MyGlo Instrument now $4950. Use code GETMYGLO at checkout to get 10% off MyGlo. Shop Now ›

Malate-Glo™ Assay

customize-this-small
View information on global supply logistics

Easy, Sensitive L-Malate Assay Kit

  • Compatible with cells, tissue and serum samples
  • In-well sample preparation protocol streamlines workflow
  • Scalable to 384-well plates for HTS applications

Size

Catalog number selected: JE9100

$ 475.00
Your price:
Add to Cart
This product is discontinued
Request a Sample
Not available in all countries
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Malate-Glo™ Assay
5ml
$ 475.00
Your price: Log in
Change Configuration

Efficient Detection of Malate in Diverse Samples

The Malate-Glo™ Assay is a fast and sensitive method for detecting L-malate from various biological samples, such as cultured cells, tissue homogenates and serum. Based on bioluminescent technology, the Malate-Glo™ Assay can detect subtle changes in mitochondrial metabolism and the TCA cycle. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation and spin columns. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.

How the Malate-Glo™ Assay Works

Diagram illustrating how the Malate-Glo™ Assay works.

Malate Dehydrogenase catalyzes the oxidation of malate with concomitant reduction of NAD+ to NADH. In the presence of NADH, Reductase enzymatically reduces the proluciferin Reductase Substrate to luciferin. Luciferin is detected using Ultra-Glo™ Recombinant Luciferase, and the amount of light produced is proportional to the amount of malate in the sample.

Simple Add-and-Read Protocol Saves Time

Diagram illustrating the Malate-Glo™ Assay protocol workflow.

Accurately Detect the Smallest Changes in Malate

Graph showing the linear relationship between luminescence and malate concentration.


Sensitivity (S/B >5)

400nM

Limit of Detection (S/N >3)

50nM

Linear Range

50nM to 25μM

Maximum Assay Window (S/B)

>200

Sensitive Quantitation of Malate in Cultured Cells




Cellular malate content varies depending on cell type, growth conditions and mitochondrial function.
The Malate-Glo™ Assay, with its high sensitivity and wide linear detection range, measures malate levels in cell lysate samples using 390–25,000 cells per 50μl.

Graph showing the linear luminescent signal for malate concentration in a range of HCT116 cell cultures.

Do you want to learn more about our cellular energy metabolism tools and the innovative technology that powers these assays?

Specifications

You are viewing: JE9100 Change Configuration

What's in the box?

Item Part # Size Concentration

Malate Dehydrogenase

J408A 1 × 25μl

Malate, 10mM

J409A 1 × 50μl

Reductase

G884A 1 × 55μl 6mg/ml

Reductase Substrate

G885A 1 × 55μl

Luciferin Detection Solution

J135A 1 × 5ml

NAD

J136B 1 × 275μl

Neutralization Buffer

J153A 1 × 15ml

NADP+

J154B 1 × 100μl

0.6N HCl

J402A 1 × 15ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Specifications

You are viewing: JE9200 Change Configuration

What's in the box?

Item Part # Size Concentration

Malate Dehydrogenase

J408B 1 × 250μl

Malate, 10mM

J409A 1 × 50μl

Reductase

G884B 1 × 275μl 6mg/ml

Reductase Substrate

G885B 1 × 275μl

Luciferin Detection Solution

J135B 1 × 50ml

NAD

J136C 1 × 1ml

Neutralization Buffer

J153A 1 × 15ml

NADP+

J154C 1 × 500μl

0.6N HCl

J402A 1 × 15ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Let's find the product that meets your needs.

Talk to a Scientist

Maria

Maria

Spain