Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Flexi® Enzyme Blend (SgfI & PmeI)

R1851_10X-Flexi--Enzyme-Blend--Sgf-I-and-Pme1-_3
Click here to customize this product

Reagent Used with Flexi® Cloning System

  • Required to screen Flexi® Vector clones for presence of protein-coding region of interest
  • Also can be used to cleave PCR products prior to cloning into Flexi® Vectors or transfer protein-coding regions between Flexi® Vectors

Size

Catalog number selected: R1851

$ 128.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Flexi® Enzyme Blend (SgfI & PmeI)
25μl
$ 128.00
Your price: Log in
Change Configuration

The 10X Flexi® Enzyme Blend (SgfI and PmeI) is required to screen Flexi® Vector clones for the presence of the protein-coding region of interest. This enzyme blend can also be used to cleave PCR products prior to cloning into the Flexi® Vectors or transfer protein-coding regions between Flexi® Vectors. For more information see the Flexi® Vector Systems Technical Manual #TM254.

Recognition Sites:
SgfI recognition site:
5´…GCG   AT▼CGC…3´
3´…CGC▲TA   GCG…5´

PmeI recognition site:
5´…GTTT▼AAAC…3´
3´…CAAA▲TTTG…5´

Storage Buffer: The 10X Flexi® Enzyme Blend (SgfI and PmeI) is supplied in 10mM Tris-HCl (pH 7.4 at 25°C), 150mM KCl, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.35mg/ml BSA, 0.05% Thesit® and 50% glycerol.

5X Flexi® Digest Buffer: The composition of 5X Flexi® Digest Buffer is 50mM Tris-HCl (pH 7.9 at 37°C), 50mM MgCl2, 250mM NaCl, 5mM DTT, 0.5mg/ml acetylated BSA.

Specifications

You are viewing: R1851 Change Configuration

What's in the box?

Item Part # Size Available Separately

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Flexi® Enzyme Blend (SgfI & PmeI)

R185A 1 × 25μl

5X Flexi® Digest Buffer

R186A 1 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Specifications

You are viewing: R1852 Change Configuration

What's in the box?

Item Part # Size Available Separately

Nuclease-Free Water

P119A 2 × 1,250μl View Product

10X Flexi® Enzyme Blend (SgfI & PmeI)

R185B 1 × 100μl

5X Flexi® Digest Buffer

R186A 1 × 1ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Let's find the product that meets your needs.

Talk to a Scientist

Robert

Robert

US