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TnT® Coupled Reticulocyte Lysate Systems

A Single-Tube, Coupled Transcription/Translation System

  • Study protein:protein, protein:nucleic acid and protein expression interactions
  • Generate proteins in one hour versus days
  • You choose the promoter

Choose a Promoter

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Catalog number selected: L4600

$ 670.00
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This product is discontinued
TnT® Coupled Reticulocyte Lysate Systems
SP6/40 reactions
$ 670.00
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Change Configuration

Eukaryotic Cell-Free Protein Expression

The TnT® Coupled Reticulocyte Lysate Systems offer researchers an alternative for eukaryotic cell-free protein expression: a single-tube, coupled transcription/translation system. The TnT® Lysate Systems greatly simplify the process and reduce the time required to obtain in vitro translation results. Standard rabbit reticulocyte lysate translations commonly use RNA synthesized in vitro from SP6, T3 or T7 RNA polymerase promoters and require three separate reactions with several steps between each reaction. The TnT® Systems bypass many of these steps by incorporating transcription directly in the translation mix.

For optimal protein expression using the TnT® SP6 RNA polymerase, we recommend titrating magnesium acetate in 0.1mM increments between 0.1mM and 0.5mM. In some instances the addition of 0.2mM magnesium acetate has been shown to increase protein expression by 40%. Magnesium acetate is supplied only with Cat.# L4600 and L4601.

For more information about cell-free protein expression, see the Protein Expression chapter of the Protocols & Applications Guide.

Applications

  • GST pull-downs (view an animation)
  • Gel shift assays (view an animation).
  • Co-immunoprecipitation (view an animation).
  • Characterization of protein modifications (view an animation).
  • Protein:RNA interactions.
  • Protein activity studies.
  • Confirmation of gene products.
  • Protein Truncation Test (PTT).

References

  1. Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
  2. Krieg, P.A. and Melton, D.A. (1984) Nucl. Acids Res. 12, 7057–70.

Specifications

You are viewing: L4600 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Amino Acid Mixture Minus Cysteine

L447C 1 × 50μl 1mM View Product

Luciferase Assay Wells

L457A 1 × 3 each

TnT® Reaction Buffer

L462A 1 × 90μl

TnT® SP6 Polymerase

L463A 1 × 60μl

Luciferase SP6 Control DNA

L474A 1 × 5μg 0.5μg/μl

TnT® Rabbit Reticulocyte Lysate

L483A 5 × 200μl

Luciferase Assay Reagent

L486A 1 × 250μl

Amino Acid Mixture Minus Leucine

L995A 1 × 50μl 1mM View Product

Amino Acid Mixture Minus Methionine

L996A 1 × 50μl 1mM View Product

Magnesium Acetate

L458A 1 × 100μl 25mM

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: L4601 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Luciferase Assay Wells

L457A 1 × 3 each

TnT® Reaction Buffer

L462B 1 × 20μl

TnT® SP6 Polymerase

L463C 1 × 20μl

Luciferase SP6 Control DNA

L474A 1 × 5μg 0.5μg/μl

TnT® Rabbit Reticulocyte Lysate

L483A 1 × 200μl

Luciferase Assay Reagent

L486A 1 × 250μl

Amino Acid Mixture Minus Leucine

L995D 1 × 25μl 1mM View Product

Amino Acid Mixture Minus Methionine

L996D 1 × 25μl 1mM View Product

Magnesium Acetate

L458A 1 × 100μl 25mM

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: L4610 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Amino Acid Mixture Minus Cysteine

L447C 1 × 50μl 1mM View Product

Luciferase Assay Wells

L457A 1 × 3 each

TnT® Reaction Buffer

L462A 1 × 90μl

TnT® T7 Polymerase

L481A 1 × 60μl

Luciferase T7 Control DNA

L482A 1 × 5μg 0.5μg/μl

TnT® Rabbit Reticulocyte Lysate

L483A 5 × 200μl

Luciferase Assay Reagent

L486A 1 × 250μl

Amino Acid Mixture Minus Leucine

L995A 1 × 50μl 1mM View Product

Amino Acid Mixture Minus Methionine

L996A 1 × 50μl 1mM View Product

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: L4611 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Luciferase Assay Wells

L457A 1 × 3 each

TnT® Reaction Buffer

L462B 1 × 20μl

TnT® T7 Polymerase

L481C 1 × 20μl

Luciferase T7 Control DNA

L482A 1 × 5μg 0.5μg/μl

TnT® Rabbit Reticulocyte Lysate

L483A 1 × 200μl

Luciferase Assay Reagent

L486A 1 × 250μl

Amino Acid Mixture Minus Leucine

L995D 1 × 25μl 1mM View Product

Amino Acid Mixture Minus Methionine

L996D 1 × 25μl 1mM View Product

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: L4950 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Amino Acid Mixture Minus Cysteine

L447C 1 × 50μl 1mM View Product

Luciferase Assay Wells

L457A 1 × 3 each

TnT® Reaction Buffer

L462A 1 × 90μl

TnT® Rabbit Reticulocyte Lysate

L483A 5 × 200μl

Luciferase Assay Reagent

L486A 1 × 250μl

TnT® T3 Polymerase

L493A 1 × 60μl

Luciferase T3 Control DNA

L494A 1 × 5μg 0.5μg/μl

Amino Acid Mixture Minus Leucine

L995A 1 × 50μl 1mM View Product

Amino Acid Mixture Minus Methionine

L996A 1 × 50μl 1mM View Product

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: L5010 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Amino Acid Mixture Minus Cysteine

L447C 1 × 50μl 1mM View Product

Luciferase Assay Wells

L457A 1 × 3 each

TnT® Reaction Buffer

L462A 1 × 90μl

TnT® T7 Polymerase

L481B 1 × 30μl

Luciferase T7 Control DNA

L482A 1 × 5μg 0.5μg/μl

TnT® Rabbit Reticulocyte Lysate

L483A 5 × 200μl

Luciferase Assay Reagent

L486A 1 × 250μl

TnT® T3 Polymerase

L493B 1 × 30μl

Luciferase T3 Control DNA

L494A 1 × 5μg 0.5μg/μl

Amino Acid Mixture Minus Leucine

L995A 1 × 50μl 1mM View Product

Amino Acid Mixture Minus Methionine

L996A 1 × 50μl 1mM View Product

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: L5020 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Amino Acid Mixture Minus Cysteine

L447C 1 × 50μl 1mM View Product

Luciferase Assay Wells

L457A 1 × 3 each

TnT® Reaction Buffer

L462A 1 × 90μl

TnT® SP6 Polymerase

L463B 1 × 30μl

Luciferase SP6 Control DNA

L474A 1 × 5μg 0.5μg/μl

TnT® T7 Polymerase

L481B 1 × 30μl

Luciferase T7 Control DNA

L482A 1 × 5μg 0.5μg/μl

TnT® Rabbit Reticulocyte Lysate

L483A 5 × 200μl

Luciferase Assay Reagent

L486A 1 × 250μl

Amino Acid Mixture Minus Leucine

L995A 1 × 50μl 1mM View Product

Amino Acid Mixture Minus Methionine

L996A 1 × 50μl 1mM View Product

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

 

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