Usage Note: Ends generated with XhoI can be directly ligated to ends generated by SalI. Neither recognition site is regenerated in the ligation product.
Incubation Conditions: Buffer D. 37°C.
Source: Xanthomonas holcicola.
Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM KCl, 0.1% Triton® X-100, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.