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SARS-CoV-2 RT-qPCR Kits for Wastewater

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Convenient Kits for Quantifying SARS-CoV-2 in Wastewater

  • Built-in controls and quantification standards to ensure consistent results
  • Designed for wastewater surveillance programs using CDC-recommended primer/probe sets and controls
  • Detect common SARS-CoV-2 strains with the 8-target variant panel

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Catalog number selected: AM2100

$ 900.00
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SARS-CoV-2 RT-qPCR Kits for Wastewater
N1, N2 and E/200 reactions
$ 900.00
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Sensitive Quantification of SARS-CoV-2 Viral RNA in Wastewater

Detect SARS-CoV-2 viral RNA in preprocessed wastewater with high efficiency and sensitivity using the SARS-CoV-2 RT-qPCR Kit for Wastewater. The master mix allows sensitive detection and quantification even in the presence of reverse transcriptase inhibitors or PCR inhibitors common in wastewater samples, such as humic acids.

The kits contain a combination of primer/probe sets and controls recommended by the US Centers for Disease Control (CDC). This includes primer/probe sets targeting the N1 or N2 regions of the nucleocapsid gene, or the envelope (E) gene; primers detecting Pepper Mild Mottle Virus (PMMoV) as an internal process control; and an internal amplification control.

Additional RT-qPCR kits for detecting MS2, HF183/crAssphage, norovirus, enterovirus and other pathogens are also under development. Please contact applied@promega.com for more information.

New! Detect Common SARS-CoV-2 Variants

As the SARS-CoV-2 virus continues to mutate and generate new variants of concern, it is important to monitor the spread of these variants with wastewater surveillance. The SARS-CoV-2 Variant Panel contains eight primer/probe mixes (Assays A-H) that detect mutations common to the Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617), Kappa (B.1.617.1) and Omicron (B.1.1.529) strains. The master mix (GoTaq® Enviro RT-qPCR System; AM2010) is available separately.

See how the SARS-CoV-2 Kits for Wastewater are being used in Karthikeyan, S., et al. Wastewater Sequencing Uncovers Early, Cryptic SARS-CoV-2 Variant Transmission.

Assay
Alpha, V1
(B.1.1.7)
Beta, V2
(B.1.351)
Gamma, V3 (P.1)
Delta
(B.1.617.2)
Kappa
(B.1.617.1)
Omicron
(BA.1)
Omicron
(BA.2)
Omicron
(BA.4&5)
Omicron
(BA.2.12.1)
A
S:N501Y
S:N501Y
S:N501Y
WT
WT
S:N501Y
S:N501Y
S:N501Y
S:N501Y
B
S:H69-/V70-
WT
WT
WT
WT
S:H69-/V70-
WT
S:H69-/V70-
WT
C
WT
S:K417N
No Signal
WT
WT
S:K417N
S:K417N
S:K417N
S:K417N
D
WT
No Signal
S:K417T
WT
WT
No Signal
No Signal
No Signal
No Signal
E
S:Y144-
WT
WT
WT
WT
S:Y144-
WT
WT
WT
F
WT
S:E484K
S:E484K
WT
S:E484Q
S:E484A
S:E484A
S:E484A
S:E484A
G
S:P681H
WT
WT
S:P681R
S:P681R
S:P681H
S:P681H
S:P681H
S:P681H
H
WT
WT
WT
S:L452R
S:L452R
WT
WT
S:L452R
S:L452Q
Key
Signal in FAM
Signal in HEX
No Signal,
 Other Mutation

Reference: https://covariants.org/shared-mutations

The Variant Panel kit contains sufficient material for 8 × 50 reactions (20μl RT-qPCR reaction size) or 8 × 100 reactions (10μl RT-qPCR reaction size).

How Wastewater Testing Can Help Campuses Reopen

Robert Brooks is the Technical Director and Operations Manager at Microbac Laboratories in Oak Ridge, Tennessee. Since the start of the pandemic, his lab has begun developing methods for environmental testing of the SARS-CoV-2 virus using PCR analysis. They based their PCR tests on primer sets used for clinical testing recommended by the CDC, but they had to add other components. “You can’t just take a clinical test and start running environmental samples. It doesn’t quite work like that,” says Robert. The internal controls in clinical testing kits were meant for human samples, not wastewater. To ensure accurate results, they needed a kit specifically designed for wastewater.

Learn more about how Microbac Laboratories is using the SARS-CoV-2 RT-qPCR Kit for Wastewater to develop low-cost SARS-CoV-2 surveillance testing for university campuses in this blog.

“The Promega kits include internal controls for all the different parameters that need to be measured. The separate targets give us a lot of flexibility, which is what we need."
Robert Brooks, Technical Director and Operations Manager, Microbac Laboratories

Specifications

You are viewing: AM2100 Change Configuration

What's in the box?

Item Part # Size

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 2 × 1ml

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

N1 and PMMoV Primer/Probe/IAC Mix, 20X

AM211A 1 × 100μl

N2 and PMMoV Primer/Probe/IAC Mix, 20X

AM212A 1 × 100μl

E and PMMoV Primer/Probe/IAC Mix, 20X

AM213A 1 × 100μl

Nuclease-Free Water

P119A-C 2 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: AM2110 Change Configuration

What's in the box?

Item Part # Size

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 2 × 1ml

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

N1 and PMMoV Primer/Probe/IAC Mix, 20X

AM211A 2 × 100μl

Nuclease-Free Water

P119A-C 2 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: AM2120 Change Configuration

What's in the box?

Item Part # Size

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 2 × 1ml

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

N2 and PMMoV Primer/Probe/IAC Mix, 20X

AM212A 2 × 100μl

Nuclease-Free Water

P119A-C 2 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: AM2130 Change Configuration

What's in the box?

Item Part # Size

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 2 × 1ml

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

E and PMMoV Primer/Probe/IAC Mix, 20X

AM213A 2 × 100μl

Nuclease-Free Water

P119A-C 2 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: CS3174B02 Change Configuration

Certificate of Analysis

Search by lot number

Storage Conditions

BB

Specifications

You are viewing: AM2140 Change Configuration

What's in the box?

Item Part # Size Available Separately

GoScript™ Enzyme Mix

A276A 1 × 100μl

GoTaq® Enviro Master Mix, 2X

AM200A 1 × 1ml

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

PMMoV Primer/Probe Mix, Quasar® 670

AM214A 1 × 100μl

CXR Reference Dye

C541A 1 × 100μl View Product

Nuclease-Free Water

P119A-C 1 × 1.25ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: CS317417 Change Configuration

Certificate of Analysis

Search by lot number

Storage Conditions

BB

Specifications

You are viewing: AM2070 Change Configuration

What's in the box?

Item Part # Size

PMMoV RNA, 4 × 106 copies/µl

AM207A 1 × 100μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: AM2050 Change Configuration

What's in the box?

Item Part # Size

SARS-CoV-2 (N+E) RNA, 4 × 106 copies/µl

AM205A 1 × 100μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: AM2060 Change Configuration

What's in the box?

Item Part # Size

SARS-CoV-2 (N+E) dsDNA, 2 × 106 copies/µl

AM206A 1 × 100μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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