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SpeI

SpeI GQ 200u
customize-this-small
View information on global supply logistics

  • Capable of digesting DNA in 15 minutes or less
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Genome qualified to ensure optimal performance in genomic analysis applications

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Catalog number selected: R6591

$ 110.00
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This product is discontinued
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This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
SpeI
10u/µl/200u
$ 110.00
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Change Configuration

Cut Site:

A▼CTAG   T
T   GATC▲A

Usage Note: Ends generated with SpeI can be directly ligated to ends generated by NheI or XbaI. None of these recognition sites are regenerated in the ligation product.

Incubation Conditions: Buffer B. 37°C.

Source: Sphaerotilus natans ATCC 13923.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D MULTI-CORE™
75–100% 100% 75–100% 75–100% 100%

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
0 3 0 0 0 0

Protocols

Complete Protocol

Specifications

You are viewing: R6591 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Buffer B 10X Buffer

 R002A 1 × 1ml

Bovine Serum Albumin, Acetylated

 R396D 1 × 150μl 10mg/ml View Product

SpeI

 R659A 1 × 200u

MULTI-CORE™ 10X Buffer

 R999A 1 × 0.25ml View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: R6595 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Buffer B 10X Buffer

 R002A 1 × 1ml

Bovine Serum Albumin, Acetylated

 R396D 1 × 150μl 10mg/ml View Product

SpeI

 R659D 1 × 1,000u

MULTI-CORE™ 10X Buffer

 R999A 1 × 0.25ml View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Other Resources

Similar Products

XbaI

Restriction enzyme that cuts DNA at 37°C and leaves a 5´ overhang.

R6181, R6185

Frequently Used With

LigaFast™ Rapid DNA Ligation System

Efficiently ligates DNA inserts into plasmid vectors in just 5–15 minutes at room temperature.

M8221, M8225

Blue/Orange Loading Dye, 6X

Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

G1881

Ethidium Bromide Solution, Molecular Grade

Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients.

H5041

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