Lumit® IgG Titer Immunoassay
Measure IgG Antibody Titers Across Your Development Workflow
- Versatile: Suitable for multiple IgG subclasses and antibody isotypes
- Simple: “Add-mix-read” protocol with no wash steps
- No immobilization to plates, beads or other surfaces required
- Assays available for Human, Mouse and Rabbit antibodies
Lumit® Human IgG Titer Immunoassays
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3716A07 | Early Access Lumit® Human IgG Titer Immunoassay | View Specifications | 100 assays | Please Enquire | |||
| CS3716A13 | Early Access Lumit® Human IgG Titer Immunoassay | View Specifications | 1000 assays | Please Enquire |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Lumit® Mouse IgG Titer Immunoassays
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3716A03 | Early Access Lumit® Mouse IgG Titer Immunoassay | View Specifications | 100 assays | Please Enquire | |||
| CS3716A10 | Early Access Lumit® Mouse IgG Titer Immunoassay | View Specifications | 1000 assays | Please Enquire |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Lumit® Rabbit IgG Titer Immunoassays
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3716A09 | Early Access Lumit® Rabbit IgG Titer Immunoassay | View Specifications | 100 assays | Please Enquire | |||
| CS3716A14 | Early Access Lumit® Rabbit IgG Titer Immunoassay | View Specifications | 1000 assays | Please Enquire |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Easily Quantify Antibody Titers at Every Step
During antibody therapeutics development, accurately tracking antibody concentration—or titer—at each stage is critical; it informs candidate selection, ensures consistency during process scaleup and predicts in vivo efficacy and safety profiles. Variations in titer can mask suboptimal clones, lead to costly batch failures or delay regulatory submission; yet traditional assays are often labor-intensive, prone to inter-operator variability and constrained by limited dynamic range.
The Lumit® IgG Titer Immunoassay streamlines this process with a high-throughput assay that delivers precise, reproducible measurements—from early discovery screening and clone selection through optimization of upstream expression and downstream purification. By combining sensitive detection chemistry with easy-to-interpret data, Lumit® IgG Titer Immunoassay empowers researchers to confidently compare candidates, troubleshoot process deviations and accelerate their path to the clinic.
Efficient, Sensitive and Accurate Antibody Titers
Principle of Lumit® IgG Titer Immunoassay. Detection of IgG antibodies is performed by the binding of NanoBiT®-labeled specific antibodies (i.e., Lumit® Anti-Human IgG Antibody-LgBiT and Lumit® Anti-Human IgG Antibody-SmBiT). Binding of the antibodies to the IgG promotes the reconstitution of NanoLuc® luciferase, generating bioluminescence in the presence of its substrate, furimazine.
Assay Workflow: Versatile Antibody Detection
- Broad applicability across antibody development stages
- User-friendly protocol for quicker enablement
- Easily amenable to high-throughput workflows
Lumit® IgG Titer Immunoassay Detects A Diversity of Antibodies
Lumit® Human IgG Titer Immunoassay detects multiple subclasses and isotypes. The Lumit® Human IgG Titer Assay can quantify (Panel A) all IgG subclasses and (Panel B) other human antibody isotypes. After incubation, the Lumit® Detection Reagent was added and luminescence determined in a GloMax® Discover Plate Reader.
IgG Titer technology works with a variety of non-human antibodies, including rabbit and mouse.
Are you working with additional species?
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Anti-Human Ab-SmBiT |
CS3716A05 | 1 × 50μl | |
Human Antibody (IgG1) Standard |
CS3716A06 | 1 × 50μl | 5000ng/ml |
Lumit® Detection Substrate A |
VB301D | 1 × 75μl | |
Lumit® Immunoassay Dilution Buffer A |
CS3716A01 | 1 × 1ml | |
Lumit® Anti-Human Ab-LgBiT |
CS3716A04 | 1 × 50μl |
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Human Antibody (IgG1) Standard |
CS3716A06 | 2 × 50μl | 5000ng/ml |
Lumit® Anti-Human Ab-LgBiT |
CS3716A11 | 1 × 500μl | |
Lumit® Anti-Human Ab-SmBiT |
CS3716A12 | 1 × 500μl | |
Lumit® Immunoassay Dilution Buffer A, 10X |
VB201A | 1 × 10ml | |
Lumit® Detection Substrate A |
VB301D | 4 × 75μl |
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Immunoassay Dilution Buffer A |
CS3716A01 | 1 × 1ml | |
Mouse Antibody (IgG1) Standard |
CS3716A02 | 1 × 50μl | 5000ng/ml |
Lumit® Detection Substrate A |
VB301D | 1 × 75μl | |
Lumit® Anti-Mouse Ab-LgBiT |
W102A | 1 × 30μl | |
Lumit® Anti-Mouse Ab-SmBiT |
W105A | 1 × 30μl |
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Mouse Antibody (IgG1) Standard |
CS3716A02 | 2 × 50μl | 5000ng/ml |
Lumit® Immunoassay Dilution Buffer A, 10X |
VB201A | 1 × 10ml | |
Lumit® Detection Substrate A |
VB301D | 4 × 75μl | |
Lumit® Anti-Mouse Ab-LgBiT |
W102B | 1 × 300μl | |
Lumit® Anti-Mouse Ab-SmBiT |
W105B | 1 × 300μl |
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Lumit® Immunoassay Dilution Buffer A |
CS3716A01 | 1 × 1ml | |
Rabbit Antibody (IgG1) Standard |
CS3716A08 | 1 × 50μl | 5000ng/ml |
Lumit® Detection Substrate A |
VB301D | 1 × 75μl | |
Lumit® Anti-Rabbit Ab-SmBiT |
W103A | 1 × 30μl | |
Lumit® Anti-Rabbit Ab-LgBiT |
W104A | 1 × 30μl |
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
Rabbit Antibody (IgG1) Standard |
CS3716A08 | 2 × 50μl | 5000ng/ml |
Lumit® Immunoassay Dilution Buffer A, 10X |
VB201A | 1 × 10ml | |
Lumit® Detection Substrate A |
VB301D | 4 × 75μl | |
Lumit® Anti-Rabbit Ab-SmBiT |
W103B | 1 × 300μl | |
Lumit® Anti-Rabbit Ab-LgBiT |
W104B | 1 × 300μl |
Resources
Featured Resource
Scientific Poster: The Lumit® IgG Titer Immunoassay can quantify all IgG subclasses and other immunoglobulin isotypes. Combined with the specific Ig standard, the Lumit® IgG Titer Immunoassay is a universal immunoglobulin quantification tool.
- Efficient and High-Throughput Quantification: Run in as little as 20 minutes.
- Enhanced Sensitivity and Dynamic Range: Detection limits as low as 0.4ng/ml.
- Broad Applicability Across mAb Development Stages: Suitable for discovery, manufacturing and quality control settings.
Related Products
Similar Products
Lumit® Anti-Tag Protein Interaction Reagents
Homogeneous assay to measure protein-protein or protein-small molecule interactions involving tagged proteins.
W1600, W1601, W1611, W1620, W1621, W1631, W1640, W1641, W1651, W1660, W1661, W1671, CS332214, CS332222, CS332223
Lumit® IFN-γ (Human) Immunoassay
Quantitatively measures released IFN-γ in cell culture samples using a simple, no-wash protocol.
W6040, W6042, W6041, W134A-C
Lumit® Immunoassay Labeling Kit and Detection Reagents
Antibody labeling kit and detection reagents to help develop Lumit® Immunoassays.
VB2500, VB2010, VB2020, VB2030, VB4050, VB4060
Lumit® Immunoassays for Detection of Metabolic Regulators
Assays to detect insulin or glucagon in cell culture samples with a scalable, no-wash protocol.
Frequently Used With
Lumit® FcγR Binding Immunoassays
Novel homogeneous competition assays to measure the interaction between human Fc receptors and antibodies or Fc fusion proteins.
W7030, W7031, W7040, W7041, W7050, W7051, W7060, W7061, W7070, W7071, W7080, W7081
ADCC Reporter Bioassay, V Variant
Measure potency and stability of antibodies mediating ADCC through the high-affinity human FcγRIIIa-V receptor.
G7015, G7014, G7010, G7016, G7013, G7018, G7102, GA1130
ADCP Reporter Bioassay (THP-1)
Measure the potency and stability of antibodies and other biologics with Fc domains that bind and activate FcγRs.
JA9411, JA9415, GA1272, GA6020
PD-1/PD-L1 Blockade Bioassay
Measure the ability of biologics to block immune checkpoint signals
J1250, J1255, J1252, J1191, J1195, J1201
