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pGEM®-7Zf(+/–) Vectors

Standard Cloning Vectors and Templates for In vitro Transcription and Circular ssDNA Production

  • Blue/white screening for easily identifying recombinant clones
  • In vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
  • Orientation of the f1 origin differentiates vectors (+ and –)

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Size

Catalog number selected: P2371

$ 131.00 Your price: Log In

pGEM®-7Zf(+/–) Vectors
pGEM®-7Zf(–) Vector/20µg
$ 131.00
Your price: Log In
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The pGEM®-7Zf(+) and pGEM®-7Zf(–) Vectors are derivatives of the pGEM®-3Zf(+) Vector and contain the origin of replication of the filamentous phage f1. These plasmids contain SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-peptide coding region of β-galactosidase. This arrangement of promoters means both sense and antisense RNA can be produced. Insertional inactivation of the α-peptide identified recombinant clones by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. pGEM®-7Zf(+) and pGEM®-7Zf(–) Vectors are identical except for the orientation of the f1 origin.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0286VC-W

0286VB02_5A-W

pGEM®-7Zf(–) Vector GenBank® Accession Number X65311 and vector sequence text file.

Protocols

Specifications

You are viewing: 20μg Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

pGEM®-7Zf(–) Vector

P237A 1 x 20μg

Bacterial Strain JM109

P975A 1 x 500μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

Specifications

You are viewing: 20μg Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

pGEM®-7Zf(+) Vector

P225A 1 x 20μg

Bacterial Strain JM109

P975A 1 x 500μl View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

Resources

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