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Note: Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratios >25u/μg, in the absence of NaCl, in the presence of Mn2+, or under high pH conditions.
Incubation Conditions: Buffer H. 37°C.
Source: Escherichia coli RY 13.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 400mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.