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AKT3 Kinase Enzyme System

Kinase Enzyme Systems for Detecting Kinase Activity

Easily Screen and Profile AKT3 Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

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Catalog number selected: V4010

$ 465.00
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This product is discontinued
AKT3 Kinase Enzyme System
10µg
$ 465.00
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The AKT3 Kinase Enzyme System contains:

  • AKT3 Kinase, 10μg (Human, recombinant full-length). MW: ~85kDa.
  • AKT (SGK) Peptide Substrate (RPRAATF); derived from the N-terminus of GSK3.
  • Reaction Buffer, DTT.

Recombinant full-length human AKT3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT3 or Protein Kinase B γ (PKBγ) is a serine/threonine kinase that is a member of the AKT family. AKT3 is activated in cells exposed to stimuli such as hormones, growth factors and extracellular matrix components. AKT3 phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis and proliferation.

AKT3 NCBI Database Entry

The AKT3 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

Specifications

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SDS

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Download SDSPDF (231 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

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SDS

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Download SDSPDF (257 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: V4011 Change Configuration

What's in the box?

Item Part # Size
AKT3 Kinase Enzyme System V4010 1 × 10μg

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

SDS

Choose language:

Download SDSPDF (411 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

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