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MluI

Part Numbers: R6381

R6381_MluI--GQ--1-000u_3
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  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Genome qualified to ensure optimal performance in genomic analysis applications

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MluI
10u/µl/1,000u
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Cut Site:

A▼CGCG   T
T   GCGC▲A

Usage Note: Ends generated using MluI can be directly ligated to ends generated by BssHII. Neither restriction site is regenerated in the ligation product.

Incubation Conditions: Buffer D. 37°C.

Source: Micrococcus luteus.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D MULTI-CORE™
10–25% 25–50% 50–75% 100% 10–25%

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
7 5 2 0 0 0

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Buffer D 10X Buffer

 R004A 1 × 1ml

Bovine Serum Albumin, Acetylated

 R396D 1 × 150μl 10mg/ml View Product

MluI

 R638A 1 × 1,000u

SDS

Search for SDS

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Citations

Other Resources

Frequently Used With

LigaFast™ Rapid DNA Ligation System

Efficiently ligates DNA inserts into plasmid vectors in just 5–15 minutes at room temperature.

M8221, M8225

Blue/Orange Loading Dye, 6X

Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

G1881

Agarose, LE, Analytical Grade

Reagent for electrophoretic separation of nucleic acids.

V3121, V3125

Ethidium Bromide Solution, Molecular Grade

Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients.

H5041

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