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Citations Search

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Cancer Genetics . Challenges in Next Generation Sequencing Analysis of Somatic Mutations in Transplant Patients 2018

Chen, H., Luthra, R., Patel, K.P., Routbort, M., Rashid, A., Roy-Chowdhuri, S., Lazar, A., Broaddus, R., Manekia, J., Singh, R.R. and Yemelyanova, A.

Notes: ReliaPrep Large Volume HT gDNA Isolation System was used for DNA extraction from peripheral blood samples (a default source of germline DNA) from tumor cells. The DNA was amplified, sequencing library preparation and next-generation sequencing was also performed in order to analyze somatic mutations in solid tumors and hematologic malignancies. (4979)

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PLos ONE 12. Effect of Pre-Analytic Variables on the Reproducibility of qPCR Relative Telomere Length Measurement 2017

Dagnall, C., Hicks, B., Teshome, K., Hutchinson, A.A., Gadalla S.M., Khincha, P.P., Yeager, M. and Savage, S.A.

Notes: ReliaPrep Large Volume HT gDNA Isolation System was used to extract DNA from 1 of 3 homogenous aliquots made from buffy coat specimens at the Frederick National Laboratory for Cancer Research. ReliaPrep was used for its magnetic bead/particle-based methods, while the QIAamp kit (also used) used a silica-membrane-based nucleic acid purification method. The DNA extracted was then quantified and PCR was performed using non-Promega products. (4973)

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J. Mol. Diagn. 19, 255–264. A Targeted High-Throughput Next-Generation Sequencing Panel for Clinical Screening of Mutations, Gene Amplifications, and Fusions in Solid Tumors 2016

Luthra, R., Patel, K.P., Routbort, M.J., Broaddus, R.R., Yau, J., Simien, C., Chen, W., Hatfield, D.Z., Medeiros, L.J. and Singh, R.R.

Notes: In a study of 121 tumors, ReliaPrep Large Volume HT gDNA Isolation System was used for nucleic acid extraction from 24 peripheral blood samples. Sequencing library preparation, quantification and next-generation sequencing was performed using the extracted samples and non-Promega products. (4974)

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