pHTC HaloTag® CMV-neo Vector
Simple Multiple Cloning Site (MCS) Plasmid
- C-terminal HaloTag® Vector
- Use for traditional cloning
Catalog Number:
Size
Catalog Number: G7711
Traditional Cloning Vector
The pHTC HaloTag® CMV-neo Vector is a simple multiple cloning site (MCS) plasmid for traditional cloning. It is designed for use with other HaloTag® C-terminal vectors for expression of C-terminal-tagged HaloTag® fusion proteins in mammalian cells.
The pHTC HaloTag® CMV-neo Vector enables you to choose between a variety of initial applications (e.g., bacterial protein, mammalian, or cell-free protein expression) and then transfer to others as required. The efficient transfer allows for direct use of recombinant clones, minimizing time that can be wasted screening background colonies and is adaptable to high-throughput formats for large screening projects. With this vector there are no licensing fees or complicated transfer restrictions.
GenBank® Accession Number and Sequence Information
pHTC HaloTag® CMV-neo GenBank® Accession Number JF920305; pHTC HaloTag® CMV-neo Vector sequence text file.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
pHTC HaloTag® CMV-neo Vector |
G771A | 1 × 20μg |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,420,367, 8,748,148, 9,593,316 and 9,873,866; European Pat. Nos. 2374875, 2492342 and 2502990; and Japanese Pat. Nos. 5680302 and 5840117.
For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.
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Frequently Used With
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A 61kDa purified HaloTag®-GST fusion protein supplied at a concentration of 3.0mg/ml. Use to estimate the expression level of your HaloTag® fusion protein.
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