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The ILS included with the 5-dye PowerPlex® Systems has changed. What are the changes?

The appropriate use of an ILS during capillary electrophoresis allows automated data analysis and correct DNA fragment size assessments between runs. As part of our effort to provide products that exceed your performance expectations, we have included an ILS with WEN, a dye which is brighter and more photostable and can be stored at 4°C, in our PowerPlex® Fusion 6C System. Recently, we have changed the dye used in the ILS in our PowerPlex® 5-dye STR systems as well. The new ILS has the same fragment sizes as the current CC5 ILS 500 fragments, but the fragments are instead labeled with WEN dye.


How does the ILS change affect analysis of PowerPlex® 5-dye STR data?

Fragments labeled with CC5 and WEN migrate slightly differently during capillary electrophoresis, and thus this dye change will require the use of updated panels and bins for correct allele assignment in unknown samples. Also, the slight migration differences will result in changes in predicted sizes of artifacts seen with various 5-dye PowerPlex® Systems.

The figure below demonstrates that although the fragments migrate differently, the allele calls are concordant and correct.

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Allelic ladder samples were mixed with either 1–2µl of CC5 ILS500 or 0.5µl of WEN ILS 500 and then injected on an Applied Biosystems® 3500xL Genetic Analyzer with a 1.2kV, 24-second injection. Ladders were analyzed with a 175RFU analysis threshold, and data were analyzed with GeneMapper® ID-X software using the current or updated panels and bins text files.

Does this mean the matrix standards have also changed to include the WEN dye?

Yes, due to the ILS dye change, a corresponding dye change has been implemented in our matrix kits. The matrix standards for multicapillary instruments are now provided in a single-tube format with a recommended storage temperature of 4°C—making it more convenient for users.


What changed in the PowerPlex® STR kits?

The pre-amplification components of the STR system (primer mix and master mix) have not changed. The catalog number and product names of the STR kits also have not changed. The only change is that beginning June 10, 2016, the 5-dye PowerPlex® Systems will contain an ILS that is labeled with WEN dye instead of CC5 dye.


How do I choose the right replacement product for evaluation?

The table below highlights the key changes for the ILS associated with PowerPlex® 5-dye STR chemistry. Also included is the first batch information for the PowerPlex® System kits that contain WEN ILS 500 products.

PowerPlex® System Name Catalog Number First Batch #s containing WEN ILS 500 Component Changes

PowerPlex® 18D System

DC1802
DC1808
0000204862
0000204866
WEN ILS 500 (Part# DG500A) replaces CC5 ILS 500 (Part# DG152A).

PowerPlex® 21 System

DC8902
DC8942
0000206125

PowerPlex® Fusion System

DC2402
DC2408
0000202523
0000202524

PowerPlex® ESX 17 System

DC6720
0000201497
0000201504

PowerPlex® ESI 17 Pro System

DC7780
0000203426
0000203427
WEN ILS 500 ESS (Part# DG510A) replaces CC5 ILS 500 Pro (Part# DG348A).

PowerPlex® ESX 17 System and PowerPlex® ESI 17 Pro System Bundle

DC6794   WEN ILS 500 (Part# DG500A) replaces CC5 ILS 500 (Part# DG152A),
and WEN ILS 500 ESS (Part# DG510A) replaces CC5 ILS 500 Pro (Part# DG348A).

PowerPlex® ESX 16 Fast System

DC1610
DC1611
0000201434
0000201395
WEN ILS 500 ESS (Part# DG510A) replaces CC5 ILS 500 Pro (Part# DG348A).

PowerPlex® ESI 16 Fast System

DC1620
DC1621
0000207278
0000207280

PowerPlex® ESX 17 Fast System

DC1710
DC1711
0000205251
0000205249

PowerPlex® ESI 17 Fast System

DC1721
DC1720
0000201443
0000201453

PowerPlex® ESX/ESI 16 Fast System Bundle

DC1631
DC1630
 

PowerPlex® ESX/ESI 17 Fast System Bundle

DC1731
DC1730
 

PowerPlex® Y23 System

DC2305
DC2320
0000206127
0000206131
WEN ILS 500 Y23 (Part# DG520A) replaces CC5 ILS 500 Y23 (Part# DG380A).
What kind of testing should I perform prior to adopting WEN ILS in my workflow?

You should determine the best course of action for an internal evaluation needed prior to adopting WEN ILS in your workflow. For your convenience, we have provided an example of an appropriate evaluation plan below.

13292MA
Please contact Technical Services with any additional questions.
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