AccessQuickâ„¢ RT-PCR System
Convenient RT-PCR in One Tube
- Mix contains Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer
- Separate AMV RT provided for important no-RT control reactions
- Simply combine AccessQuickâ„¢ Master Mix with AMV RT, RNA template, primers and water for efficient RT-PCR
Catalog Number:
Size
Catalog Number: A1701
Catalog Number: A1702
Catalog Number: A1703
Master Mix for Single-Tube RT-PCR
The AccessQuickâ„¢ RT-PCR System is an easy and convenient master mix for one-tube RT-PCR. It increases convenience by combining Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer in a single master mix. AMV RT enzyme is provided separately to allow important no-RT control reactions. The master mix is simply added to RNA templates in reaction vials, followed by AMV RT, primers and water. The AccessQuickâ„¢ RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.
- Amplify zeptomole (10–21mol) levels of RNA.
- Set up reactions in a single tube, place in the thermal cycler and come back later for results—no additions between the reverse transcription and DNA amplification steps.
- System components are stable over many freeze-thaw cycles.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
AccessQuickâ„¢ Master Mix (2X) |
A170A | 2 × 250μl | |
|
AMV Reverse Transcriptase |
A261B | 1 × 100u | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
AccessQuickâ„¢ Master Mix (2X) |
A170B | 3 × 1ml | |
|
AMV Reverse Transcriptase |
A261A | 1 × 500u | |
|
Nuclease-Free Water |
P119A | 2 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
AccessQuickâ„¢ Master Mix (2X) |
A170C | 1 × 12.5ml | |
|
AMV Reverse Transcriptase |
A261C | 1 × 2,500u | |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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