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PowerSeq® Whole Mito System

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Streamlined Workflow for Complete Mitochondrial Genome MPS Analysis

  • Massively parallel sequencing of whole mitochondrial genome in two amplification reactions
  • More efficient workflow greatly reduces number of steps and time required for library preparation
  • Improved results with compromised samples—generates 161 small amplicons

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Catalog number selected: PS1600

$ 7,400.00
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PowerSeq® Whole Mito System
100 samples
$ 7,400.00
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Streamlined Workflow for mtDNA Analysis

Massively parallel sequencing (MPS) allows laboratories access to mitochondrial DNA (mtDNA) analysis using a more sensitive and high-throughput workflow compared to traditional sequencing methods. Increased mixture deconvolution and heteroplasmy resolution are achieved by deep sequencing coverage and digital read counts. Additionally, the use of small amplicons to sequence the mitochondrial control region improves sequencing results from degraded samples.

The PowerSeq® Whole Mito System generates 161 small amplicons covering the entire region of the mitochondrial genome. The targeted regions for amplification are designed for an average size of 167bp to ensure optimal results from degraded samples. The system contains reagents for library preparation and a streamlined workflow that greatly reduces the number of steps and time required to produce libraries ready for sequencing.

Sequencing data can be analyzed using GeneMarker HTS Software (SoftGenetics, LLC).

powerseq whole mito system workflow

PowerSeq® Whole Mito System workflow.

coverage across libraries generated with the powerseq whole mito system

Percent major variant, minimum and average coverage across the whole mitochondrial genome. Varying input amounts of positive control 2800M DNA were amplified and sequenced using PowerSeq® Whole Mito System and MiSeq® sequencing reagents and analyzed using GeneMarker® HTS software, version 2.5. Each concentration was sequenced in duplicate, and 22 libraries were pooled in a single run. 

PowerSeq® Whole Mito System Components

The PowerSeq® Whole Mito System is comprised of two kits, the PowerSeq® Whole Mito Amp and Prep Kit (Cat.# PS1650) and the PowerSeq® Whole Mito Purification Kit (Cat.# PS2010).

PowerSeq® Whole Mito Amp and Prep Kit (Cat.# PS1650)

Cat.# PS1650 provides sufficient reagents to perform 100 amplification reactions.

Item Part# Size Available Separately
Pre-Amplification Box
PowerSeq® Whole Mito 5X Master Mix DP407A 1 × 500µl
PowerSeq® Whole Mito 10X Primer Mix 1 DK482A 1 × 250µl
PowerSeq® Whole Mito 10X Primer Mix 2 DK483A 1 × 250µl
Water, Amplification Grade DW099A 2 × 1,250µl View Product
2800M Control DNA, 10ng/µl DD710A 1 × 25µl View Product
Post-Amplification Box
Library Prep Reagent PS210A 1 × 400µl
Library Prep Buffer PS220A 6 × 500µl

 

PowerSeq® Whole Mito Purification Kit (Cat.# PS2010)

Cat.# PS2010 provides sufficient reagents to perform 100 purifications.

Item Part# Size Available Separately
ProNex® Size-Selective Chemistry
NG103C 1 × 25ml
ProNex® Binding Buffer NG102A 1 × 4ml
Wash Buffer
NG105C 1 × 40ml
Elution Buffer
NG116C 1 × 15ml
Nuclease-Free Water P119L 1 × 7ml View Product
Proteinase K (PK) Solution MC500D 1 × 1ml View Product

 

Specifications

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

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