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NdeI

R6801_NdeI--500u_3
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  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix

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Catalog number selected: R6801

$ 163.00
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NdeI
10u/µl/500u
$ 163.00
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Cut Site:

CATA  TG
GT   ATAC

Notes:

  1. Star activity may be observed with glycerol concentrations >10%.
  2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
  3. Ends generated with NdeI can be directly ligated to ends generated by VspI. Neither recognition site is regenerated in the ligation product.

Incubation Conditions: Buffer D. 37°C.

Source: Neisseria denitrificans.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D MULTI-CORE™
<10% <10% 25–50% 100% 25–50%

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
7 2 0 1 3 1

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Buffer D 10X Buffer

R004A 1 × 1ml

Bovine Serum Albumin, Acetylated

R396D 1 × 150μl 10mg/ml View Product

NdeI

R680A 1 × 500u

MULTI-CORE™ 10X Buffer

R999A 1 × 0.25ml View Product

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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