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Maxwell® 16 Tissue LEV Total RNA Purification Kit

Maxwell 16 Tissue LEV Total RNA Purif Kit 48 preps
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Maxwell® 16 Tissue LEV Total RNA Purification Kit

Purify RNA from 1–16 Samples in 45 Minutes

The Maxwell® 16 Tissue LEV Total RNA Purification Kit is used with the Maxwell® 16 LEV Instrument. This RNA purification procedure provides an easy method for efficient, automated purification of highly concentrated total RNA from up to 25mg of tissue and 2.5ml of whole blood collected into PAXgene® tubes. Purified RNA for up to 16 samples is obtained in less than 45 minutes of hands-free instrument operation. No post-purification treatment with nuclease, cleanup or concentration is required to achieve superior performance in downstream applications. The low elution volume (30–100μl) is used to generate purified RNA in a more concentrated format for use in downstream applications such as qRT-PCR, RT-PCR and cDNA synthesis.

  • Essentially undetectable contaminating genomic DNA means fewer repeated experiments and unexplained or variable results.
  • For most sample types, eluted RNA concentration is directly ready for qRT-PCR. No post-purification concentration is required.
  • High yields and high-concentration total RNA result in better performance in gene expression analysis applications.
No detectable genomic DNA contamination.

RNA was isolated from 24 separate samples of 25mg of mouse liver lysate and analyzed by endpoint and qRT-PCR. Of the 24 individual RNA isolations, the average contaminating gDNA content was demonstrated to be 0.1 genome equivalent per 100ng of Maxwell®-16 purified RNA.

Specifications

What's in the box?

Item Part # Size Available Separately

Collection Tubes

A130A 1 × 50/pk

LEV Plungers

AS610A 1 × 50/pk View Product

Elution Tubes (0.5ml)

AS620A 1 × 50/pk View Product

Maxwell® 16 LEV RNA Cartridge

AS640A 48 × 1 each

Nuclease-Free Water

P119C 1 × 25ml View Product

RNA Lysis Buffer (RLA)

Z3051 1 × 50ml View Product

RNA Dilution Buffer (RDB)

Z372A 1 × 44ml

Clearing Agent (CAA)

Z373D 1 × 6.5ml

Beta-Mercaptoethanol

Z559A 1 × 900μl

Clearing Columns

Z568A 1 × 50/pk

Certificate of Analysis

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

DD

Resources

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