EXO-NET® Pan-Exosome Capture and Maxwell® HT miRNA Extraction Kits

Exosomes are small extracellular vesicles, ranging in size from 30–150 nanometers. Released by a variety of cell types, exosomes play a crucial role in intercellular communication. These vesicles carry an array of molecules, including proteins, lipids and nucleic acids that reflect an exosome's cell of origin and can influence the behavior of recipient cells. Exosome research is dynamic and includes understanding their basic biology, developing detection and quantification techniques, and exploring their use in regenerative medicine and cancer therapy. Purifying exosomes previously required time-consuming manual methods.

The EXO-NET® Pan Exosome Capture Tool automates exosome isolation from a variety of biological samples. Using a unique capture mechanism, this tool helps researchers efficiently isolate exosomes with a high degree of purity and integrity. These are critical parameters for subsequent analyses in biomarker research, cellular communication studies and disease pathogenesis investigations.

Detection of RNA purified from exosomes isolated from plasma. Exosomes were isolated from 200µl of plasma from 3 individuals in duplicate according to the method described in the protocol. Following exosome isolation, RNA was purified from exosomes in 200µl of TBS according to the method described in the protocol. Purified RNA was amplified via 2-step RT-qPCR with TaqMan® assays targeting miR21 and let7a using the TaqMan® MicroRNA Reverse Transcription Kit and GoTaq® Probe qPCR Master Mix (Cat.# A6101). Purified RNA was also amplified via 1-step RT-qPCR with primers targeting β2M using GoTaq® 1-Step RT-qPCR System (Cat.# A6120). Average C_q value and standard deviation of duplicate purifications amplified in duplicate are shown. Each target was amplified separately, and C_q values cannot be compared across targets.