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pGEM®-T Easy Vector Systems

Convenience and Flexibility for Cloning PCR Products

  • Three options for removing the insert with a single digest (BstZI, EcoRI or NotI )
  • Ligation can be completed in 1 hour at room temperature
  • Available with or without competent cells

Size

Add competent cells

Catalog number selected: A1360

$ 161.00 Your price: Log In

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pGEM®-T Easy Vector Systems
20 reactions
$ 161.00
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PCR Cloning with Blue/White Selection and Easy Insert Excision

The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the insertion site to provide a compatible overhang for PCR products. The provided 2X Rapid Ligation Buffer allows reactions to be completed in 1 hour at room temperature. The incubation period may be extended to increase the number of colonies after transformation.  

T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by Blue/White Screening on indicator plates. The vector allows preparation of single-stranded DNA due to its f1 Origin of Replication.

The pGEM®-T Easy Vector Systems offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for BstZI, EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.

The pGEM®-T Easy Vector System II contains JM109 Competent Cells in addition to all of the pGEM®-T Easy Vector System I components.

pGEM-T Easy Vector.
There is no GenBank® Accession Number for the pGEM®-T Easy Vector. The parent vector is linearized at the position indicated by * in this pGEM®-T Easy Vector Sequence and a "T" is added at each end.

Protocols

Specifications

You are viewing: A1360 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

pGEM®-T Easy Vector

A137A 1 × 1.2μg50ng/μl

Control Insert DNA

A363A 1 × 12μl

2X Rapid Ligation Buffer

C671A 1 × 200μl View Product

T4 DNA Ligase

M180A 1 × 100u

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Specifications

You are viewing: A1380 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

pGEM®-T Easy Vector

A137A 1 × 1.2μg50ng/μl

Control Insert DNA

A363A 1 × 12μl

T4 DNA Ligase

M180A 1 × 100u

2X Rapid Ligation Buffer

C671A 1 × 200μl View Product

JM109 Competent Cells, High Efficiency

L200A 6 × 200μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

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