We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Anti-LgBiT Monoclonal Antibody

N7100_Anti-LgBiT-Monoclonal-Antibody_3

Detects Large BiT (LgBiT) Subunit and LgBiT Fusion Proteins

  • Affinity-purified mouse monoclonal antibody
  • Use in Western blotting
  • Detects LgBiT subunit used in NanoBiT® complementation technology

Size

Catalog number selected: N7100

$ 349.00
Your price:
Add to Cart
This product is discontinued
Anti-LgBiT Monoclonal Antibody
100μg
$ 349.00
Your price: Log in

Detect LgBiT Subunit of NanoBiT® Complementation System

Anti-LgBiT Monoclonal Antibody is a protein A/G affinity-purified mouse monoclonal antibody that is used to detect Large BiT (LgBiT) and LgBiT fusion proteins via Western blotting. Weak cross-reactivity with NanoLuc® luciferase is observed.

We recommend a concentration of 1µg/ml as a starting point for protocol optimization.

LgBiT is the large 18kDa subunit of NanoBiT® complementation technology. LgBiT interacts with the low-affinity SmBiT or the high-affinity HiBiT peptides to form a functional luciferase. LgBiT protein fusions are used with SmBiT protein fusions for live cell analysis of protein interactions. Unfused LgBiT can also be expressed in cells to detect HiBiT-tagged proteins without the need for cell lysis.

Western Blot Results
The figure on the right shows an example Western blot using HEK293 cells transfected with CMV-based expression constructs encoding LgBiT fusion proteins. The expected mobility was seen for each of the LgBiT fusion proteins. Low levels of the LgBiT-KRAS fusion were detected even with CMV-driven expression and without dilution using Transfection Carrier DNA. 

Western blot showing detection of Large BiT fragments with Anti-LgBiT antibody.

Detection of LgBiT fusion proteins expressed in HEK293 cells.

Specifications

What's in the box?

Item Part # Size

Anti-LgBiT Monoclonal Antibody

N710A 1 × 100μg

SDS

Choose language:

Download SDSPDF (191 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-manos

Manos

UK