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pGEM®-3Zf(+/–) Vectors

P2261_pGEM--3Zf----Vector--20ug_3
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Standard Cloning Vectors That Can Produce ssDNA and In vitro Transcribe Sense and Antisense RNA

  • Blue/white screening for easy identification of recombinant clones
  • In vitro transcription using the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
  • The difference between + and – vectors is orientation of the f1 origin

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Catalog number selected: P2271

$ 118.00
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pGEM®-3Zf(+/–) Vectors
pGEM®-3Zf(+) Vector/20µg
$ 118.00
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The pGEM®-3Zf(+) and pGEM®-3Zf(–) Vectors contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII. The pGEM®-3Zf(+) and -3Zf(–) Vectors can be used as standard cloning vectors and as templates for in vitro transcription.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0282VC05_4A-W

pGEM®-3Zf(+) Vector GenBank® Accession Number X65306 and vector sequence text file.

0282VB05_4A-W

pGEM®-3Zf(–) Vector GenBank® Accession Number X65307 and vector sequence text file.

Specifications

You are viewing: P2271 Change Configuration

What's in the box?

Item Part # Size

pGEM®-3Zf(+) Vector

P227A 1 × 20μg

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Specifications

You are viewing: P2261 Change Configuration

What's in the box?

Item Part # Size

pGEM®-3Zf(–) Vector

P226A 1 × 20μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

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