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HB101 Competent Cells

Ready-to-Use Competent E. coli Cells for Recombinant Cloning and Subcloning

  • Cells are ready to use—no preparation time necessary
  • Transformation efficiencies guaranteed
  • No worries about undesirable recombination events (recA– mutation)

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Size

Catalog number selected: L2015

$ 160.00 Your price: Log In

HB101 Competent Cells
Single use/1ml
(20 × 50µl)
$ 160.00
Your price: Log In
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HB101 cells are recA–, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable for vectors that do not require α-complementation for blue/white screening.

For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.

HB101 Competent Cells are prepared according to a modified procedure of Hanahan.

HB101 Genotype: F–, thi-1, hsdS20 (rB, mB), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1.

Reference

  1. Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.

Protocols

Specifications

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What's in the box?

Item Part # Size

Single-Use HB101 Competent Cells, >108cfu/µg

L201B 20 × 50μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L2011 Change Configuration

What's in the box?

Item Part # Size

Competent Cells Control DNA

L107A 1 × 30μl

HB101 Competent Cells, High Efficiency

L201A 5 × 200μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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