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PstI

  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning qualified, provides a higher level of quality control for enzymes used in cloning applications

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Size

Catalog number selected: R6111

$ 38.00
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This product is discontinued
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PstI
10u/µl/3,000u
$ 38.00
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Change Configuration

Cut Site:

C   TGCAG
GACGT   C

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Notes:

  1. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
  2. Ends generated by PstI can be directly ligated to ends generated by NsiI. Neither restriction site is regenerated in the ligation product.

Incubation Conditions: Buffer H. 37°C.

Source: Recombinant E. coli strain.

Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D H MULTI-CORE™
10–25% 50–75% 50–75% 50–75% 100% 25–50%

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
28 30 1 1 1 1

Specifications

You are viewing: R6111 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer H 10X Buffer

R008A 1 × 1ml

Bovine Serum Albumin, Acetylated

R396D 1 × 150μl10mg/ml View Product

PstI

R611A 1 × 3,000u

MULTI-CORE™ 10X Buffer

R999A 1 × 0.25ml View Product

SDS

Choose language:

Download SDSPDF (116 KB) – English (United States)

Certificate of Analysis

See all certificates for R6111

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: R6115 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer H 10X Buffer

R008A 1 × 1ml

Bovine Serum Albumin, Acetylated

R396E 1 × 750μl10mg/ml View Product

PstI

R611B 1 × 15,000u

MULTI-CORE™ 10X Buffer

R999A 1 × 0.25ml View Product

SDS

Choose language:

Download SDSPDF (116 KB) – English (United States)

Certificate of Analysis

See all certificates for R6115

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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Chris

Chris

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