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Janelia Fluor® HaloTag® Ligands

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Single-Use Ligands Appropriate for High-Resolution lmaging in Live Cells and Standard Confocal Imaging

  • Rapid, single-molecule labeling
  • High signal-to-noise ratio and specificity
  • Enhanced brightness compared to fluorescent proteins

Choose a Janelia Fluor® HaloTag® Ligand

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Catalog number selected: GA1110

$ 386.00
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Janelia Fluor® HaloTag® Ligands
549/5µg
$ 386.00
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Brighter Ligands, No-Wash Protocol

The Janelia Fluor® HaloTag® Ligands enable characterization of HaloTag® fusions in endogenous cellular settings. The Janelia Fluor® dyes 525, 585 and 635 are from the same family as Janelia Fluor® 549 and 646. These dyes fill out the visible spectrum and are cell-permeable and fluorogenic; they have been used in super-resolution imaging as well as in vivo imaging.

Analogs of the Janelia Fluor® dyes (named JFX dyes) incorporate deuterium into the alkylamino substituents of rhodamines, which inhibits photochemically induced spectral shifts and slows irreparable photobleaching. The enhanced photostability of the JFX dyes enables their use in detection and single-molecule imaging studies in live cells via:

  • Super-resolution and high-resolution imaging.
  • Standard confocal imaging.
  • FACS
  • In-gel detection with a fluoroimager

Labeling of Live Cells with Janelia Fluor® 549 and Janelia Fluor® 646

Live cell imaging with fluorophore Janelia Fluor 549
Live cell labeling with Janelia Fluor 646 HaloTag Ligand 15240ta-wb
Parental U2OS cells and U2OS cells expressing HaloTag® containing a nuclear localization sequence were adhered to glass-bottom chamber slides and labeled with 200nM Janelia Fluor® 549 HaloTag® Ligand or Janelia Fluor® 646 HaloTag® Ligand for 15 minutes. Cells were imaged with 561nm laser excitation for Janelia Fluor® 549 HaloTag® Ligand (Panel A) and 637nm laser excitation for Janelia Fluor® 646 HaloTag® Ligand (Panel B). In HaloTag-expressing cells, labeling is restricted to the nucleus. Parental cells (live control in figure) show no labeling. The top row of each panel is fluorescent signal only. The bottom row shows transmitted images collected at the same respective laser excitation with the fluorescence images overlaid. Images were collected using a Nikon Eclipse Ti confocal microscope equipped with NIS Elements software and a 40X plan fluor oil immersion objective.

FACS of Janelia Fluor® HaloTag® Ligand-Labeled Proteins

Excitation-emission spectra for Janelia Fluor 549 and 646 HaloTag Ligands  15262ta-w
HEK293 cells were edited using CRISPR/Cas9 to tag endogenous BRD4 protein with HaloTag® at the N-terminus. The edited cells pool was labeled with Janelia Fluor® 646 HaloTag® Ligand and sorted on a BD FACSMelody™ sorter to isolate single clones. After expansion of cell populations, HaloTag®-expressing clones and parental HEK293 cells labeled with Janelia Fluor® 646 HaloTag® Ligand were analyzed by flow cytometry on a BD LSRFortessa™. Clonal populations expressing HaloTag fused to endogenous BRD4 (blue histogram) showed a 5-fold shift in mean fluorescence compared to labeled parental HEK293 cells (pink histogram).

In the Literature

1-ncbi-generalmethod

A general method to fine-tune fluorophores for live-cell and in vivo imaging

Learn how these new fluorescent and fluorogenic labels can open new possibilities in fluorescence microscopy in this Nature Methods paper.

Read the Paper
2-bioraxv-deuteration

Deuteration improves small-molecule fluorophores

Learn how new dyes show improved brightness, inhibit spectral shifts and slow photobleaching in this preprint from bioRxiv.

Read the Paper
3-bioraxv-optimization

Optimization and functionalization of red-shifted rhodamine dyes

Discover modified dyes for far-red or near-infrared light in this preprint from bioRxiv.

Read the Paper
50334243-halotag-ligands-catalog-page-b

Choosing the best self-labeling protein for confocal and STED microscopy

Erdmann et al. compared two self-labeling proteins, SNAP-tags and Halo-tags, in terms of expression levels, substrate permeability, target protein and dye used for labeling.

Read the Paper

Janelia Fluor® HaloTag® Ligand References

  1. Grimm, J. et al. (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nature Methods 12, 244–50.
  2. Courtney, N. et al. (2018) Excitatory and inhibitory neurons utilize different Ca2+ sensors and sources to regulate spontaneous release. Neuron 98, 977–91.
  3. Presman, D.M. et al. (2017) Quantifying transcription factor binding dynamics at the single-molecule level in live cells. Methods 123, 76–88.
  4. Guo, M. et al. (2018) Single-shot super-resolution total internal reflection fluorescence microscopy. Nature Methods 15, 425–8.
  5. Chong, S. et al. (2018) Imaging dynamic and selective low-complexity domain interactions that control gene transcription. Science DOI: 10.1126/science.aar2555

Specifications

You are viewing: GA1110 Change Configuration

What's in the box?

Item Part # Size

Janelia Fluor® 549 HaloTag® Ligand

GA111A 1 × 5μg

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 from Howard Hughes Medical Institute.

Specifications

You are viewing: GA1111 Change Configuration

What's in the box?

Item Part # Size

Janelia Fluor® 549 HaloTag® Ligand

GA111A 3 × 5μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 from Howard Hughes Medical Institute.

Specifications

You are viewing: GA1120 Change Configuration

What's in the box?

Item Part # Size

Janelia Fluor® 646 HaloTag® Ligand

GA112A 1 × 5μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 from Howard Hughes Medical Institute.

Specifications

You are viewing: GA1121 Change Configuration

What's in the box?

Item Part # Size

Janelia Fluor® 646 HaloTag® Ligand

GA112A 3 × 5μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 from Howard Hughes Medical Institute.

Specifications

You are viewing: CS315101 Change Configuration

Certificate of Analysis

Search by lot number

Storage Conditions

AA

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 from Howard Hughes Medical Institute.

Specifications

You are viewing: CS315102 Change Configuration

Certificate of Analysis

Search by lot number

Storage Conditions

AA

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 from Howard Hughes Medical Institute.

Specifications

You are viewing: CS315103 Change Configuration

Certificate of Analysis

Search by lot number

Storage Conditions

AA

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 from Howard Hughes Medical Institute.

Specifications

You are viewing: CS315104 Change Configuration

Certificate of Analysis

Search by lot number

Storage Conditions

AA

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 from Howard Hughes Medical Institute.

Specifications

You are viewing: CS315105 Change Configuration

Certificate of Analysis

Search by lot number

Storage Conditions

AA

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 from Howard Hughes Medical Institute.

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