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ReliaPrep™ Large Volume HT gDNA Isolation System

A2715

Automated DNA Isolation from 1–10ml Blood

  • Effective recovery from pristine, hemolysed or frozen samples
  • Purified DNA ready for use in downstream assays or archiving
  • Chemistry scaled for each sample, reducing waste

Size

Catalog number selected: A2751

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ReliaPrep™ Large Volume HT gDNA Isolation System
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Scalable, Walkaway DNA Purification from Large-Volume Blood Samples

The ReliaPrep™ Large Volume HT gDNA Isolation System is used with the HSM 2.0 Instrument (Cat.# A2715) to provide a scalable, efficient method for isolating genomic DNA (gDNA) from 1–10ml blood samples. The precipitation-free ReliaPrep™ DNA purification chemistry eliminates the tedious centrifugation steps and hazardous chemicals associated with precipitation-based methods, reducing purification failures. Each kit provides enough reagents to process up to 96 × 10ml whole blood samples.  

The system has been automated on robotic liquid-handling workstations, allowing walkaway purification of 1–10ml whole blood, regardless of sample storage or shipping conditions.

Genomic DNA yields from 10ml normal whole blood samples are typically 200–400µg (depending on white blood cell count) in a final eluted volume of 1ml. Recovered DNA exhibits good purity with A260/A280 ratios greater than 1.7 and A260/A230 ratios between 1.8 and 2.2.

Products may not be available in all countries.

11276CA-Transparent

Expedite Sample Processing with the HSM 2.0 Heater Shaker Magnet

The HSM 2.0 Instrument heats, shakes and magnetizes sample tubes in one position throughout the extraction process from lysis to elution, eliminating the need to move samples on the robot deck and reducing instrument failures.

For low-throughput isolation of gDNA from up to 32 samples at one time, the HSM 2.0 instrument can be used in a semi-automated mode, where the user performs the pipetting functions. The HSM has software that controls the instrument and directs the user through the purification protocol.

No Manual Sample Transfer Once Instrument Is Loaded

Simply place the primary blood sample tubes on the instrument or transfer samples to processing tubes, follow the setup instructions and start the protocol. Purified DNA is ready in ≤4.5 hours. 

Automated DNA purification from Blood, reliaprep method 8525MB-W

Process Multiple Sample Types

See the Application Notes linked below for details on purification of DNA from blood, buffy coat and packed cell pellet  samples using the ReliaPrep™ Large Volume HT gDNA Isolation System.
Sample TypePromegaExternalComments/Reference
Human Whole Blood
Yes
No
Isolating gDNA from Blood: Simplified gDNA Isolation from Human Blood using the ReliaPrep™ Large Volume HT gDNA Isolation System. Application Note AN186, Promega Corporation
Buffy Coat
Yes
No
Isolating gDNA from Buffy Coat Samples: Semi-Automated or Fully Automated Isolation of gDNA from Buffy Coat Samples Using the ReliaPrep™ Large Volume HT gDNA Isolation System. Application Note AN185, Promega Corporation
Packed Cell Pellets
Yes
No
Isolating gDNA from Packed Cell Pellets: Simplified gDNA Isolation from Human Blood Packed Cell Pellets Using the ReliaPrep™ Large Volume HT gDNA Isolation System. Application Note AN203, Promega Corporation.
Reagents Available Separately
ProductSizeCat.#
RNase A Solution
4mg/ml
20X TE Buffer (pH 7.5)
25ml
Tissue Lysis Buffer (TLA)
500ml
Nuclease-Free Water
1,000ml
Integrated Reagent Caps
4/pk
Bottle for 50% Ethanol
HSM 2.0 Ordering Information
ProductCat.#
HSM 2.0 Instrument
Service Agreements
ProductCat.#
ReliaPrep™ LV 32 HSM Standard Service Agreement

Protocols

Specifications

What's in the box?

Item Part # Size

Alkaline Protease (APA)

A1721 1 × 130ml

Cell Lysis Buffer (CLD)

A1731 1 × 1,400ml

Binding Buffer (BBA)

A1741 1 × 1,600ml

ReliaPrep™ Resin

A1752 1 × 115ml

Prepared Wash Buffer (WBC)

A2681 1 × 3,500ml

Proteinase K (PK) Solution

A5051 1 × 23ml

Nuclease-Free Water

P1195 4 × 150ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. No. 6,855,499 and other patents.

Resources

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Biobanking Workflow Solutions

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