Detect SARS-CoV-2 viral RNA in preprocessed wastewater with high efficiency and sensitivity using the SARS-CoV-2 RT-qPCR Kit for Wastewater. The master mix allows sensitive detection and quantification even in the presence of reverse transcriptase inhibitors or PCR inhibitors common in wastewater samples, such as humic acids.
The kit contains a combination of primer/probe sets and controls recommended by the US Centers for Disease Control (CDC). This includes primer/probe sets targeting the N1 or N2 regions of the nucleocapsid gene, or the envelope (E) gene; primers detecting Pepper Mild Mottle Virus (PMMoV) as an internal process control; and an internal amplification control.
To provide more flexibility to test additional process controls, we also offer the PMMoV Fecal Indicator, MS2 and HF183/crAssphage RT-qPCR Kits containing master mix and appropriate primer/probe sets (without N1, N2 or E). A PMMoV Positive Control (RNA only) is available separately.