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pGEM®-7Zf(+/–) Vectors

Standard Cloning Vectors and Templates for In vitro Transcription and Circular ssDNA Production

  • Blue/white screening for easily identifying recombinant clones
  • In vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
  • Orientation of the f1 origin differentiates vectors (+ and –)

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Catalog number selected: P2251

$ 134.00
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This product is discontinued
pGEM®-7Zf(+/–) Vectors
pGEM®-7Zf(+) Vector/20µg
$ 134.00
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The pGEM®-7Zf(+) and pGEM®-7Zf(–) Vectors contain SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-peptide coding region of β-galactosidase. This arrangement of promoters means both sense and antisense RNA can be produced. Insertional inactivation of the α-peptide identified recombinant clones by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. pGEM®-7Zf(+) and pGEM®-7Zf(–) Vectors are identical except for the orientation of the f1 origin.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0286VC-W

0286VB02_5A-W

pGEM®-7Zf(–) Vector GenBank® Accession Number X65311 and vector sequence text file.

Specifications

You are viewing: P2251 Change Configuration

What's in the box?

Item Part # Size

pGEM®-7Zf(+) Vector

P225A 1 × 20μg

SDS

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Download SDSPDF (67 KB) – English (United States)

Certificate of Analysis

See all certificates for P2251

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Specifications

You are viewing: P2371 Change Configuration

What's in the box?

Item Part # Size

pGEM®-7Zf(–) Vector

P237A 1 × 20μg

SDS

Choose language:

Download SDSPDF (67 KB) – English (United States)

Certificate of Analysis

See all certificates for P2371

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

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Manas

Manas

India