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BamHI

  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Available at high concentration, containing 12,500 units of BamHI at a concentration of 40–80u/μl

Choose a concentration

Size

Catalog number selected: R6021

$ 50.00
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BamHI
10u/µl/2,500u
$ 50.00
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Change Configuration

Cut Site:

GGATC  C
C   CTAGG

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Usage Notes:

  1. Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn2+.
  2. Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product.

Incubation Conditions: Buffer E. 37°C.

Source: Bacillus amyloliquefaciens H.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 5mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D E MULTI-CORE™
75–100%* 75–100% 75–100% 50–75% 100% 75–100%

*Buffer A is not recommended due to potential star activity.

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
5 3 0 1 1 1

Specifications

You are viewing: R6021 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer E 10X Buffer

R005A 1 × 1ml

Bovine Serum Albumin, Acetylated

R396D 1 × 150μl10mg/ml View Product

BamHI

R602A 1 × 2,500u

MULTI-CORE™ 10X Buffer

R999A 1 × 0.25ml View Product

SDS

Choose language:

Download SDSPDF (91 KB) – English (United States)

Certificate of Analysis

See all certificates for R6021

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: R6025 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer E 10X Buffer

R005A 1 × 1ml

Bovine Serum Albumin, Acetylated

R396E 1 × 750μl10mg/ml View Product

BamHI

R602B 1 × 12,500u

MULTI-CORE™ 10X Buffer

R999A 1 × 0.25ml View Product

SDS

Choose language:

Download SDSPDF (115 KB) – English (United States)

Certificate of Analysis

See all certificates for R6025

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: R4024 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer E 10X Buffer

R005A 1 × 1ml

Bovine Serum Albumin, Acetylated

R396E 1 × 750μl10mg/ml View Product

BamHI (HC)

R402A 1 × 12,500u

MULTI-CORE™ 10X Buffer

R999A 1 × 0.25ml View Product

SDS

Choose language:

Download SDSPDF (116 KB) – English (United States)

Certificate of Analysis

See all certificates for R4024

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: R4027 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer E 10X Buffer

R005A 1 × 1ml

Bovine Serum Albumin, Acetylated

R396F 1 × 1,250μl10mg/ml View Product

BamHI (HC)

R402B 1 × 50,000u

MULTI-CORE™ 10X Buffer

R999B 1 × 1ml View Product

SDS

Choose language:

Download SDSPDF (115 KB) – English (United States)

Certificate of Analysis

See all certificates for R4027

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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