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Wizard® Genomic DNA Purification Kit

Manual, Solution-Based DNA Extraction Method

  • DNA from blood, animal and plant cells, bacteria or yeast
  • 60-minute protocol
  • Scale volumes to the amount of material processed

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Catalog number selected: A1120

$ 173.00 Your price: Log In

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Wizard® Genomic DNA Purification Kit
100 isolations × 300μl
$ 173.00
Your price: Log In
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Simple Method to Isolate Genomic DNA from Many Sample Types

The Wizard® Genomic DNA Purification Kit provides a simple, solution-based method for isolation of DNA from white blood cells, tissue culture cells, animal tissue, plant tissue, yeast and Gram-positive and Gram-negative bacteria. DNA purified with this system is suitable for a variety of applications, including amplification, digestion with restriction endonucleases and membrane hybridizations (e.g., Southern and dot/slot blots).

Learn more about our custom options for this product at: www.promega.com/custom/

Sample Types Processed with the Wizard® Genomic Kit

Blood
Sample TypePromegaExternalComments
Blood and blood cells
Human whole blood
Yes
Yes
Protocols are provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050 for samples of up to 10ml blood or in 96-well plates ("Isolating Genomic DNA from Whole Blood" sections). The protocol works with the following anticoagulants: EDTA, heparin and citrate.
Reference:
Rizzo, W.B., Carney, G. and Lin, Z. (1999) Am. J. Hum. Genet. 65, 1547–60. PubMed ID #10577908
Mouse whole blood
Yes
A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050.
Human peripheral blood mononuclear cells
Yes
Tan, A. et al. (2006) Clin. Chem. 52, 2250–7.
PubMed ID #17040960
Bovine whole blood
Yes
Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348
Horse whole blood
Yes
Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348
Sheep whole blood
Yes
Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348
Antelope whole blood
Yes
Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348
Dog whole blood
Yes
Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348
Cat whole blood
Yes
Walker, J.A. et al.(2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348
Rabbit whole blood
Yes
Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348
Mammalian Tissues
Sample TypePromegaExternalComments
Mammalian tissues
Mouse liver
Yes
Yes
Tissue needs to be homogenized prior to isolation. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section.
Reference:
Barazzoni, R. et al. (2005) Endocrinology 146, 2098–106.
PubMed ID #15618355.
Mouse tail
Yes
Yes
Tail needs to be treated with proteinase K before purification. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section.
Reference:
Nielsen, J.V. et al. (2007) Development 134, 1133–40.
PubMed ID #17301088.
Mouse brain
Yes
Sinz, E.H. et al. (1999) J. Clin. Invest. 104, 647–56.
PubMed ID #10487779.
Mouse Lymphoma
Yes
Yu, Z. et al. (2006) Cancer Res. 66, 755–62.
PubMed ID #16424006.
Rat skeletal muscle, liver and heart
Yes
Barazzoni, R. et al. (2000) J. Biol. Chem. 275, 3343–7.
PubMed ID #10652323.
Human bone marrow aspirate
Yes
Yu, Z. et al. (2006) Cancer Res. 66, 755–62.
PubMed ID #16424006.
Human liver and testicle
Yes
Maduro, M.R. et al. (2003) Mol. Hum. Reprod. 9, 61–8.
PubMed ID #12569174.
Human colon
Yes
Tost, J. et al. (2003) Nucleic Acids Res. 31, e50.
PubMed ID #12711695.
Cultured Cells
Sample TypePromegaExternalComments
Plant tissues
Tomatoe leaf
Yes
Leaf needs to be frozen in liquid nitrogen and ground with a mortar and pestle prior to adding lysis buffer. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Plant Tissue" section.
Arabidopsis
Yes
Maize
Yes
Lemna minor (aquatic plant)
Yes
Stout, L.M. and Nüsslein, K. (2005) Appl. Environ. Microbiol. 71, 2484–92.
PubMed ID #15870338.
Medicago truncatula
Yes
Total DNA was isolated from roots, which may include various mycorrhizal fungi.
Kosuta, S. et al. (2003) Plant Physiol. 131, 952–62.
PubMed ID #12644648.
Tetrahymena thermophila
Yes
Followed plant DNA protocol ("Isolating Genomic DNA from Plant Tissue"), but skipped freezing in liquid nitrogen and grinding with a mortar and pestle.
Jacobs, M.E. et al. (2006) Eukaryot. Cell 5, 1990–2000.
PubMed ID #17012537.
Bacteria
Sample TypePromegaExternalComments
Cultured cells (Established cell lines are not listed.)
Yes
Yes
A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section.
Reference:
Zhang, S. et al. (2006) Cancer Res. 66, 8847–57.
PubMed ID #16951202.
Drosophila D.Mel-2 embryonic cells
Yes
Roberti, M. et al. (2006) Nucleic Acids Res. 34, 2109–16.
PubMed ID #16648357.
Mouse embryonic stem cells
Yes
Oka, M. et al. (2006) J. Biol. Chem. 281, 9901–8.
PubMed ID #16439359.
Monkey primary fibroblasts, virus-infected
Yes
Cells were lysed using 300 µl of Nuclei Lysis Solution with proteinase K and incubating overnight at 37°C.
Mansfield, K.G. et al. (1999) J. Virol. 73, 10320–8.
PubMed ID #10559350.
Vero cells infected with HSV-1
Yes
Both viral and genomic DNA were isolated.
Bower, J.R. et al. (1999) J. Virol. 73, 3843–53.
PubMed ID #10196279.
CD45RA+ T cells from cord blood
Yes
Samples of 2–4 × 106 cells were pelleted, washed with PBS, snap frozen in liquid nitrogen and frozen at –70°C prior to using the Wizard® Genomic DNA Purification Kit. Soares et al. (1998) J. Immunol. 161, 5909–17.
PubMed ID #9834071.
Bacteria – Gram Negative
Sample TypePromegaExternalComments
Bacteria – Gram Negative
Enterobacter cloacae
Yes
A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section.
Escherichia coli
Yes
Yes
A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section.
Reference:
Zheng, W. et al. (2001) EMBO J. 20, 1164–72.
PubMed ID #11230139.
Nitrobacter winogradskyi
Yes
Starkenburg, S.R. et al. (2006) Appl. Environ. Microbiol. 72, 2050–63.
PubMed ID #16517654.
Yersinia pestis
Yes
Flashner, Y. et al. (2004) Infect. Immun. 72, 908–15.
PubMed ID #14742535.
Burkholderia species
Yes
Moore, R.A. et al. (2004) Infect. Immun. 72, 4172–87.
PubMed ID #15213162.
Salmonella enterica
Yes
Nair, S. et al. (2004) J. Bacteriol. 186, 3214–23.
PubMed ID #15126484.
Field bacteria samples, non-Agrobacterium
Yes
Weller, S.A. et al. (2004) Appl. Environ. Microbiol. 70, 2779–85.
PubMed ID #15128532.
Haemophilus influenzae
Yes
Ottemann, K.M. and Lowenthal, A.C. (2002) Infect. Immun. 70, 1984–90.
PubMed ID #11895962.
Bordetella bronchiseptica
Yes
Brockmeier, S.L. et al. (2002) Infect. Immun. 70, 481–90.
PubMed ID #11796573.
Leptospira kirschneri
Yes
Matsunaga, J. et al. (2002) Infect. Immun. 70, 323–34.
PubMed ID #11748198.
Klebsiella pneumoniae
Yes
Yigit, H. et al. (2001) Antimicrob. Agents Chemother. 45, 1151–61.
PubMed ID #11257029.
Marine Geobacteraceae
Yes
Bacteria colonizing energy-harvesting anodes were scraped off, organically extracted and purified using the Wizard® Genomic DNA Purification Kit.
Bond, D.R. et al. (2002) Science 295, 483–5.
PubMed ID #11799240.
Serratia marcescens
Yes
Peccia, J. and Hernandez, M. (2001) Appl. Environ. Microbiol. 67, 4225–32.
PubMed ID #11526027.
Borrelia burgdorferi
Yes
Chaconas, G. et al. (2001) EMBO J. 20, 3229–37.
PubMed ID #11406599.
Campylobacter jejuni
Yes
Dorrell, N. et al.(2001) Genome Res. 11, 1706–15.
PubMed ID #11591647.
Desulfovibrio desulfuricans
Yes
Rapp-Giles, B.J. et al. (2000) Appl. Environ. Microbiol. 66, 671–7.
PubMed ID #10653734.
Vibrio cholerae
Yes
Stine, O.C. et al. (2000) Infect. Immun. 68, 7180–5.
PubMed ID #11083852.
Vibrio harveyi
Yes
Zhou, W. et al. (1999) Biochemistry 38, 16246–52.
PubMed ID #10587447.
Paracoccus denitrificans
Yes
Shearer, N. et al. (1999) J. Bacteriol. 181, 6907–13.
PubMed ID #10559155.
Campylobacter species
Yes
Before genomic DNA isolation, the bacteria were washed in TE buffer and resuspended in 1ml of TE buffer at ~1 × 109 cells/ml.
Duim, B. et al. (1999) Appl. Environ. Microbiol. 65, 2369–75.
PubMed ID #10347015.
Sphingomonas paucimobilis
Yes
Jiang, S.C. et al. (1998) Appl. Environ. Microbiol. 64, 535–42.
PubMed ID #9464390.
Flavobacterium species
Yes
Jiang, S.C. et al. (1998) Appl. Environ. Microbiol. 64, 535–42.
PubMed ID #9464390.
Bacteria – Gram Positive
Sample TypePromegaExternalComments
Staphylococcus epidermidis
Yes
Yes
Cells need to be treated with a lytic enzyme before lysis buffer addition. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section.
Reference:
Vandecasteele, S.J. et al. (2001) J. Bacteriol. 183, 7094–101.
PubMed ID #11717267.
Bacillus anthracis
Yes
Pomerantsev, A.P. et al. (2006) Infect. Immun. 74, 682–93.
PubMed ID #16369025.
Bacillus cereus
Yes
van Schaik, W. et al. (2004) J. Bacteriol. 186, 316–25.
PubMed ID #14702299.
Bacillus subtilis
Yes
Ben-Yehuda, S. and Losick, R. (2002) Cell 109, 257–66.
PubMed ID #12007411.
Staphylococcus, 12 strains
Yes
Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6.
PubMed ID #12904342.
Lactobacillus acidophilus
Yes
Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6.
PubMed ID #12904342.
Actinomyces naeslundii
Yes
Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6.
PubMed ID #12904342.
Streptococcus pneumoniae
Yes
Brown, J.S. et al. (2002) Infect. Immun. 70, 4389–98.
PubMed ID #12117949.
Bacteria – Other bacteria
Sample TypePromegaExternalComments
Treponema species
Yes
Cells were grown, centrifuged and washed three
times with PBS. Protocol for Gram negative bacteria ("Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria") was then followed.
Additional Resource:
Greene, S.R. and Stamm, L.V. (1998) Promega Notes 68, 30.
Methanococcus jannaschii
Yes
Löwe, J. and Amos, L.A. (1998) Nature 391, 203–6.
PubMed ID #9428770.
Methanosarcina mazei
Yes
Lange, M. et al. (2000) Appl. Environ. Microbiol. 66, 1796–1800.
PubMed ID #10788341.
Mycoplasma hyorhinis
Yes
Cells were collected and lysed in 1% sodium dodecyl sulfate (SDS), 45mM Tris (pH 8.0) and 9mM EDTA for 10 minutes at 50°C, then incubated for 10 minutes at 37°C. The Wizard® Genomic DNA Purification Kit was subsequently used for genomic DNA extraction.
Citti, C. et al. (2000) J. Bacteriol. 182, 1356–63.
PubMed ID #10671459.
Mycobacterium parafortuitum
Yes
Cells were treated with proteinase K and lysozyme for
1 hour, centrifuged and resuspended in Nuclei Lysis Solution. Glass beads were added, the glass bead-cell lysate shaken for 5 minutes, incubated at 65°C for 1 hour and the glass bead-lysate shaken again for 3 minutes. The protocol then followed the bacterial method of the Wizard® Genomic DNA Purification Kit ("Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria").
Peccia, J. and Hernandez, M. (2001) Appl. Environ. Microbiol. 67, 4225–32.
PubMed ID #11526027.
Intestinal microflora
Yes
Chicken intestines were removed and the contents of each segment (duodenum, jejunum, ileum, and cecum) were inverted into a sterile 15ml tube containing 9ml of sterile PBS. After adding 4mm glass beads, the samples were vortexed for 3 minutes, and the debris was removed by centrifuging at 700 × g for 1 minute. The supernatant was collected and centrifuged at 12,000 × g for 5 minutes. The pellet was washed twice with PBS and stored at –20°C until DNA extraction. This frozen pellet was resuspended in EDTA and treated with lysozyme (at a final concentration of 10mg/ml) for 45 minutes at 37°C. The bacterial genomic DNA was isolated with the Wizard® Genomic DNA Purification Kit.
Amit-Romach, E., Sklan, D. and Uni, Z. (2004) Poult. Sci. 83, 1093–98.
PubMed ID #15285498
Yeast
Sample TypePromegaExternalComments
Saccharomyces cerevisiae
Yes
Yes
Yeast need to be treated with lyticase prior to adding lysis buffer. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Yeast" section.
Reference:
Soteropoulos, P. and Perlin, D.S. (1998) J. Biol. Chem. 273, 26426–31.
PubMed ID #9756876.
Candida species, various
Yes
Yeast cells were incubated for 1 hour at 37°C with 1 M sorbitol, 0.1 M EDTA, 0.1% (w/v) zymolyase-100T (Seikaguku Corp.), and 1% (v/v) 2-mercaptoethanol adjusted to pH 7.5. Chromosomal DNA was then isolated using the Wizard® Genomic DNA Purification Kit.
Park, S. et al.(2000) J. Clin. Microbiol. 38, 2829–36.
PubMed ID #10921935.
Fungus
Sample TypePromegaExternalComments
Madurella mycetomatis
Yes
Mycelia were sonicated and ground in liquid nitrogen before the addition of Nuclei Lysis Solution.
Ahmed, A. et al. (2003) J. Clin. Microbiol. 41, 4537–41.
PubMed ID #14532179.
Aspergillus fumigatus
Yes
Cells were filtered through a 0.2µm SFCA filter, frozen on dry ice, then ground with a mortar and pestle. The ground cells were incubated in Nuclei Lysis Solution at 37°C for 1 hour before purifying with the Wizard® Genomic DNA Purification Kit," Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section.
Burghoorn, H.P. et al. (2002) Antimicrob. Agents Chemother. 46, 615–24.
PubMed ID #11850239.
Nectria haematococca
Yes
Mycelia were ground in liquid nitrogen and lyophilized before lysing in a modified lysis buffer consisting of 50 mM Tris-HCl (pH 7.5), 50 mM EDTA (pH 8.0), 3% SDS and 1% mercaptoethanol.
Wu, Q. et al. (1998) Mol. Biol. Cell 9, 89–101.
PubMed ID #9436993.
Pneumocystis carinii
Yes
Sputum samples were dispensed into ~1ml aliquots and centrifuged at 14,000 × g for 5–7 minutes. The cell pellet was resuspended in 1 ml of phosphate-buffered saline (0.01M, pH 7.2) containing 1mM EDTA (PBS-EDTA), washed twice in PBS-EDTA, centrifuged and stored at –80°C for later DNA extraction. DNA was prepared using the Wizard® Genomic DNA Purification Kit, "Isolating Genomic DNA from Whole Blood " section.
Beard, C.B. et al. (2000) Emerg. Infect. Dis. 6, 265–72.
PubMed ID #10827116.
Other Organisms and Tissues
Sample TypePromegaExternalComments
Single flour beetles (Tribolium castaneum)
Yes
Lorenzen, M.D. et al. (2002) Genetics 160, 225–34.
PubMed ID #11805058.
Drosophila, adult fly
Yes
To prepare genomic DNA from adult D. virilis and D. melanogaster, 100 adult flies were homogenized and the DNA purified using the Wizard® Genomic DNA Purification Kit.
Hanrahan, C.J. et al. (2000) Genetics 155, 1149–60.
PubMed ID #10880477.
Frog Skin
Yes
Skin biopsies from Amphibiocystidium ranae (10µm sections) were deparaffinized twice in xylene and centrifuged at high speed. The tissue pellet was washed with 95% and 70% ethanol, then dried, and the genomic DNA was extracted using the Wizard® Genomic DNA Purification Kit.
Pereira, C.N. et al. (2005) J. Clin. Microbiol. 43, 192–8.
PubMed ID #15634971.
Cichlid fin and whole fish
Yes
Genomic DNA was isolated from 1–2 mm2 ethanol-preserved Neolamprologus pulcher finclip samples or whole offspring using the Wizard® Genomic DNA Isolation Kit, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section, with small modifications (not listed).
Heg, D. et al. (2006) Behav. Ecol. 17, 419–29.
Plasmodium vivax
Yes
Researchers used a modified animal tissue/tail snip protocol.
Sawabe, T and Ikeda, T. (2005) Wizard® Genomic DNA Purification Kit provides high-quality genomic DNA template for molecular phylogenetic studies on Copepod crustaceans. eNotes
Plasmodium vivax
Yes
To isolate the parasite from whole blood, a modified protocol was used.
  1. Centrifuge 15ml vials containing infected sodium-citrate whole blood at 3,000rpm for 10 minutes.
  2. Extract serum and leukocytes with 5ml pipettes.
  3. Dilute erythrocytes in 7 volumes of 0.15% saponin.
  4. Incubate at 37°C for 1 hour.
  5. Centrifuge at 3,000rpm for 40 minutes.
  6. Discard supernatant, taking care not to disturb the parasite pellet.
  7. Resuspend the pellet in 15ml of 1X PBS and centrifuge at 3,000rpm for 10 minutes. Discard the supernatant, and repeat the procedure twice.
Start with the Nuclei Lysis Solution addition step in the "Isolating Genomic DNA from Whole Blood" section of the Wizard® Genomic DNA Purification Kit Technical Manual #TM050.
(Personal communication)
Amoeba
Yes
Trophozoites of clone A of Entamoeba histolytica (strain HM1:IMSS) were "axenically cultured" in TYI-S33 medium. Trophozoites (3 × 106) were processed as described in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, following the protocol for isolation of genomic DNA from tissue culture cells ("Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section). DNA pellet was dissolved with 50µl of DNA rehydration solution and incubated overnight at 4°C. Yield: 2.5µg per 3 × 106 trophozoites. Purity A260/280: 1.8.
(Personal communication)

Protocols

Specifications

You are viewing: A1120 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Cell Lysis Solution

A793A 1 × 100ml View Product

Nuclei Lysis Solution

A7941 1 × 50ml

Protein Precipitation Solution

A795A 1 × 25ml View Product

DNA Rehydration Solution

A796A 1 × 50ml View Product

RNase A Solution

A797A 1 × 250μl View Product

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Store at 22–25°C.

Specifications

You are viewing: A1125 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Cell Lysis Solution

A793A 5 × 100ml View Product

Nuclei Lysis Solution

A7941 5 × 50ml

Protein Precipitation Solution

A795A 5 × 25ml View Product

DNA Rehydration Solution

A796A 2 × 50ml View Product

RNase A Solution

A797A 5 × 250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Store at 22–25°C.

Specifications

You are viewing: A1620 Change Configuration

What's in the box?

Item Part # Size

Cell Lysis Solution (Genomic Purification)

A7933 3 × 1 liter

Nuclei Lysis Solution

A7943 1 × 1 liter

Protein Precipitation Solution

A7953 1 × 350ml

DNA Rehydration Solution

A7963 3 × 50ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Store at 22–25°C.

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