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Deoxyuridine Triphosphate (dUTP)

Prevent Carryover from Previous PCR Amplifications

  • Highly pure (≥99% triphosphate)
  • Supplied at 100mM in water for ease-of-use
  • Incorporate dUTP into PCR products and treat with uracil-N-glycosylase to inhibit subsequent amplifications

Size

Catalog number selected: U1191

$ 99.00
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Deoxyuridine Triphosphate (dUTP)
40μmol
$ 99.00
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High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing, cloning and genotyping. Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5.

dUTP (2´-Deoxyuridine, 5´-Triphosphate) can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is tested to ensure specific DNA amplification and the absence of nuclease activity.

  1. Longo, M.C. et al. (1990) Gene 93, 125–8.
  2. Erlich, H.A. et al. (1991) Science 252, 1643–51.

Specifications

What's in the box?

Item Part # SizeConcentration

dUTP

U119A 1 × 400μl100mM

SDS

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Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

See all certificates for U1191

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

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