ReliaPrep™ RNA Miniprep Systems

View information on global supply logistics

Efficiently Purify RNA from as Few as 100 Cells or 0.25mg Tissue

  • Simple, 30-minute protocol
  • Elutes in small volume, generating highly concentrated RNA
  • No phenol/chloroform or β-mercaptoethanol required

Choose a sample type

Size

Catalog number selected: Z6010

$ 119.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
ReliaPrep™ RNA Miniprep Systems
Cells/10 preps
$ 119.00
Your price: Log in
Change Configuration

Recover Concentrated, Pure RNA from Precious Cell and Tissue Samples

Precious samples, sensitive assays, low copy messages – the effectiveness of RNA purification sets the stage for downstream results.

The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes. The proprietary column/binding matrix efficiently captures RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl). 

Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or 0.25 to 20mg of tissue can be processed per purification. The system incorporates a DNase treatment step directly on the minicolumn membrane and effectively removes substances that can inhibit downstream assays. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, resulting in pure RNA that does not require additional purification or concentration of the RNA for use in demanding applications.

  • Small sample size and low elution volume allows conservation of precious samples.
  • Maximum sensitivity for downstream assays without worry of inhibition when measuring low-copy-number targets.
  • Usable, concentrated RNA is eluted in as little as 7µl. No need to further concentrate samples.
  • Rapid protocol and provided DNase reagents streamline laboratory processes.
  • Purified RNA is suitable for many common downstream assays.
Purify_RNA_Samples_439x600
RNA from Cells
RNA from Tissues

Higher RNA Yield

The data to the right show that the ReliaPrep™ Cell RNA Miniprep System efficiently isolates RNA from 100 to 1 x 106 HeLa cells with a higher RNA yield than the leading competitor.  Below, the RNA yield from 100,000 cells of 4 different cell lines. More data on performance of the ReliaPrep™ Systems is available in this Application Note.

10640MA_400x205
10485MB_400x400

Yield and Purity of RNA from Tissues

This data shows typical RNA yields from fibrous and nonfibrous mouse tissues using the ReliaPrep™ RNA Tissue Miniprep System. RNA was isolated from 10mg samples of various mouse tissues. Yield and purity were determined by absorbance spectroscopy using a NanoDrop® 1000 instrument.

More data illustrating use of the ReliaPrep™ RNA Tissue Miniprep System is available in this Application Note.

11505MA_600x565

Specifications

You are viewing: Z6010 Change Configuration

What's in the box?

Item Part # Size Available Separately

Collection Tubes

A130C 2 × 10/pk
ReliaPrep™ Minicolumns A207A 1 × 10/pack
1-Thioglycerol A208A 1 × 75μl

Column Wash Solution (CWE)

A254A 1 × 1ml

Nuclease-Free Water

P119A 1 × 1,250μl View Product

BL Buffer

Z103A 2 × 3.25ml

Elution Tubes

Z200B 1 × 25/pk

RNA Wash Solution (RWA)

Z309B 1 × 11.8ml View Product
Yellow Core Buffer Z317B 1 × 300μl

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358B 1 × 1 vial View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: Z6011 Change Configuration

What's in the box?

Item Part # Size Available Separately

Collection Tubes

A130A 2 × 50/pk

ReliaPrep™ Minicolumns

A207B 1 × 50/pack

1-Thioglycerol

A208B 1 × 900μl

Column Wash Solution (CWE)

A254B 1 × 5ml

Nuclease-Free Water

P119E 1 × 13ml View Product

BL Buffer

Z103B 1 × 32.5ml

Elution Tubes

Z200C 1 × 50/pk
RNA Wash Solution (RWA) Z309E 1 × 35ml View Product

Yellow Core Buffer

Z317A 1 × 2.5ml

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358A 1 × 1 vial View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: Z6012 Change Configuration

What's in the box?

Item Part # Size Available Separately

Collection Tubes

A130B 2 × 250/pk

ReliaPrep™ Minicolumns

A207B 5 × 50/pack

1-Thioglycerol

A208B 2 × 900μl

Column Wash Solution (CWE)

A254C 1 × 24ml

Nuclease-Free Water

P119E 1 × 13ml View Product
BL Buffer Z103C 1 × 150ml

Elution Tubes

Z200C 5 × 50/pk

RNA Wash Solution (RWA)

Z309D 1 × 206ml View Product

Yellow Core Buffer

Z317C 1 × 11ml

MnCl2, 0.09M

Z318B 2 × 750μl

DNase I (lyophilized)

Z358A 3 × 1 vial View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: Z6110 Change Configuration

What's in the box?

Item Part # Size Available Separately

Collection Tubes

A130C 2 × 10/pk
ReliaPrep™ Minicolumns A207A 1 × 10/pack
1-Thioglycerol A208A 2 × 75μl

Column Wash Solution (CWE)

A254A 1 × 1ml

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Lysis Buffer (LBA)

Z106A 1 × 6ml

Elution Tubes

Z200B 1 × 25/pk

RNA Wash Solution (RWA)

Z309B 1 × 11.8ml View Product
Yellow Core Buffer Z317B 1 × 300μl

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358B 1 × 1 vial View Product

RNA Dilution Buffer (RDB)

Z372C 1 × 9ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: Z6111 Change Configuration

What's in the box?

Item Part # Size Available Separately

Collection Tubes

A130A 2 × 50/pk

ReliaPrep™ Minicolumns

A207B 1 × 50/pack

1-Thioglycerol

A208B 2 × 900μl

Column Wash Solution (CWE)

A254B 1 × 5ml

Nuclease-Free Water

P119E 1 × 13ml View Product

Lysis Buffer (LBA)

Z106B 1 × 30ml

Elution Tubes

Z200C 1 × 50/pk
RNA Wash Solution (RWA) Z309E 1 × 35ml View Product

Yellow Core Buffer

Z317A 1 × 2.5ml

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358A 1 × 1 vial View Product

RNA Dilution Buffer (RDB)

Z372A 1 × 44ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: Z6112 Change Configuration

What's in the box?

Item Part # Size Available Separately

Collection Tubes

A130B 2 × 250/pk

ReliaPrep™ Minicolumns

A207B 5 × 50/pack

1-Thioglycerol

A208B 4 × 900μl

Column Wash Solution (CWE)

A254C 1 × 24ml

Nuclease-Free Water

P119E 1 × 13ml View Product

Lysis Buffer (LBA)

Z106C 1 × 150ml

Elution Tubes

Z200C 5 × 50/pk

RNA Wash Solution (RWA)

Z309D 1 × 206ml View Product

Yellow Core Buffer

Z317C 1 × 11ml

MnCl2, 0.09M

Z318B 2 × 750μl

DNase I (lyophilized)

Z358A 3 × 1 vial View Product

RNA Dilution Buffer (RDB)

Z372B 1 × 220ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-angus

Angus

UK