Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

LigaFast™ Rapid DNA Ligation System

M8221_LigaFast--Rapid-DNA-Ligation-System_3

Ligate DNA Inserts into Plasmid Vectors in 15 Minutes or Less at Room Temperature

  • DNA inserts with blunt ends or 5´ or 3´ overhangs can be used for ligation
  • No purification of ligated DNA needed prior to heat-shock transformation in E. coli
  • Ligations performed using the LigaFast™ System are comparable to standard overnight ligations

Size

Catalog number selected: M8221

$ 91.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
LigaFast™ Rapid DNA Ligation System
30 reactions
$ 91.00
Your price: Log in
Change Configuration

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.

The LigaFast™ System is Blue/White Cloning Qualified, providing a higher level of quality control for enzymes used in cloning applications.

Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

Specifications

You are viewing: M8221 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

2X Rapid Ligation Buffer

C671A 1 × 200μl View Product

T4 DNA Ligase

M180A 1 × 100u

SDS

Choose language:

Download SDSPDF (218 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: M8225 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

2X Rapid Ligation Buffer

C671B 2 × 500μl View Product

T4 DNA Ligase

M180B 1 × 500u

SDS

Choose language:

Download SDSPDF (220 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-stephen

Stephen

UK