Institutions means each of The Broad Institute, Inc., Massachusetts Institute of Technology, Harvard College, University of Iowa Research Foundation, University of Tokyo, The Rockefeller University, New York University, New York Genome Center, and Whitehead Institute of Biomedical Research collectively.

Intellectual Property means (a) any patents and patent applications listed on Appendix A; (b) any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions of any patents or patent applications of (a), any continuations-in-part of any patents or patent applications of (a) to the extent the claims are directed to subject matter specifically described in a patent or patent application set forth on Appendix A, and any application claiming priority to a provisional application set forth on Appendix A to the extent the claims are directed to subject matter specifically described in a provisional patent application set forth on Appendix A; (c) any letters patents and/or the equivalent issuing therefrom in any jurisdiction based on, and only to the extent of, any of the foregoing; and (d) any foreign or international equivalents of any of the foregoing, including without limitation any applications filed under the Patent Cooperation Treaty.

Appendix A, Broad IP List

BROAD TECHID

APPLICANTS

EXEMPLARY FAMILY SERIAL NO

BI-2011/008

Broad, MIT, Harvard and Rockefeller

PCT/US2013/074611;

WO2014093595

BI-2011/008B

Broad, MIT and Harvard

14/259,420

BI-2011/008A

Broad and MIT

PCT/US2013/074743;

WO2014093661

BI-2011/008C

Broad, MIT, and Harvard

PCT/US2013/074790;

WO2014093694

BI-2011/020

Broad, MIT and Harvard

PCT/US2013/051418;

WO2014018423

BI-2012/084A

Broad and MIT

PCT/US2013/074825;

WO2014093718

BI-2012/084B

Broad, MIT and Harvard

PCT/US2013/074812;

WO2014093709

BI-2013/003

Broad, MIT and Harvard

PCT/US2013/074667;

WO2014093622

BI-2013/003D

Broad, MIT and Harvard

PCT/US2014/041803;

WO2014201425

BI-2013/004E

Broad, MIT and Harvard

PCT/US2013/074691;

WO2014093635

BI-2013/004F

Broad, MIT and Harvard

PCT/US2013/074736;

Wo2014093655

BI-2013/004G

Broad, MIT and Harvard

PCT/US2013/074819;

WO2014093712

BI-2013/007

Broad, MIT and Harvard

14/855,046;

US20160068822

BI-2013/066

Broad, MIT and Harvard

PCT/US2014/041800;

WO2014204724

BI-2013/073

Broad and MIT

PCT/US2014/041806;

WO2014204727

BI-2013/085

Broad, MIT and Whitehead

15/141,348;

US20160251648

BI-2013/087J

Broad, Editas*, Iowa and MIT

PCT/US2014/064663;

WO2015070083

BI-2013/087M

Broad, Iowa and MIT

PCT/US2014/069902;

WO2015089354

BI-2013/087V

Broad, Iowa and MIT

PCT/US2014/069897;

WO2015089351

BI-2013/093

Broad, MIT and Tokyo

15/171,141;

US20160340660

BI-2013/094

Broad, MIT and Rockefeller

PCT/US2014/070135;

Wo2015089465

BI-2013/098

Broad and MIT

PCT/US2014/070068; WO2015089427

BI-2013/099

Broad, MIT and Harvard

PCT/US2014/041804;

WO2014204726

BI-2013/101

Broad and MIT

PCT/US2014/070127;

WO2015089462

BI-2013/103

Broad and MIT

PCT/US2014/041808;

WO201404728

BI-2013/105

Broad, MIT and Harvard

PCT/US2014/041809;

WO2014204729

BI-2013/107

Broad and MIT

PCT/US2014/070057;

WO2015089419

BI-2013/112

Broad and MIT

PCT/US2013/074800;

WO2014093701

BI-2013/113

Broad, MIT and Harvard

PCT/US2014/070152;

WO2015089473

BI-2014/005

Broad, MIT, Harvard and Tokyo

PCT/US2014/070175;

WO2015089486

BI-2014/061

Broad, MIT and Harvard

PCT/US2015/045504;

WO2016028682

BI-2014/069

Broad and MIT

15/467,888;

US20180010134

BI-2014/071

Broad and MIT

15/349,603;

US20170107536

BI-2014/072

Broad and MIT

15/467,949;

US20180044662

BI-2014/084

Broad, MIT and Harvard

15/469,081;

US20180057810

BI-2014/097

Broad, MIT and Harvard

PCT/US2015/067177;

WO2016106244

BI-2014/100

Broad, MIT and Harvard

PCT/US2015/065385;

WO2016094867

BI-2014/101

Broad, MIT and Harvard

15/632,067;

US20170306335

BI-2014/103

Broad and MIT

PCT/US2015/067138;

WO2016100974

BI-2014/106

Broad and MIT

PCT/US2015/065393;

WO2016094872

BI-2014/107

Broad and MIT

15/619,735;

US20170349894

BI-2014/108

Broad and MIT

15/619,737;

US20170349914

BI-2014/113

Broad and MIT

15/640,103;

US20180112255

BI-2015/002

Broad, MIT, Harvard and Tokyo

PCT/US2016/038252;

WO2016205759

BI-2015/052

Broad and MIT

PCT/US2016/038034;

WO2016205613

BI-2015/053

MIT

PCT/US2016/038205;

WO2016205728

10086

Broad and MIT

PCT/US2017/047458;

WO2018035387

10114

Broad and MIT

PCT/US2017/053795;

WO2018064208

10125

Broad and MIT

62/502,064

62/564,102

10209

Broad, Harvard, MIT, New York University and NY Genome Center

62/529,573

*Broad does not purport to grant any rights in the asterisked applicant's interest in these applications in this Agreement.

BEFORE OPENING OR USING THIS PRODUCT, PLEASE READ THE TERMS AND CONDITIONS SET FORTH IN THIS LIMITED USE LABEL LICENSE. YOUR USE OF THIS PRODUCT SHALL CONSTITUTE ACKNOWLEDGMENT AND ACCEPTANCE OF THESE TERMS AND CONDITIONS. If you do not agree to use this Product pursuant to the terms and conditions set out in this Limited Use Label License, please contact Promega Corporation within ten (10) calendar days of receipt to return the unused and unopened Product for a full refund; provided, however, that custom-made Products may not be returned for a refund.

Promega is selling this Product under a research use only license from Caribou Biosciences, Inc., under certain patents and patent applications owned or controlled by Caribou. For information on obtaining rights beyond those set forth in this Limited Use Label License please contact licensing@promega.com or Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711 USA, Attn: Licensing.

The purchase of this Product conveys to you, the Buyer, the NON-TRANSFERABLE right to use this Product solely for Research Use, subject to the conditions set out in this Limited Use Label License. If you wish to use this Product for any purpose other than Research Use, you must first obtain an appropriate license. This Product may not be used for any purpose other than Research Use. Your right to use this Product for Research Use is subject to the following conditions and restrictions:

  1. Research Use means any use for internal research purposes, (e.g., for screening, characterization, quality control/quality assurance testing, product lot release assays, and/or toxicology purposes) for the sole benefit of you, the Buyer.
  2. Research Use specifically excludes the following:
    • Sale or otherwise transfer of this Product, including without limitation any material that contains this Product in whole or part or any progeny, modification or derivative of this Product (collectively, “Related Materials”) to any other third party; and
    • Commercial purposes and prohibited activities including use of this Product and Related Materials in (i) any sale, marketing, distribution, disposition, importation or exportation, whether for a fee or any other commercial basis; (ii) any commercial activity including, but not limited to, any use in manufacturing or bioproduction; (iii) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises this Product; (iv) use in a product for, or for any use in, human or animal therapeutics, diagnostics, or prophylactics; (v) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (vi) modification of the human germline, including editing human embryos or reproductive cells; (vii) stimulation of biased inheritance of a particular gene or trait or set of genes or traits; and/or (viii) use of self-administration.
  3. You, the Buyer, shall use this Product and Related Materials in compliance with all applicable laws and regulations.
  4. No warranties of any kind are provided to the you, the Buyer (statutory or implied), concerning this Product or Related Materials, including without limitation, as to product quality, condition, description, merchantability, fitness for a particular purpose, noninfringement of intellectual property rights, or the absence of latent or other defects, and all such warranties are hereby expressly disclaimed.
  5. Your right to use this Product and Related Materials will terminate immediately if you fail to comply with these terms and conditions.

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use; (2) discovery, development and monitoring of biologic drugs and vaccines; (3) quality assurance testing of biologic drugs and vaccines; and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient may genetically engineer the cell line using exogenous nucleic acid sequences provided by recipient. Recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo-NL™ Luciferase Assay System purchased from Promega for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARD TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Product cannot be used for proficiency testing.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

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