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- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
- Ends generated by PstI can be directly ligated to ends generated by NsiI. Neither restriction site is regenerated in the ligation product.
Incubation Conditions: Buffer H. 37°C.
Source: Recombinant E. coli strain.
Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.