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JM109 Competent Cells

L1001

Ready-to-Use Competent E. coli Cells for Recombinant Cloning and Subcloning

  • Cells are ready to use—no preparation time necessary
  • Transformation efficiencies guaranteed
  • No worries about undesirable recombination events (recA– mutation), and no nuclease carryover in miniprep DNA (endA– mutation)

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Catalog number selected: L2005

$ 182.00 Your price: Log In

JM109 Competent Cells
Single use/1ml
(20 × 50µl)
$ 182.00
Your price: Log In
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JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg for Cloning and Single Use, and Subcloning Efficiency at greater than 107cfu/μg.

For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.

JM109 Competent Cells are prepared according to a modified procedure of Hanahan.

JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].

Reference

  1. Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.

Protocols

Specifications

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Item Part # Size

Single-Use JM109 Competent Cells, >108cfu/µg

L200B 20 × 50μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L1001 Change Configuration

What's in the box?

Item Part # Size

JM109 Competent Cells, Subcloning Efficiency

L100A 5 × 200μl

Competent Cells Control DNA

L107A 1 × 30μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L2001 Change Configuration

What's in the box?

Item Part # Size

Competent Cells Control DNA

L107A 1 × 30μl

JM109 Competent Cells, High Efficiency

L200A 5 × 200μl

SDS

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Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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