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GoTaq® Probe 2-Step RT-qPCR System

A6110
PCR Satisfaction Guaranteed

Robust System to Simplify RNA Expression Analysis

  • Integrated components for reverse transcription and downstream qPCR
  • Compatible with both fast and standard cycling methods using probe-based assays
  • Sensitive detection for quantitation over a broad dynamic range

Size

Catalog number selected: A6110

$ 244.00 Your price: Log In

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GoTaq® Probe 2-Step RT-qPCR System
2ml
$ 244.00
Your price: Log In

Analyze Relative RNA Expression Levels

The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

Features - Benefits

  • Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
  • Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
  • Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons.
  • Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.

PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.

That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.

11723MA.png
Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 x 750μl25mM View Product

GoScript™ 5X Reaction Buffer

A500B 1 x 300μl View Product

GoScript™ Reverse Transcriptase

A501B 1 x 50μl

GoTaq® Probe qPCR Master Mix, 2X

A610A 2 x 1ml

Oligo(dT)15 Primer

C110B 1 x 50μg500μg/ml View Product

PCR Nucleotide Mix

C114G 1 x 200μl10mM

Random Primers

C118B 1 x 50μg View Product

CXR Reference Dye

C541A 1 x 100μl View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 x 2,500u

Nuclease-Free Water

P119A 3 x 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Resources

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RNA Analysis Workflow

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