The pUC/M13 Primers are used to sequence inserts cloned into the M13 vectors and pUC plasmids developed by Messing. The primers are purified by gel electrophoresis or HPLC and supplied in sterile water.
- Forward (17mer): 5´-d(GTTTTCCCAGTCACGAC)-3´
- Reverse (17mer): 5´-d(CAGGAAACAGCTATGAC)-3´
- Reverse (22mer): 5´-d(TCACACAGGAAACAGCTATGAC)-3´
- Forward (24mer): 5´-d(CGCCAGGGTTTTCCCAGTCACGAC)-3´
- Messing, J. (1983) Meth. Enzymol. 101, 20–78.