We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

T4 RNA Ligase

Joins ssRNA or ssDNA onto the 5´-Phosphoryl End of ssRNA or ssDNA

  • Adds [5´-32P] nucleoside 3´,5´-bis (phosphate) onto single-stranded RNA
  • Can be inactivated by heating at 65°C for 15 minutes
  • Supplied at a concentration of 10u/μl

Size

Catalog number selected: M1051

$ 132.00 Your price: Log in

Add to Helix
T4 RNA Ligase
500u
$ 132.00
Your price: Log in

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5´-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. T4 RNA Ligase is provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 50mM DTT, 10mM ATP.

Applications

  • 3´ end-labeling of RNA.
  • Circularization of RNA and DNA oligonucleotides.
  • Cloning full-length cDNA.

References

  1. Silber, R. et al. (1972) Proc. Natl. Acad. Sci. USA 69, 3009–13.
  2. England, T. et al. (1977) Proc. Natl. Acad. Sci. USA 74, 4839–42.
  3. Uhlenbeck, O.C. and Gumport, R.I. (1982) The Enzymes 158, 31.
  4. Brennan, C.A. et al. (1983) Meth. Enzymol. 100, 38–52.
  5. Schaefer, B.C. (1995) Anal. Biochem. 227, 255–73.

Storage Buffer: 10mM Tris (pH 7.5 at 25°C), 50mM KCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.1% Tween® 20.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease.

Unit Definition: One unit catalyzes the formation of 1nmol of [5´-32P]rA14–20 into a phosphatase-resistant form in 30 minutes at 37°C in a total volume of 30μl. The reaction conditions are: 50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 5mM DTT, 1mM ATP and 10μM [5´-32P]rA14–20.

Protocols

Specifications

What's in the box?

Item Part # Size

T4 RNA Ligase

M105A 1 × 500u

T4 RNA Ligase 10X Buffer

M107A 1 × 500μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB
Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom