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Non-Radioactive Phosphatase Assay Systems

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Absorbance-Based Detection of Phosphatase Activity

  • Measure serine/threonine phosphatase activity
  • Use with purified samples, crude cell or tissue extracts
  • Convenient alternative to radioactive phosphatase assays


Catalog number selected: V2460

$ 578.00
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Non-Radioactive Phosphatase Assay Systems
96 reactions
$ 578.00
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Fast, Convenient Assays for Ser/Thr Phosphatase Activity

The Non-Radioactive Phosphatase Assay Systems determine the amount of free phosphate generated in a reaction by measuring the absorbance of a molybdate:malachite green:phosphate complex. They allow use of a variety of buffer conditions and substrates, including naturally phosphorylated proteins or synthetic phosphopeptides.

The effective range for the detection of phosphate released during an assay using the Phosphatase Assay Systems is 100–4,000pmol of phosphate.

Use with Purified Samples or Crude Cell and Tissue Extracts

In addition to measuring phosphatase activity in partially fractionated and purified samples, the Phosphatase Assay Systems can measure phosphatase activity in crude cell or tissue extracts. For this application, the high concentration of phosphate in these preparations is eliminated prior to performing the assay using the supplied Spin Columns, which rapidly and effectively remove free phosphate and other low-molecular-weight inhibitors from the sample. In addition, a unique Molybdate Dye Additive that is combined with the Molybdate Dye Solution aids in the solubilization of proteins exposed to the acid conditions of the Molybdate Dye Solution, which alone could potentially cause precipitation of the proteins.

Serine/Threonine Phosphatase Assay System

Contains the chemically synthesized phosphopetide, RRA(pT)VA, a peptide substrate that is compatible with several serine/threonine phosphatases such as the Protein Phosphatases 2A, 2B, and 2C. However the supplied phosphopeptide is a poor substrate for Protein Phosphatase 1 because of its more stringent structural requirements.


What's in the box?

Item Part # Size

Ser/Thr Phosphopeptide RRA(pT)VA

V248A 1 × 1mg

Spin Column, Adaptor, and Reservoir

V249A 4 × 1 each

Phosphate Standard

V250A 1 × 1ml

96-Well Plate (1/2 area, flat bottom)

V252A 1 × 1 each

Molybdate Dye Solution

V253A 1 × 20ml

Molybdate Dye Additive

V254A 1 × 200μl

Sephadex® G-25

V255A 1 × 40ml

Phosphate-Free Water

V654A 1 × 25ml

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


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