Master Mix for Single-Tube RT-PCR
The AccessQuick™ RT-PCR System is an easy and convenient master mix for one-tube RT-PCR. It increases convenience by combining Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer in a single master mix. AMV RT enzyme is provided separately to allow important no-RT control reactions. The master mix is simply added to RNA templates in reaction vials, followed by AMV RT, primers and water. The AccessQuick™ RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.
- Amplify zeptomole (10–21mol) levels of RNA.
- Set up reactions in a single tube, place in the thermal cycler and come back later for results—no additions between the reverse transcription and DNA amplification steps.
- System components are stable over many freeze-thaw cycles.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.