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HaloTag® Ubiquitin Proteasome System Fusion Vectors

Fusion Vectors for the NanoBRET™ Ubiquitin Proteasome System Interaction Assays

  • Measure induced interaction between target protein and E3 ligase components VHL or CRBN with degrader compound treatment
  • Measure target protein ubiquitination
  • Measure target protein recruitment to the proteasome

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Catalog number selected: N2691

$ 500.00
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HaloTag® Ubiquitin Proteasome System Fusion Vectors
HaloTag-CRBN/20µg
$ 500.00
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Regulation of cellular protein homeostasis is critical for maintaining cell health and often altered by cellular treatments or disease states. The majority of proteins and their abundance are regulated via the ubiquitin proteasome system (UPS), which uses ubiquitin conjugation to signal proteins that should be trafficked to the proteasome for degradation. An emerging modality for small-molecule drug development is targeting proteins for degradation via the UPS with small-molecule degraders such as PROTACs or molecular glues.

The HaloTag® fusion vectors are designed to be used as the fluorescent acceptor in NanoBRET™ assays that monitor interactions of a specific target protein along the UPS pathway. NanoBRET™ technology is a proximity-based method dependent upon energy transfer from a luminescent donor target protein to a fluorescent acceptor. In these assays, the target protein fused to NanoLuc® luciferase or a subunit of NanoBiT® enzyme and serves as the bioluminescent BRET energy donor. The HaloTag® fusions are labeled with the fluorescent HaloTag® NanoBRET™ 618 Ligand and serve as the energy acceptor. When the donor and acceptor proteins interact, energy transfer occurs, resulting in increased NanoBRET™ signal.

The HaloTag®-CRBN Fusion Vector contains the coding region for an N-terminal HaloTag® fusion to the E3 ligase component Cereblon (CRBN). The vector is designed for transient or stable fusion protein expression in mammalian cells and use in a NanoBRET™ assay format to measure the ternary complex formation between a target protein, a degradation compound and CRBN in live cells. For more information see the NanoBRET™ Ternary Complex Formation Starter Kits.

HaloTag-CRBN Fusion Vector Map

HaloTag®-CRBN Fusion Vector sequence text file.

The HaloTag®-VHL Fusion Vector contains the coding region for an N-terminal HaloTag® fusion to the von Hippel-Lindau tumor suppressor E3 ligase (VHL). The vector is designed for transient or stable fusion protein expression in mammalian cells and use in a NanoBRET™ assay format to measure the ternary complex formation between a target protein, a degradation compound and VHL in live cells. For more information see the NanoBRET™ Ternary Complex Formation Starter Kits.

HaloTag-VHL Fusion Vector Map

HaloTag®-VHL Fusion Vector sequence text file.

The HaloTag®-Ubiquitin Fusion Vector contains the coding region for an N-terminal HaloTag® fusion to the ubiquitin protein (HaloTag®-UBB). The vector is designed for transient or stable expression in mammalian cells and use in a NanoBRET™ assay format to measure ubiquitination of a target protein. For more information see the NanoBRET™ Ubiquitination Assay Starter Kit.

HaloTag-Ubiquitin Fusion Vector Map

HaloTag®-Ubiquitin Fusion Vector sequence text file.

The HaloTag®-PSMD3 Fusion Vector contains the coding region for an N-terminal HaloTag® fusion to the 26S proteasome regulatory subunit 3 (PSMD3). The vector is designed for transient or stable fusion protein expression in mammalian cells and use in a NanoBRET™ assay format to measure trafficking of a target protein to the proteasome in live cells. For more information see the NanoBRET™ Proteasome Recruitment Starter Kit.

HaloTag-PSMD3 Fusion Vector Map

HaloTag®-PSMD3 Fusion Vector sequence text file.

The NanoLuc®-BRD4 FL Fusion Vector is available as a positive control for the UPS assays. When cells transfected with this vector are treated with a targeted degradation compound, the NanoBRET™ signal will increase. Use this vector to test assay functionality.

12737MA-W
NanoLuc®-BRD4 FL Vector  sequence text file.

Specifications

You are viewing: N2691 Change Configuration

What's in the box?

Item Part # SizeConcentration

HaloTag®-CRBN Fusion Vector

N269A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (194 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,425,436, 7,935,803, 8,466,269, 8,742,086, 8,420,367, 8,748,148, 9,416,353, 9,593,316 and other patents and patents pending.

Licensed from Kazusa Genome Technologies.

Specifications

You are viewing: N2701 Change Configuration

What's in the box?

Item Part # SizeConcentration

HaloTag®-PSMD3 Fusion Vector

N270A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (193 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,425,436, 7,935,803, 8,466,269, 8,742,086, 8,420,367, 8,748,148, 9,416,353, 9,593,316 and other patents and patents pending.

Licensed from Kazusa Genome Technologies.

Specifications

You are viewing: N2721 Change Configuration

What's in the box?

Item Part # SizeConcentration

HaloTag®-Ubiquitin Fusion Vector

N272A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (194 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,425,436, 7,935,803, 8,466,269, 8,742,086, 8,420,367, 8,748,148, 9,416,353, 9,593,316 and other patents and patents pending.

Specifications

You are viewing: N2731 Change Configuration

What's in the box?

Item Part # SizeConcentration

HaloTag®-VHL Fusion Vector

N273A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (192 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,425,436, 7,935,803, 8,466,269, 8,742,086, 8,420,367, 8,748,148, 9,416,353, 9,593,316 and other patents and patents pending.

Licensed from Kazusa Genome Technologies.

Specifications

You are viewing: N1691 Change Configuration

What's in the box?

Item Part # Size

NanoLuc®-BRD4 FL Fusion Vector

N169A 1 × 20μg

SDS

Choose language:

Download SDSPDF (192 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

NanoLuc® ORF TE Vector Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. No other use of this product is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product (NanoLuc® ORF Vector) for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(2a) use NanoBRET™-branded luminescent assay reagents (LARs; e.g., NanoBRET™ Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine™/Inhibitor for all determinations of luminescent activity; and
(2b) use NanoBRET™-branded energy acceptors (e.g., NanoBRET™ tracers, NanoBRET™ dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,425,436, 7,935,803, 8,466,269, 8,742,086, 8,420,367, 8,748,148, 9,416,353, 9,593,316 and other patents and patents pending.

Licensed from Kazusa Genome Technologies.

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