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PowerSeq™ 46GY System

Combined Autosomal STR and Y-STR loci in a single MPS library workflow

  • Targeted panels of familiar loci ensure an expected outcome for data analysis
  • Largest combination of autosomal loci and Y-STRs loci in a single kit
  • Improved mixture interpretation results due to better amplicon balance within STRs

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PowerSeq™ 46GY System

An Optimal MPS Workflow


The PowerSeq™ 46GY System contains small amplicons (109-303bp) which can be used to prepare MPS libraries and generate sequencing data compatible with Illumina TruSeq™ and MiSeq™ technologies. With this workflow, both sequence and allele length polymorphism in the autosomal and Y-STR loci can be identified. The large number of alleles increases the statistical power of inclusion and decreases the frequency of overlapping alleles.

The combination of these STR loci and Amelogenin makes this multiplex an effective tool for human identification using MPS and maintains compatibility with existing databases worldwide. By offering a combination of familiar markers, The PowerSeq™ 46GY offers you a smooth transition to include MPS into your workflow, ensuring you get the data you want to use.


The PowerSeq™ 46GY System integrates into your laboratory workflow without the burden of analyzing supplementary, unestablished markers—making your transition to MPS a bit easier. When using the PowerSeq™ Systems, you can expect to see similar performance with regards to sensitivity, inhibitor tolerance and balance that you've come to expect from our PowerPlex® STR Systems line.

PS4600 PowerSeq 46GY 14948ma-w

Reliable Sensitivity


The PowerSeq™ 46GY System offers the same sensitivity that you have come to depend on when using our PowerPlex® systems. Full profiles can be obtained with as little as 32.5pg of input DNA. As shown in the figure below, the balance at low template inputs is comparable to the balance seen at the recommended input amount of 500pg.


PS4600 PowerSeq 46GY Sensitivity 14929ma-w
Percent of total allele calls per locus at different amounts of 2800M Control DNA (n=3). A pool of 44 samples was sequenced simultaneously using MiSeq® Reagent Kit v3, 2x300 cycles. Data were analyzed with STRait Razor (University of North Texas Health Science Center).

Large Combination of Dependable Loci


The PowerSeq™ 46GY System has the largest combination of autosomal and Y-STR loci in a single kit to generate sequence data and improve your MPS workflow. Amplicons for each locus are designed to be in a range of 140–300bp. The loci included are CODIS core and European Standard Set (ESS) loci, Amelogenin and the Y-STR loci included in the PowerPlex® Y23 System.

View Loci
Locus Name
D8S1179
D21S11
D7S820
CSF1PO
D3S1358
TH01
D13S317
D16S539
D2S1338
D19S433
vWA
TPOX
D18S51
D5S818
FGA
Penta D
Penta E
Amelogenin
D1S1656
D2S441
D10S1248
D12S391
D22S1045
DYS391
Locus Name
DYS391
DYS19
DYS385ab
DYS389I/II
DYS390
DYS392
DYS393
DYS437
DYS438
DYS439
DYS448
DYS456
DYS458
DYS481
DYS533
DYS549
DYS570
DYS576
DYS635
DYS643
Y-GATA-H4

Improved Analysis of DNA Mixtures


The evaluation and interpretation of complex forensic DNA mixtures continues to challenge forensic laboratories. Improved balance between loci and the ability to identify minor contributor alleles based on length and sequence polymorphism greatly helps with mixture interpretation.


Locus
1:1
Female:Male Ratio
2:1
Female:Male Ratio
5:1
Female:Male Ratio
9:1
Female:Male Ratio
19:1
Female:Male Ratio
D1S1656
1.3
2.4
5.5
12.4
27
D21S11
1.3
2.5
6.7
8.3
13
D8S1179
1.2
2.3
6.7
12.9
14,19,62
TPOX
1.5
2.7
7.3
13
22,23,52
Several female:male mixtures were prepared in the following ratios: 1:1, 2:1, 5:1, 9:1, 19:1 where a 1:1 mixture contained 250pg female DNA and 250pg male DNA and 19:1 mixture contained 475pg female DNA and 25pg male DNA (a total input amount of 500pg of DNA) and amplified in triplicate. The data was checked against the expected genotypes and all female and male genotypes were detected at each mixture concentration. The ratios were calculated at the loci with unique alleles for the two contributors. While there is a larger spread in two of the loci in the 19:1 ratio, the data is consistent with the expected ratios.

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