Usage Note: Ends generated with SpeI can be directly ligated to ends generated by NheI or XbaI. None of these recognition sites are regenerated in the ligation product.
Incubation Conditions: Buffer B. 37°C.
Source: Sphaerotilus natans ATCC 13923.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.