- Sensitive to overlapping dam methylation.
- Ends generated with XbaI can be directly ligated to ends generated by SpeI or NheI. None of these recognition sites are regenerated in the ligation product.
Incubation Conditions: Buffer D. 37°C.
Source: Xanthomonas badrii.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.