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Tracer Dilution Buffer

Part Numbers: N2191

N2191_Tracer-Dilution-Buffer_3
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For use with NanoBRET® Target Engagement Assays

  • Required to maintain solubility of NanoBRET® TE Tracers
  • Component of NanoBRET® Target Engagement Assays available for purchase separately

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Tracer Dilution Buffer
50ml
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Tracer Dilution Buffer is required to maintain solubility of concentrated solutions of NanoBRET® Intracellular TE Tracers when diluting the tracers into an aqueous solution. NanoBRET® Intracellular TE Tracers are diluted to a 20X stock concentration in Tracer Dilution Buffer prior to addition to the cell culture medium.

Specifications

What's in the box?

Item Part # Size

Tracer Dilution Buffer

 N219B 1 × 50ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Frequently Used With

NanoBRET® Target Engagement BET BRD Assays

Directly measure BET bromodomain-test compound affinity and residence time in live cells.

N2131, N2180, N2130, N2140

NanoBRET® TE Intracellular E3 Ligase Assays

Measure compound binding or affinity for an E3 ubiquitin ligase protein, CRBN or VHL, in live cells.

N2910, N2911, N2912, N2930, N2931, N2932, CS1810E20, CS1810E21, CS1810E23, CS1810E24

NanoBRET® Target Engagement Kinase Assays

Directly measure kinase-ligand binding affinity, occupancy and residence time in live cells.

N2520, N2521, N2540, N2501, N2500, N2530, N2600, N2601, N2620, N2621, N2630, N2631, N2640, N2641, N2650, N2651, NF1001, N2810, N2820, N2830, N2840, N2850, NF1200

NanoBRET® Target Engagement HDAC Assays

Directly measure HDAC-test compound affinity and residence time in live cells.

N2080, N2081, N2170, N2090

Intracellular TE Nano-Glo® Substrate/Inhibitor

The recommended NanoLuc® substrate and extracellular inhibitor for NanoBRET® Target Engagement Intracellular Assays.

N2162, N2160, N2161

Transfection Carrier DNA

A plasmid DNA used to reduce the amount of an expression vector or reporter vector in mammalian cell transfection while maintaining the overall amount of DNA.

E4881, E4882

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