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AgeI

R7251_AgeI-100u_3
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  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix

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Catalog number selected: R7251

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AgeI
3–10u/µl/100u
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Cut Site:

ACCGG   T
T   GGCCA

Usage Note: Ends generated with AgeI can be directly ligated to ends generated using AccIII, NgoMIV or XmaI. The AgeI recognition site is not regenerated in the ligation product.

Incubation Conditions: Buffer K. 37°C.

Source: Agrobacterium gelatinovorum.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol, 0.5mg/ml BSA.

Percent Activity in 4-CORE® Buffer:

A B C D K MULTI-CORE™
25–50% 25–50% 25–50% 50–75% 100% 100%

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
13 5 0 0 0 0

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Buffer K 10X Buffer

R010A 1 × 1ml

Bovine Serum Albumin, Acetylated

R396D 1 × 150μl 10mg/ml View Product

AgeI

R725A 1 × 100u

MULTI-CORE™ 10X Buffer

R999A 1 × 0.25ml View Product

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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