- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
- Ends generated with NheI can be directly ligated to ends generated by SpeI or XbaI. None of these recognition sites are regenerated in the ligation product.
Incubation Conditions: Buffer B. 37°C.
Source: Neisseria mucosa heidelbergensis.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.