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IL-15 Bioassay

A Simpler, More Consistent IL-15 Bioassay

  • Prequalified according to ICH guidelines
  • Mechanism-of-action-based assay
  • No primary cell culture required

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Catalog number selected: JA2011

$ 740.00
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IL-15 Bioassay
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Simple Bioassay, Consistent Results

Interleukin-15 (IL-15) is secreted by a variety of cell types including monocytes, macrophages, dendritic and epithelial cells. IL-15 promotes activation and expansion of natural killer (NK), natural killer T (NKT) and CD8+ memory T cells. Unlike IL-2, IL-15 doesn’t impact CD4+ regulatory T cells (Treg) or induce activation-induced cell death (AICD).

Multiple pathways can be activated by IL-15 signaling. In lymphocytes, JAK/STAT signaling begins with JAK1 and JAK3 tyrosine kinase recruitment and activation at the receptor cytoplasmic domains. These kinases recruit and activate STAT3 and 5 with phosphorylated dimer/tetramer translocation to the nucleus for transcriptional activation of a variety of proteins. These pathways impact cell proliferation, anti-apoptotic survival and cytotoxic effector functions.

The IL-15 Bioassay is a bioluminescent cell-based assay designed to measure IL-15 stimulation or inhibition using the STAT-5 response element as a readout. The IL-15 Bioassay is provided in a thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation. The IL-15 Bioassay is also available in a Cell Propagation Model format (Cat.# J2962), which includes cryopreserved cells that can be thawed, propagated and banked for long-term use.

Assay Components

The IL-15 Bioassay Cells included are provided in a thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation. 

The IL-15 Bioassay Cells have been engineered to express the response element (RE) upstream of luc2P, as well as exogenous IL-15 receptor. When IL-15 binds to IL-15 Bioassay Cells, the receptor transduces intracellular signals resulting in luminescence.The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System (Cat.# G7940) and a standard luminometer such as the GloMax® Discover System.

The Workflow

Traditional Method

12052MA-W-a

'Cells as reagents' Method

12052MA-W-b

Standardized Reagents Improve Results

  • Thaw-and-use cell format provides significant time and labor savings over traditional methods while reducing variability.
  • Kit includes all of the required reagents in standardized formats.
  • Easily amenable to high-throughput workflows.

The Assay Principle

 
15448ma
Representation of the IL-15 Bioassay. The IL-15 Bioassay consists of a genetically engineered cell line, IL-15 Bioassay Cells. When IL-15 binds to its receptor, receptor-mediated signaling induces luminescence that can be detected by adding Bio-Glo™ Reagent and quantitated with  a luminometer. In the absence of IL-15, no signaling occurs downstream of IL-15R and a luminescent signal is not generated.

Assay Performance Data

Accuracy and Precision are critical to bioassays and the development of biologics. The IL-15 Bioassay is both accurate and precise. Accuracy is a measure of how close results are to a target or reference value. Precision is a measure of repeatability. This assay was developed and prequalified according to ICH guidelines for accuracy and precision giving it the performance characteristics required for many applications of a bioassay used for antibody drug discovery research and development.

Accuracy for Assay Performance Data
Accurate, but not precise

Accuracy
Measure of ability to replicate a reference or "target" value.
IntraLab Precision
Precise, but not accurate

IntraLab Precision

Measure of repeatability from multiple assays of the same homogeneous sample under the same operating conditions over a short time (same analyst; same lab; same day).

Intermediate Precision Data Target 3
Accurate and precise

Intermediate Precision

Measure of repeatability of multiple assays of the same homogeneous sample with different analysts or equipment or on different days.

32454752-data-linear
Linear

Linearity

Ability of your assay to provide measured values that are proportional to the concentration of the sample.

Accuracy

Expected Relative Potency Recovery
50% 97.8%
70% 101.4%
140% 97.9%
200% 102.7%

Repeatability (100% reference, %CV)

1.47%

Intermediate Precision (%CV)

6.75%

Linearity

r 2 = 0.998 (50–200% range)
y = 1.03x–2.79


The IL-15 Bioassay response to recombinant IL-15.

The IL-15 Bioassay indicates stability.

15624ma
IL-15 Bioassay Cells were incubated with serial dilutions of recombinant IL-15. After a 6-hour incubation, Bio-Glo™ Reagent was added and luminescence was quantified using the GloMax® Discover System.
15451ma
Recombinant human IL-15 was heat stressed at 53°C for 0–24 hours prior to being tested in the IL-15 Bioassay. Bio-Glo™ Reagent was added and luminescence was quantified using the GloMax® Discover System.

Product Kit Formats and Related Products

  • Kits are available in 1X and 5X sizes.

Protocols

Specifications

You are viewing: JA2011 Change Configuration

What's in the box?

Item Part # Size

IL-15 Bioassay Cells

J298A 1 × 0.80ml

Fetal Bovine Serum

J121A 1 × 4ml

RPMI 1640 Medium

G708A 1 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 1 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 1 × 1 vial

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

Patent Pending.

U.S. Pat. No. 10,077,244 and other patents.

Product cannot be used for proficiency testing.

Specifications

You are viewing: JA2015 Change Configuration

What's in the box?

Item Part # Size

IL-15 Bioassay Cells

J298A 5 × 0.80ml

Fetal Bovine Serum

J121A 5 × 4ml

RPMI 1640 Medium

G708A 5 × 36ml

Bio-Glo™ Luciferase Assay Buffer

G719A 5 × 10ml

Bio-Glo™ Luciferase Assay Substrate

G720A 5 × 1 vial

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for JA2015

Search

View more results

No results

Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega’s. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

Patent Pending.

U.S. Pat. No. 10,077,244 and other patents.

Product cannot be used for proficiency testing.

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