Conventional DNA Digest Markers are created by digesting DNA (i.e., λ DNA, ΦX174 replicative form DNA or plasmids) to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. These markers are not intended for quantitative analysis.
The pGEM® DNA Markers have 15 phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These unique markers are generated from separate digests of pGEM®-3 Vector DNA with HinfI, RsaI and AvaII later combined to form the markers.
Storage Buffer: 10mM Tris-HCl (pH 7.8), 1mM EDTA.
Now shipping at ambient temperature. Learn more about the quality testing in the white paper.